Cargando…

Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum

BACKGROUND: Reducing the production cost of, and increasing revenues from, industrial biofuels will greatly facilitate their proliferation and co-integration with fossil fuels. The cost of feedstock is the largest cost in most fermentation bioprocesses and therefore represents an important target fo...

Descripción completa

Detalles Bibliográficos
Autores principales:	Pyne, Michael E, Moo-Young, Murray, Chung, Duane A, Chou, C Perry
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2013
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3658993/ https://www.ncbi.nlm.nih.gov/pubmed/23570573 http://dx.doi.org/10.1186/1754-6834-6-50

Ejemplares similares

Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme
por: Pyne, Michael E, et al.
Publicado: (2014)

Genome-directed analysis of prophage excision, host defence systems, and central fermentative metabolism in Clostridium pasteurianum
por: Pyne, Michael E., et al.
Publicado: (2016)

Improved Draft Genome Sequence of Clostridium pasteurianum Strain ATCC 6013 (DSM 525) Using a Hybrid Next-Generation Sequencing Approach
por: Pyne, Michael E., et al.
Publicado: (2014)

Dam and Dcm methylations prevent gene transfer into Clostridium pasteurianum NRRL B-598: development of methods for electrotransformation, conjugation, and sonoporation
por: Kolek, Jan, et al.
Publicado: (2016)

An optimized electrotransformation protocol for Lactobacillus jensenii
por: Fristot, Elsa, et al.
Publicado: (2023)

Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in Clostridium
por: Pyne, Michael E., et al.
Publicado: (2016)

Closed Genome Sequence of Clostridium pasteurianum ATCC 6013
por: Rotta, Carlo, et al.
Publicado: (2015)

Towards improved butanol production through targeted genetic modification of Clostridium pasteurianum
por: Schwarz, Katrin M., et al.
Publicado: (2017)

Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
por: Sabra, Wael, et al.
Publicado: (2016)

Improving gene transfer in Clostridium pasteurianum through the isolation of rare hypertransformable variants
por: Grosse-Honebrink, Alexander, et al.
Publicado: (2017)

Metabolomic and kinetic investigations on the electricity‐aided production of butanol by Clostridium pasteurianum strains
por: Arbter, Philipp, et al.
Publicado: (2020)

The optimization system for preparation of TG1 competent cells and electrotransformation
por: Chai, Dafei, et al.
Publicado: (2020)

Complete Genome Sequence of the Nitrogen-Fixing and Solvent-Producing Clostridium pasteurianum DSM 525
por: Poehlein, Anja, et al.
Publicado: (2015)

Changes in Membrane Plasmalogens of Clostridium pasteurianum during Butanol Fermentation as Determined by Lipidomic Analysis
por: Kolek, Jan, et al.
Publicado: (2015)

Cooperative growth of Geobacter sulfurreducens and Clostridium pasteurianum with subsequent metabolic shift in glycerol fermentation
por: Moscoviz, Roman, et al.
Publicado: (2017)

Phenotype analysis of cultivation processes via unsupervised machine learning: Demonstration for Clostridium pasteurianum
por: Hong, Yaeseong, et al.
Publicado: (2021)

Electricity-driven metabolic shift through direct electron uptake by electroactive heterotroph Clostridium pasteurianum
por: Choi, Okkyoung, et al.
Publicado: (2014)

Draft Genome Sequence of Clostridium pasteurianum NRRL B-598, a Potential Butanol or Hydrogen Producer
por: Kolek, Jan, et al.
Publicado: (2014)

Influence of Processing Parameters and Natural Antimicrobial on Alicyclobacillus acidoterrestris and Clostridium pasteurianum Using Response Surface Methodology
por: Hadj Saadoun, Jasmine, et al.
Publicado: (2022)

Deficiency of exopolysaccharides and O-antigen makes Halomonas bluephagenesis self-flocculating and amenable to electrotransformation
por: Xu, Tong, et al.
Publicado: (2022)

Improved n-butanol production by a non-acetone producing Clostridium pasteurianum DSMZ 525 in mixed substrate fermentation
por: Sabra, Wael, et al.
Publicado: (2014)

Production of 1,3-PDO and butanol by a mutant strain of Clostridium pasteurianum with increased tolerance towards crude glycerol
por: Jensen, Torbjørn Ølshøj, et al.
Publicado: (2012)

Metabolic and proteomic analyses of product selectivity and redox regulation in Clostridium pasteurianum grown on glycerol under varied iron availability
por: Groeger, Christin, et al.
Publicado: (2017)

Increased Butanol Yields through Cosubstrate Fermentation of Jerusalem Artichoke Tubers and Crude Glycerol by Clostridium pasteurianum DSM 525
por: Sarchami, Tahereh, et al.
Publicado: (2019)

High efficiency electrotransformation of Lactococcus lactis spp. lactis cells pretreated with lithium acetate and dithiothreitol
por: Papagianni, Maria, et al.
Publicado: (2007)

Draft Genome Sequence of Type Strain Clostridium pasteurianum DSM 525 (ATCC 6013), a Promising Producer of Chemicals and Fuels
por: Rappert, Sugima, et al.
Publicado: (2013)

Hyperfine-Shifted (13)C and (15)N NMR Signals from Clostridium pasteurianum Rubredoxin: Extensive Assignments and Quantum Chemical Verification
por: Lin, I-Jin, et al.
Publicado: (2009)

Nitrogenase MoFe protein from Clostridium pasteurianum at 1.08 Å resolution: comparison with the Azotobacter vinelandii MoFe protein
por: Zhang, Li-Mei, et al.
Publicado: (2015)

Control of redox potential in a novel continuous bioelectrochemical system led to remarkable metabolic and energetic responses of Clostridium pasteurianum grown on glycerol
por: Arbter, Philipp, et al.
Publicado: (2022)

The Physiological Functions and Structural Determinants of Catalytic Bias in the [FeFe]-Hydrogenases CpI and CpII of Clostridium pasteurianum Strain W5
por: Therien, Jesse B., et al.
Publicado: (2017)

Whole-genome sequence of an evolved Clostridium pasteurianum strain reveals Spo0A deficiency responsible for increased butanol production and superior growth
por: Sandoval, Nicholas R., et al.
Publicado: (2015)

Bio-butanol production from glycerol with Clostridium pasteurianum CH4: the effects of butyrate addition and in situ butanol removal via membrane distillation
por: Lin, De-Shun, et al.
Publicado: (2015)

A novel downstream process for highly pure 1,3‐propanediol from an efficient fed‐batch fermentation of raw glycerol by Clostridium pasteurianum
por: Zhang, Chijian, et al.
Publicado: (2021)

Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli
por: Srirangan, Kajan, et al.
Publicado: (2013)

Enhancing 1,3-Propanediol Productivity in the Non-Model Chassis Clostridium beijerinckii through Genetic Manipulation
por: Bortolucci, Jonatã, et al.
Publicado: (2023)

Promiscuous plasmid replication in thermophiles: Use of a novel hyperthermophilic replicon for genetic manipulation of Clostridium thermocellum at its optimum growth temperature
por: Groom, Joseph, et al.
Publicado: (2016)

Development of Genetic Tools for the Manipulation of the Planctomycetes
por: Rivas-Marín, Elena, et al.
Publicado: (2016)

Development of methods for the genetic manipulation of Flavobacterium columnare
por: Staroscik, Andrew M, et al.
Publicado: (2008)

Genetic manipulation of betta fish
por: Palmiotti, Alec, et al.
Publicado: (2023)

Genetic Manipulation in Mucorales and New Developments to Study Mucormycosis
por: Lax, Carlos, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_ngmine5n5lih5hlcj0t45ucl7k