Development and validation of spectrophotometric method of cefpodoxime proxetil using hydrotropic solubilizing agents

PURPOSE: To develop and validate specific and accurate UV spectrophotometric method of cefpodoxime proxetil by using different hydrotropic solubilizing agents. MATERIALS AND METHODS: The present study deals with spectrophotometric analysis of cefpodoxime proxetil by utilizing 4 different hydrotropic agents such as ammonium acetate (6 M), sodium citrate (1.25 M), sodium gycinate (1 M), sodium chloride (1 M), and urea (1 M). RESULTS: From different hydrotropic agents, urea showed best aqueous solubility of cefpodoxime proxetil. The linearity was observed in the concentration range of 10-120 μg/ml. The method was validated and found to be precise. Accuracy (percent recovery) for cefpodoxime proxetil was found to be 99.82 ± 0.106. CONCLUSION: Urea as hydrotropic agent showed best aqueous solubility of cefpodoxime proxetil, which can be used as solubilizing agent. The proposed method is new, simple, safe, eco-friendly, economic, accurate, and cost-effective and can be successfully employed in routine analysis.