Evaluation of Eye Irritation Potential of Aqueous Leaf Extract of Achyranthes aspera by In Vitro and In Vivo Method

The present paper is an attempt to investigate the eye irritation potential of aqueous leaf extract of Achyranthes aspera by in vitro, Hen's Egg Chorioallantoic Membrane Test (HET-CAM) and in vivo acute eye irritation test in rabbits. The irritation score (IS) obtained after treatment of the ex...

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Autores principales: Rajpal Deshmukh, Gajanan, Hema Kumar, Kuntrapakam, Suresh Reddy, Poojari Venkata, Srinivasa Rao, Boddapati, Venkata Satish Kumar, Chirumamilla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scholarly Research Network 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658502/
https://www.ncbi.nlm.nih.gov/pubmed/23724295
http://dx.doi.org/10.5402/2012/693489
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author Rajpal Deshmukh, Gajanan
Hema Kumar, Kuntrapakam
Suresh Reddy, Poojari Venkata
Srinivasa Rao, Boddapati
Venkata Satish Kumar, Chirumamilla
author_facet Rajpal Deshmukh, Gajanan
Hema Kumar, Kuntrapakam
Suresh Reddy, Poojari Venkata
Srinivasa Rao, Boddapati
Venkata Satish Kumar, Chirumamilla
author_sort Rajpal Deshmukh, Gajanan
collection PubMed
description The present paper is an attempt to investigate the eye irritation potential of aqueous leaf extract of Achyranthes aspera by in vitro, Hen's Egg Chorioallantoic Membrane Test (HET-CAM) and in vivo acute eye irritation test in rabbits. The irritation score (IS) obtained after treatment of the extract on HET-CAM is 0.07 and that of in rabbits is 0.55, Which does not comes under either category 1 or 2 as per the harmonized integrated classification system. The aqueous extract of Achyranthes aspera showed no eye irritation properties both in vitro and in vivo methods when compared with negative control whereas positive controls showed eye irritation potential.
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spelling pubmed-36585022013-05-30 Evaluation of Eye Irritation Potential of Aqueous Leaf Extract of Achyranthes aspera by In Vitro and In Vivo Method Rajpal Deshmukh, Gajanan Hema Kumar, Kuntrapakam Suresh Reddy, Poojari Venkata Srinivasa Rao, Boddapati Venkata Satish Kumar, Chirumamilla ISRN Toxicol Research Article The present paper is an attempt to investigate the eye irritation potential of aqueous leaf extract of Achyranthes aspera by in vitro, Hen's Egg Chorioallantoic Membrane Test (HET-CAM) and in vivo acute eye irritation test in rabbits. The irritation score (IS) obtained after treatment of the extract on HET-CAM is 0.07 and that of in rabbits is 0.55, Which does not comes under either category 1 or 2 as per the harmonized integrated classification system. The aqueous extract of Achyranthes aspera showed no eye irritation properties both in vitro and in vivo methods when compared with negative control whereas positive controls showed eye irritation potential. International Scholarly Research Network 2012-01-31 /pmc/articles/PMC3658502/ /pubmed/23724295 http://dx.doi.org/10.5402/2012/693489 Text en Copyright © 2012 Gajanan Rajpal Deshmukh et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Rajpal Deshmukh, Gajanan
Hema Kumar, Kuntrapakam
Suresh Reddy, Poojari Venkata
Srinivasa Rao, Boddapati
Venkata Satish Kumar, Chirumamilla
Evaluation of Eye Irritation Potential of Aqueous Leaf Extract of Achyranthes aspera by In Vitro and In Vivo Method
title Evaluation of Eye Irritation Potential of Aqueous Leaf Extract of Achyranthes aspera by In Vitro and In Vivo Method
title_full Evaluation of Eye Irritation Potential of Aqueous Leaf Extract of Achyranthes aspera by In Vitro and In Vivo Method
title_fullStr Evaluation of Eye Irritation Potential of Aqueous Leaf Extract of Achyranthes aspera by In Vitro and In Vivo Method
title_full_unstemmed Evaluation of Eye Irritation Potential of Aqueous Leaf Extract of Achyranthes aspera by In Vitro and In Vivo Method
title_short Evaluation of Eye Irritation Potential of Aqueous Leaf Extract of Achyranthes aspera by In Vitro and In Vivo Method
title_sort evaluation of eye irritation potential of aqueous leaf extract of achyranthes aspera by in vitro and in vivo method
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658502/
https://www.ncbi.nlm.nih.gov/pubmed/23724295
http://dx.doi.org/10.5402/2012/693489
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