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Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant, Brazilian Pepper Tree

In this study, we reported thirty-nine endophytic fungi identified as Colletotrichum spp. associated with Brazilian pepper tree or aroeira (Schinus terebinthifolius Raddi. Anacardiaceae) in Paraná state, Brazil. These endophytes were identified by morphological and molecular methods, using PCR taxon...

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Autores principales: Lima, J. S., Figueiredo, J. G., Gomes, R. G., Stringari, D., Goulin, E. H., Adamoski, D., Kava-Cordeiro, V., Galli-Terasawa, L. V., Glienke, C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scholarly Research Network 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658576/
https://www.ncbi.nlm.nih.gov/pubmed/23724319
http://dx.doi.org/10.5402/2012/215716
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author Lima, J. S.
Figueiredo, J. G.
Gomes, R. G.
Stringari, D.
Goulin, E. H.
Adamoski, D.
Kava-Cordeiro, V.
Galli-Terasawa, L. V.
Glienke, C.
author_facet Lima, J. S.
Figueiredo, J. G.
Gomes, R. G.
Stringari, D.
Goulin, E. H.
Adamoski, D.
Kava-Cordeiro, V.
Galli-Terasawa, L. V.
Glienke, C.
author_sort Lima, J. S.
collection PubMed
description In this study, we reported thirty-nine endophytic fungi identified as Colletotrichum spp. associated with Brazilian pepper tree or aroeira (Schinus terebinthifolius Raddi. Anacardiaceae) in Paraná state, Brazil. These endophytes were identified by morphological and molecular methods, using PCR taxon-specific with CaInt/ITS4, CgInt/ITS4, and Col1/ITS4 primers, which amplify specific bands in C. acutatum, C. gloeosporioides lato sensu, and Colletotrichum boninensis, respectively, and by DNA sequence analysis of the nrDNA internal transcribed spacer region (ITS1, 5.8S, ITS2). We also assayed the presence of dsRNA particles in Colletotrichum spp. isolates. Combining both morphological characters and molecular data, we identified the species C. gloeosporioides, C. boninense, and C. simmondsii. However, we found a high genetic variability intraspecific in C. gloeosporioides which suggests the existence of several other species. Bands of double-stranded RNA (dsRNA) were detected in three of thirty-nine isolates. Identity of these bands was confirmed by RNAse, DNAse, and S1 nuclease treatments for the isolates LGMF633, LGMF726, and LGMF729. This is the first study reporting these particles of dsRNA in C. gloeosporioides.
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spelling pubmed-36585762013-05-30 Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant, Brazilian Pepper Tree Lima, J. S. Figueiredo, J. G. Gomes, R. G. Stringari, D. Goulin, E. H. Adamoski, D. Kava-Cordeiro, V. Galli-Terasawa, L. V. Glienke, C. ISRN Microbiol Research Article In this study, we reported thirty-nine endophytic fungi identified as Colletotrichum spp. associated with Brazilian pepper tree or aroeira (Schinus terebinthifolius Raddi. Anacardiaceae) in Paraná state, Brazil. These endophytes were identified by morphological and molecular methods, using PCR taxon-specific with CaInt/ITS4, CgInt/ITS4, and Col1/ITS4 primers, which amplify specific bands in C. acutatum, C. gloeosporioides lato sensu, and Colletotrichum boninensis, respectively, and by DNA sequence analysis of the nrDNA internal transcribed spacer region (ITS1, 5.8S, ITS2). We also assayed the presence of dsRNA particles in Colletotrichum spp. isolates. Combining both morphological characters and molecular data, we identified the species C. gloeosporioides, C. boninense, and C. simmondsii. However, we found a high genetic variability intraspecific in C. gloeosporioides which suggests the existence of several other species. Bands of double-stranded RNA (dsRNA) were detected in three of thirty-nine isolates. Identity of these bands was confirmed by RNAse, DNAse, and S1 nuclease treatments for the isolates LGMF633, LGMF726, and LGMF729. This is the first study reporting these particles of dsRNA in C. gloeosporioides. International Scholarly Research Network 2012-05-30 /pmc/articles/PMC3658576/ /pubmed/23724319 http://dx.doi.org/10.5402/2012/215716 Text en Copyright © 2012 J. S. Lima et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lima, J. S.
Figueiredo, J. G.
Gomes, R. G.
Stringari, D.
Goulin, E. H.
Adamoski, D.
Kava-Cordeiro, V.
Galli-Terasawa, L. V.
Glienke, C.
Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant, Brazilian Pepper Tree
title Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant, Brazilian Pepper Tree
title_full Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant, Brazilian Pepper Tree
title_fullStr Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant, Brazilian Pepper Tree
title_full_unstemmed Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant, Brazilian Pepper Tree
title_short Genetic Diversity of Colletotrichum spp. an Endophytic Fungi in a Medicinal Plant, Brazilian Pepper Tree
title_sort genetic diversity of colletotrichum spp. an endophytic fungi in a medicinal plant, brazilian pepper tree
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658576/
https://www.ncbi.nlm.nih.gov/pubmed/23724319
http://dx.doi.org/10.5402/2012/215716
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