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Gene Expression Changes in the Colon Epithelium Are Similar to Those of Intact Colon during Late Inflammation in Interleukin-10 Gene Deficient Mice

In addition to their role in absorption and secretion, epithelial cells play an important role in the protection of the colon mucosa from the resident microbiota and are important for the maintenance of homeostasis. Microarray analysis of intact colon samples is widely used to gain an overview of th...

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Autores principales: Russ, Anna E., Peters, Jason S., McNabb, Warren C., Barnett, Matthew P. G., Anderson, Rachel C., Park, Zaneta, Zhu, Shuotun, Maclean, Paul, Young, Wayne, Reynolds, Gordon W., Roy, Nicole C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659096/
https://www.ncbi.nlm.nih.gov/pubmed/23700416
http://dx.doi.org/10.1371/journal.pone.0063251
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author Russ, Anna E.
Peters, Jason S.
McNabb, Warren C.
Barnett, Matthew P. G.
Anderson, Rachel C.
Park, Zaneta
Zhu, Shuotun
Maclean, Paul
Young, Wayne
Reynolds, Gordon W.
Roy, Nicole C.
author_facet Russ, Anna E.
Peters, Jason S.
McNabb, Warren C.
Barnett, Matthew P. G.
Anderson, Rachel C.
Park, Zaneta
Zhu, Shuotun
Maclean, Paul
Young, Wayne
Reynolds, Gordon W.
Roy, Nicole C.
author_sort Russ, Anna E.
collection PubMed
description In addition to their role in absorption and secretion, epithelial cells play an important role in the protection of the colon mucosa from the resident microbiota and are important for the maintenance of homeostasis. Microarray analysis of intact colon samples is widely used to gain an overview of the cellular pathways and processes that are active in the colon during inflammation. Laser microdissection of colon epithelial cells allows a more targeted analysis of molecular pathways in the mucosa, preceding and during inflammation, with potentially increased sensitivity to changes in specific cell populations. The aim of this study was to investigate the molecular changes that occur in early and late inflammation stages in colon epithelium of a mouse model of inflammatory bowel diseases. Microarray analysis of intact colon samples and microdissected colon epithelial cell samples from interleukin-10 gene deficient and control mice at 6 and 12 weeks of age was undertaken. Results of gene set enrichment analysis showed that more immune-related pathways were identified between interleukin-10 gene deficient and control mice at 6 weeks of age in epithelial cells than intact colon. This suggests that targeting epithelial cells could increase sensitivity for detecting immune changes that occur early in the inflammatory process. However, in the later stages of inflammation, microarray analyses of intact colon and epithelium both provide a similar overview of gene expression changes in the colon mucosa at the pathway level.
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spelling pubmed-36590962013-05-22 Gene Expression Changes in the Colon Epithelium Are Similar to Those of Intact Colon during Late Inflammation in Interleukin-10 Gene Deficient Mice Russ, Anna E. Peters, Jason S. McNabb, Warren C. Barnett, Matthew P. G. Anderson, Rachel C. Park, Zaneta Zhu, Shuotun Maclean, Paul Young, Wayne Reynolds, Gordon W. Roy, Nicole C. PLoS One Research Article In addition to their role in absorption and secretion, epithelial cells play an important role in the protection of the colon mucosa from the resident microbiota and are important for the maintenance of homeostasis. Microarray analysis of intact colon samples is widely used to gain an overview of the cellular pathways and processes that are active in the colon during inflammation. Laser microdissection of colon epithelial cells allows a more targeted analysis of molecular pathways in the mucosa, preceding and during inflammation, with potentially increased sensitivity to changes in specific cell populations. The aim of this study was to investigate the molecular changes that occur in early and late inflammation stages in colon epithelium of a mouse model of inflammatory bowel diseases. Microarray analysis of intact colon samples and microdissected colon epithelial cell samples from interleukin-10 gene deficient and control mice at 6 and 12 weeks of age was undertaken. Results of gene set enrichment analysis showed that more immune-related pathways were identified between interleukin-10 gene deficient and control mice at 6 weeks of age in epithelial cells than intact colon. This suggests that targeting epithelial cells could increase sensitivity for detecting immune changes that occur early in the inflammatory process. However, in the later stages of inflammation, microarray analyses of intact colon and epithelium both provide a similar overview of gene expression changes in the colon mucosa at the pathway level. Public Library of Science 2013-05-20 /pmc/articles/PMC3659096/ /pubmed/23700416 http://dx.doi.org/10.1371/journal.pone.0063251 Text en © 2013 Russ et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Russ, Anna E.
Peters, Jason S.
McNabb, Warren C.
Barnett, Matthew P. G.
Anderson, Rachel C.
Park, Zaneta
Zhu, Shuotun
Maclean, Paul
Young, Wayne
Reynolds, Gordon W.
Roy, Nicole C.
Gene Expression Changes in the Colon Epithelium Are Similar to Those of Intact Colon during Late Inflammation in Interleukin-10 Gene Deficient Mice
title Gene Expression Changes in the Colon Epithelium Are Similar to Those of Intact Colon during Late Inflammation in Interleukin-10 Gene Deficient Mice
title_full Gene Expression Changes in the Colon Epithelium Are Similar to Those of Intact Colon during Late Inflammation in Interleukin-10 Gene Deficient Mice
title_fullStr Gene Expression Changes in the Colon Epithelium Are Similar to Those of Intact Colon during Late Inflammation in Interleukin-10 Gene Deficient Mice
title_full_unstemmed Gene Expression Changes in the Colon Epithelium Are Similar to Those of Intact Colon during Late Inflammation in Interleukin-10 Gene Deficient Mice
title_short Gene Expression Changes in the Colon Epithelium Are Similar to Those of Intact Colon during Late Inflammation in Interleukin-10 Gene Deficient Mice
title_sort gene expression changes in the colon epithelium are similar to those of intact colon during late inflammation in interleukin-10 gene deficient mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659096/
https://www.ncbi.nlm.nih.gov/pubmed/23700416
http://dx.doi.org/10.1371/journal.pone.0063251
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