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Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium

Salmonella Typhimurium is an important pathogen having a broad host range. In human population it causes mostly gastroenteritis but there are reports in which it was found to be responsible to cause several lethal diseases like endocarditis and meningitis. Poultry products are the major sources of t...

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Autores principales: Jha, Richa, Kumar, Anil, Saxena, Anjani, Tamuly, Shantanu, Saxena, M. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scholarly Research Network 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658560/
https://www.ncbi.nlm.nih.gov/pubmed/23762587
http://dx.doi.org/10.5402/2012/512848
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author Jha, Richa
Kumar, Anil
Saxena, Anjani
Tamuly, Shantanu
Saxena, M. K.
author_facet Jha, Richa
Kumar, Anil
Saxena, Anjani
Tamuly, Shantanu
Saxena, M. K.
author_sort Jha, Richa
collection PubMed
description Salmonella Typhimurium is an important pathogen having a broad host range. In human population it causes mostly gastroenteritis but there are reports in which it was found to be responsible to cause several lethal diseases like endocarditis and meningitis. Poultry products are the major sources of this organism in India as these are consumed at various stages of cooking. The available vaccines have their own limitations such as short-term immunity. Outer membrane proteins have shown some promising potential, so in the present study Omp C of Salmonella Typhimurium was cloned and sequenced to explore the possibility of development of r-DNA vaccine against Salmonella Typhimurium for poultry. The sequence of Omp C was studied for antigenic indexing, epitope mapping, and MHC mapping using various bioinformatic tools. The ORF analysis revealed a complete coding region of approximately 1000 bp. Five major and 13 minor B-cell epitopes were identified having an antigenic index of 1.7. The sequences also showed major histocompatibility complex (MHC) class I and class II binding region indicating a potential of eliciting cell-mediated immune response. The findings indicate that Omp C may be proven as promising candidate for development of r-DNA vaccine against Salmonella Typhimurium.
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spelling pubmed-36585602013-06-12 Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium Jha, Richa Kumar, Anil Saxena, Anjani Tamuly, Shantanu Saxena, M. K. ISRN Vet Sci Research Article Salmonella Typhimurium is an important pathogen having a broad host range. In human population it causes mostly gastroenteritis but there are reports in which it was found to be responsible to cause several lethal diseases like endocarditis and meningitis. Poultry products are the major sources of this organism in India as these are consumed at various stages of cooking. The available vaccines have their own limitations such as short-term immunity. Outer membrane proteins have shown some promising potential, so in the present study Omp C of Salmonella Typhimurium was cloned and sequenced to explore the possibility of development of r-DNA vaccine against Salmonella Typhimurium for poultry. The sequence of Omp C was studied for antigenic indexing, epitope mapping, and MHC mapping using various bioinformatic tools. The ORF analysis revealed a complete coding region of approximately 1000 bp. Five major and 13 minor B-cell epitopes were identified having an antigenic index of 1.7. The sequences also showed major histocompatibility complex (MHC) class I and class II binding region indicating a potential of eliciting cell-mediated immune response. The findings indicate that Omp C may be proven as promising candidate for development of r-DNA vaccine against Salmonella Typhimurium. International Scholarly Research Network 2012-03-05 /pmc/articles/PMC3658560/ /pubmed/23762587 http://dx.doi.org/10.5402/2012/512848 Text en Copyright © 2012 Richa Jha et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Jha, Richa
Kumar, Anil
Saxena, Anjani
Tamuly, Shantanu
Saxena, M. K.
Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium
title Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium
title_full Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium
title_fullStr Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium
title_full_unstemmed Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium
title_short Cloning, Sequencing and In Silico Analysis of Omp C of Salmonella Typhimurium
title_sort cloning, sequencing and in silico analysis of omp c of salmonella typhimurium
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658560/
https://www.ncbi.nlm.nih.gov/pubmed/23762587
http://dx.doi.org/10.5402/2012/512848
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