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Anti-Quorum Sensing Potential of Crude Kigelia africana Fruit Extracts

The increasing incidence of multidrug-resistant pathogens has stimulated the search for novel anti-virulence compounds. Although many phytochemicals show promising antimicrobial activity, their power lies in their anti-virulence properties. Thus the quorum sensing (QS) inhibitory activity of four cr...

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Autor principal: Chenia, Hafizah Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658715/
https://www.ncbi.nlm.nih.gov/pubmed/23447012
http://dx.doi.org/10.3390/s130302802
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author Chenia, Hafizah Y.
author_facet Chenia, Hafizah Y.
author_sort Chenia, Hafizah Y.
collection PubMed
description The increasing incidence of multidrug-resistant pathogens has stimulated the search for novel anti-virulence compounds. Although many phytochemicals show promising antimicrobial activity, their power lies in their anti-virulence properties. Thus the quorum sensing (QS) inhibitory activity of four crude Kigelia africana fruit extracts was assessed qualitatively and quantitatively using the Chromobacterium violaceum and Agrobacterium tumefaciens biosensor systems. Inhibition of QS-controlled violacein production in C. violaceum was assayed using the qualitative agar diffusion assay as well as by quantifying violacein inhibition using K. africana extracts ranging from 0.31–8.2 mg/mL. Qualitative modulation of QS activity was investigated using the agar diffusion double ring assay. All four extracts showed varying levels of anti-QS activity with zones of violacein inhibition ranging from 9–10 mm. The effect on violacein inhibition was significant in the following order: hexane > dichloromethane > ethyl acetate > methanol. Inhibition was concentration-dependent, with the ≥90% inhibition being obtained with ≥1.3 mg/mL of the hexane extract. Both LuxI and LuxR activity were affected by crude extracts suggesting that the phytochemicals target both QS signal and receptor. K. africana extracts with their anti-QS activity, have the potential to be novel therapeutic agents, which might be important in reducing virulence and pathogenicity of drug-resistant bacteria in vivo.
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spelling pubmed-36587152013-05-30 Anti-Quorum Sensing Potential of Crude Kigelia africana Fruit Extracts Chenia, Hafizah Y. Sensors (Basel) Article The increasing incidence of multidrug-resistant pathogens has stimulated the search for novel anti-virulence compounds. Although many phytochemicals show promising antimicrobial activity, their power lies in their anti-virulence properties. Thus the quorum sensing (QS) inhibitory activity of four crude Kigelia africana fruit extracts was assessed qualitatively and quantitatively using the Chromobacterium violaceum and Agrobacterium tumefaciens biosensor systems. Inhibition of QS-controlled violacein production in C. violaceum was assayed using the qualitative agar diffusion assay as well as by quantifying violacein inhibition using K. africana extracts ranging from 0.31–8.2 mg/mL. Qualitative modulation of QS activity was investigated using the agar diffusion double ring assay. All four extracts showed varying levels of anti-QS activity with zones of violacein inhibition ranging from 9–10 mm. The effect on violacein inhibition was significant in the following order: hexane > dichloromethane > ethyl acetate > methanol. Inhibition was concentration-dependent, with the ≥90% inhibition being obtained with ≥1.3 mg/mL of the hexane extract. Both LuxI and LuxR activity were affected by crude extracts suggesting that the phytochemicals target both QS signal and receptor. K. africana extracts with their anti-QS activity, have the potential to be novel therapeutic agents, which might be important in reducing virulence and pathogenicity of drug-resistant bacteria in vivo. Molecular Diversity Preservation International (MDPI) 2013-02-27 /pmc/articles/PMC3658715/ /pubmed/23447012 http://dx.doi.org/10.3390/s130302802 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Chenia, Hafizah Y.
Anti-Quorum Sensing Potential of Crude Kigelia africana Fruit Extracts
title Anti-Quorum Sensing Potential of Crude Kigelia africana Fruit Extracts
title_full Anti-Quorum Sensing Potential of Crude Kigelia africana Fruit Extracts
title_fullStr Anti-Quorum Sensing Potential of Crude Kigelia africana Fruit Extracts
title_full_unstemmed Anti-Quorum Sensing Potential of Crude Kigelia africana Fruit Extracts
title_short Anti-Quorum Sensing Potential of Crude Kigelia africana Fruit Extracts
title_sort anti-quorum sensing potential of crude kigelia africana fruit extracts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658715/
https://www.ncbi.nlm.nih.gov/pubmed/23447012
http://dx.doi.org/10.3390/s130302802
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