Paclitaxel-loaded poly(glycolide-co-ε-caprolactone)-b-D-α-tocopheryl polyethylene glycol 2000 succinate nanoparticles for lung cancer therapy

In order to improve the therapeutic efficacy and minimize the side effects of lung cancer chemotherapy, the formulation of paclitaxel-loaded poly(glycolide-co-ε-caprolactone)-b-D-α-tocopheryl polyethylene glycol 2000 succinate nanoparticles (PTX-loaded [PGA-co-PCL]-b-TPGS(2k) NPs) was prepared. The...

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Autores principales: Zhao, Tiejun, Chen, Hezhong, Dong, Yuchao, Zhang, Jiajun, Huang, Haidong, Zhu, Ji, Zhang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658437/
https://www.ncbi.nlm.nih.gov/pubmed/23696703
http://dx.doi.org/10.2147/IJN.S44220
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author Zhao, Tiejun
Chen, Hezhong
Dong, Yuchao
Zhang, Jiajun
Huang, Haidong
Zhu, Ji
Zhang, Wei
author_facet Zhao, Tiejun
Chen, Hezhong
Dong, Yuchao
Zhang, Jiajun
Huang, Haidong
Zhu, Ji
Zhang, Wei
author_sort Zhao, Tiejun
collection PubMed
description In order to improve the therapeutic efficacy and minimize the side effects of lung cancer chemotherapy, the formulation of paclitaxel-loaded poly(glycolide-co-ε-caprolactone)-b-D-α-tocopheryl polyethylene glycol 2000 succinate nanoparticles (PTX-loaded [PGA-co-PCL]-b-TPGS(2k) NPs) was prepared. The novel amphiphilic copolymer (PGA-co-PCL)-b-TPGS(2k) was synthesized by ring-opening polymerization and characterized by proton nuclear magnetic resonance spectroscopy and gel permeation chromatography. The PTX-loaded (PGA-co-PCL)-b-TPGS(2k) NPs were characterized in terms of size, size distribution, zeta potential, drug encapsulation, surface morphology, and drug release. In vitro cellular uptakes of NPs were investigated with confocal laser scanning microscopy, indicating the coumarin 6-loaded (PGA-co-PCL)-b-TPGS(2k) NPs could be internalized by human lung cancer A-549 cells. The antitumor effect of PTX-loaded NPs was evaluated, both in vitro and in vivo, on an A-549 cell tumor-bearing mouse model via intratumoral injection. The commercial PTX formulation Taxol was chosen as the reference. Experimental results showed that the PTX-loaded NPs possessed higher cytotoxicity and could effectively inhibit the growth of tumor. All the results suggested that amphiphilic copolymer (PGA-co-PCL)-b-TPGS(2k) could act as a potential biological material for nanoformulation in the treatment of lung cancer.
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spelling pubmed-36584372013-05-21 Paclitaxel-loaded poly(glycolide-co-ε-caprolactone)-b-D-α-tocopheryl polyethylene glycol 2000 succinate nanoparticles for lung cancer therapy Zhao, Tiejun Chen, Hezhong Dong, Yuchao Zhang, Jiajun Huang, Haidong Zhu, Ji Zhang, Wei Int J Nanomedicine Original Research In order to improve the therapeutic efficacy and minimize the side effects of lung cancer chemotherapy, the formulation of paclitaxel-loaded poly(glycolide-co-ε-caprolactone)-b-D-α-tocopheryl polyethylene glycol 2000 succinate nanoparticles (PTX-loaded [PGA-co-PCL]-b-TPGS(2k) NPs) was prepared. The novel amphiphilic copolymer (PGA-co-PCL)-b-TPGS(2k) was synthesized by ring-opening polymerization and characterized by proton nuclear magnetic resonance spectroscopy and gel permeation chromatography. The PTX-loaded (PGA-co-PCL)-b-TPGS(2k) NPs were characterized in terms of size, size distribution, zeta potential, drug encapsulation, surface morphology, and drug release. In vitro cellular uptakes of NPs were investigated with confocal laser scanning microscopy, indicating the coumarin 6-loaded (PGA-co-PCL)-b-TPGS(2k) NPs could be internalized by human lung cancer A-549 cells. The antitumor effect of PTX-loaded NPs was evaluated, both in vitro and in vivo, on an A-549 cell tumor-bearing mouse model via intratumoral injection. The commercial PTX formulation Taxol was chosen as the reference. Experimental results showed that the PTX-loaded NPs possessed higher cytotoxicity and could effectively inhibit the growth of tumor. All the results suggested that amphiphilic copolymer (PGA-co-PCL)-b-TPGS(2k) could act as a potential biological material for nanoformulation in the treatment of lung cancer. Dove Medical Press 2013 2013-05-16 /pmc/articles/PMC3658437/ /pubmed/23696703 http://dx.doi.org/10.2147/IJN.S44220 Text en © 2013 Zhao et al, publisher and licensee Dove Medical Press Ltd This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.
spellingShingle Original Research
Zhao, Tiejun
Chen, Hezhong
Dong, Yuchao
Zhang, Jiajun
Huang, Haidong
Zhu, Ji
Zhang, Wei
Paclitaxel-loaded poly(glycolide-co-ε-caprolactone)-b-D-α-tocopheryl polyethylene glycol 2000 succinate nanoparticles for lung cancer therapy
title Paclitaxel-loaded poly(glycolide-co-ε-caprolactone)-b-D-α-tocopheryl polyethylene glycol 2000 succinate nanoparticles for lung cancer therapy
title_full Paclitaxel-loaded poly(glycolide-co-ε-caprolactone)-b-D-α-tocopheryl polyethylene glycol 2000 succinate nanoparticles for lung cancer therapy
title_fullStr Paclitaxel-loaded poly(glycolide-co-ε-caprolactone)-b-D-α-tocopheryl polyethylene glycol 2000 succinate nanoparticles for lung cancer therapy
title_full_unstemmed Paclitaxel-loaded poly(glycolide-co-ε-caprolactone)-b-D-α-tocopheryl polyethylene glycol 2000 succinate nanoparticles for lung cancer therapy
title_short Paclitaxel-loaded poly(glycolide-co-ε-caprolactone)-b-D-α-tocopheryl polyethylene glycol 2000 succinate nanoparticles for lung cancer therapy
title_sort paclitaxel-loaded poly(glycolide-co-ε-caprolactone)-b-d-α-tocopheryl polyethylene glycol 2000 succinate nanoparticles for lung cancer therapy
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658437/
https://www.ncbi.nlm.nih.gov/pubmed/23696703
http://dx.doi.org/10.2147/IJN.S44220
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