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Anti-Cancer Effect of Ginsenoside F(2) against Glioblastoma Multiforme in Xenograft Model in SD Rats
The glioblastoma multiforme (GBM) is the most common malignant brain tumor in adults. Despite combination treatments of radiation and chemotherapy, the survival periods are very short. Therefore, this study was conducted to assess the potential of ginsenoside F(2) (F2) to treat GBM. In in vitro expe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Ginseng
2012
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659572/ https://www.ncbi.nlm.nih.gov/pubmed/23717108 http://dx.doi.org/10.5142/jgr.2012.36.1.86 |
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author | Shin, Ji Yon Lee, Jung Min Shin, Heon Sub Park, Sang Yong Yang, Jung Eun Cho, Somi Kim Yi, Tae-Hoo |
author_facet | Shin, Ji Yon Lee, Jung Min Shin, Heon Sub Park, Sang Yong Yang, Jung Eun Cho, Somi Kim Yi, Tae-Hoo |
author_sort | Shin, Ji Yon |
collection | PubMed |
description | The glioblastoma multiforme (GBM) is the most common malignant brain tumor in adults. Despite combination treatments of radiation and chemotherapy, the survival periods are very short. Therefore, this study was conducted to assess the potential of ginsenoside F(2) (F2) to treat GBM. In in vitro experiments with glioblastoma cells U373MG, F2 showed the cytotoxic effect with IC(50) of 50 μg/mL through apoptosis, confirmed by DNA condensation and fragmentation. The cell population of cell cycle sub-G1 as indicative of apoptosis was also increased. In xenograft model in SD rats, F2 at dosage of 35 mg/kg weight was intravenously injected every two days. This reduced the tumor growth in magnetic resonance imaging images. The immunohistochemistry revealed that the anticancer activity might be mediated through inhibition of proliferation judged by Ki67 and apoptosis induced by activation of caspase-3 and -8. And the lowered expression of CD31 showed the reduction in blood vessel densities. The expression of matrix metalloproteinase-9 for invasion of cancer was also inhibited. The cell populations with cancer stem cell markers of CD133 and nestin were reduced. The results of this study suggested that F2 could be a new potential chemotherapeutic drug for GBM treatment by inhibiting the growth and invasion of cancer. |
format | Online Article Text |
id | pubmed-3659572 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Korean Society of Ginseng |
record_format | MEDLINE/PubMed |
spelling | pubmed-36595722013-05-28 Anti-Cancer Effect of Ginsenoside F(2) against Glioblastoma Multiforme in Xenograft Model in SD Rats Shin, Ji Yon Lee, Jung Min Shin, Heon Sub Park, Sang Yong Yang, Jung Eun Cho, Somi Kim Yi, Tae-Hoo J Ginseng Res Articles The glioblastoma multiforme (GBM) is the most common malignant brain tumor in adults. Despite combination treatments of radiation and chemotherapy, the survival periods are very short. Therefore, this study was conducted to assess the potential of ginsenoside F(2) (F2) to treat GBM. In in vitro experiments with glioblastoma cells U373MG, F2 showed the cytotoxic effect with IC(50) of 50 μg/mL through apoptosis, confirmed by DNA condensation and fragmentation. The cell population of cell cycle sub-G1 as indicative of apoptosis was also increased. In xenograft model in SD rats, F2 at dosage of 35 mg/kg weight was intravenously injected every two days. This reduced the tumor growth in magnetic resonance imaging images. The immunohistochemistry revealed that the anticancer activity might be mediated through inhibition of proliferation judged by Ki67 and apoptosis induced by activation of caspase-3 and -8. And the lowered expression of CD31 showed the reduction in blood vessel densities. The expression of matrix metalloproteinase-9 for invasion of cancer was also inhibited. The cell populations with cancer stem cell markers of CD133 and nestin were reduced. The results of this study suggested that F2 could be a new potential chemotherapeutic drug for GBM treatment by inhibiting the growth and invasion of cancer. The Korean Society of Ginseng 2012-01 /pmc/articles/PMC3659572/ /pubmed/23717108 http://dx.doi.org/10.5142/jgr.2012.36.1.86 Text en Copyright ©2012, The Korean Society of Ginseng http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Shin, Ji Yon Lee, Jung Min Shin, Heon Sub Park, Sang Yong Yang, Jung Eun Cho, Somi Kim Yi, Tae-Hoo Anti-Cancer Effect of Ginsenoside F(2) against Glioblastoma Multiforme in Xenograft Model in SD Rats |
title | Anti-Cancer Effect of Ginsenoside F(2) against Glioblastoma Multiforme in Xenograft Model in SD Rats |
title_full | Anti-Cancer Effect of Ginsenoside F(2) against Glioblastoma Multiforme in Xenograft Model in SD Rats |
title_fullStr | Anti-Cancer Effect of Ginsenoside F(2) against Glioblastoma Multiforme in Xenograft Model in SD Rats |
title_full_unstemmed | Anti-Cancer Effect of Ginsenoside F(2) against Glioblastoma Multiforme in Xenograft Model in SD Rats |
title_short | Anti-Cancer Effect of Ginsenoside F(2) against Glioblastoma Multiforme in Xenograft Model in SD Rats |
title_sort | anti-cancer effect of ginsenoside f(2) against glioblastoma multiforme in xenograft model in sd rats |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659572/ https://www.ncbi.nlm.nih.gov/pubmed/23717108 http://dx.doi.org/10.5142/jgr.2012.36.1.86 |
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