Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds

By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. The...

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Detalles Bibliográficos
Autores principales: Blibech, Monia, Ellouz Ghorbel, Raoudha, Chaari, Fatma, Dammak, Ilyes, Bhiri, Fatma, Neifar, Mohamed, Ellouz Chaabouni, Semia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Scholarly Research Network 2011
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658641/
https://www.ncbi.nlm.nih.gov/pubmed/23724314
http://dx.doi.org/10.5402/2011/938347
Descripción
Sumario:By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.

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