Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers

We have developed a fluorescence-based fiber-optical biosensor, which can selectively detect different antibodies in serial at preselected positions inside a single piece of fiber. The fiber is a microstructured polymer optical fiber fabricated from TOPAS cyclic olefin copolymer, which allows for UV...

Descripción completa

Detalles Bibliográficos
Autores principales: Emiliyanov, Grigoriy, Høiby, Poul E., Pedersen, Lars H., Bang, Ole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658743/
https://www.ncbi.nlm.nih.gov/pubmed/23529122
http://dx.doi.org/10.3390/s130303242
Descripción
Sumario:We have developed a fluorescence-based fiber-optical biosensor, which can selectively detect different antibodies in serial at preselected positions inside a single piece of fiber. The fiber is a microstructured polymer optical fiber fabricated from TOPAS cyclic olefin copolymer, which allows for UV activation of localized sensor layers inside the holes of the fiber. Serial fluorescence-based selective sensing of Cy3-labelled α-streptavidin and Cy5-labelled α-CRP antibodies is demonstrated.

Ejemplares similares