Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers

We have developed a fluorescence-based fiber-optical biosensor, which can selectively detect different antibodies in serial at preselected positions inside a single piece of fiber. The fiber is a microstructured polymer optical fiber fabricated from TOPAS cyclic olefin copolymer, which allows for UV...

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Detalles Bibliográficos
Autores principales: Emiliyanov, Grigoriy, Høiby, Poul E., Pedersen, Lars H., Bang, Ole
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658743/
https://www.ncbi.nlm.nih.gov/pubmed/23529122
http://dx.doi.org/10.3390/s130303242
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author Emiliyanov, Grigoriy
Høiby, Poul E.
Pedersen, Lars H.
Bang, Ole
author_facet Emiliyanov, Grigoriy
Høiby, Poul E.
Pedersen, Lars H.
Bang, Ole
author_sort Emiliyanov, Grigoriy
collection PubMed
description We have developed a fluorescence-based fiber-optical biosensor, which can selectively detect different antibodies in serial at preselected positions inside a single piece of fiber. The fiber is a microstructured polymer optical fiber fabricated from TOPAS cyclic olefin copolymer, which allows for UV activation of localized sensor layers inside the holes of the fiber. Serial fluorescence-based selective sensing of Cy3-labelled α-streptavidin and Cy5-labelled α-CRP antibodies is demonstrated.
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spelling pubmed-36587432013-05-30 Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers Emiliyanov, Grigoriy Høiby, Poul E. Pedersen, Lars H. Bang, Ole Sensors (Basel) Article We have developed a fluorescence-based fiber-optical biosensor, which can selectively detect different antibodies in serial at preselected positions inside a single piece of fiber. The fiber is a microstructured polymer optical fiber fabricated from TOPAS cyclic olefin copolymer, which allows for UV activation of localized sensor layers inside the holes of the fiber. Serial fluorescence-based selective sensing of Cy3-labelled α-streptavidin and Cy5-labelled α-CRP antibodies is demonstrated. Molecular Diversity Preservation International (MDPI) 2013-03-08 /pmc/articles/PMC3658743/ /pubmed/23529122 http://dx.doi.org/10.3390/s130303242 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Emiliyanov, Grigoriy
Høiby, Poul E.
Pedersen, Lars H.
Bang, Ole
Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers
title Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers
title_full Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers
title_fullStr Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers
title_full_unstemmed Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers
title_short Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers
title_sort selective serial multi-antibody biosensing with topas microstructured polymer optical fibers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658743/
https://www.ncbi.nlm.nih.gov/pubmed/23529122
http://dx.doi.org/10.3390/s130303242
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