Cargando…

Processed Panax ginseng, Sun Ginseng, Decreases Oxidative Damage Induced by tert-butyl Hydroperoxide via Regulation of Antioxidant Enzyme and Anti-apoptotic Molecules in HepG2 Cells

Potential antioxidant effect of processed ginseng (sun ginseng, SG) on oxidative stress generated by tert-butyl hydroperoxide (t-BHP) was investigated in HepG2 cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and lactate dehydrogenase (LDH) leakage test demonstrated that SG...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Hyejin, Kim, Jinhee, Lee, Seo Young, Park, Jeong Hill, Hwang, Gwi Seo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Ginseng 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659594/
https://www.ncbi.nlm.nih.gov/pubmed/23717125
http://dx.doi.org/10.5142/jgr.2012.36.3.248
_version_ 1782270469620105216
author Lee, Hyejin
Kim, Jinhee
Lee, Seo Young
Park, Jeong Hill
Hwang, Gwi Seo
author_facet Lee, Hyejin
Kim, Jinhee
Lee, Seo Young
Park, Jeong Hill
Hwang, Gwi Seo
author_sort Lee, Hyejin
collection PubMed
description Potential antioxidant effect of processed ginseng (sun ginseng, SG) on oxidative stress generated by tert-butyl hydroperoxide (t-BHP) was investigated in HepG2 cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and lactate dehydrogenase (LDH) leakage test demonstrated that SG dose-dependently prevents a loss of cell viability against t-BHP-induced oxidative stress. Also, SG treatment dose-dependently relieved the increment of activities of hepatic enzymes, such as aspartate aminotrasferase and alanine aminotransferase, and lipid peroxidation mediated by t-BHP treatment in HepG2 cells. SG increased the gene expression of antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase. However, high dose of SG treatment caused decrease in mRNA level of glutathione peroxidase as compared to low dosage of SG-treated cells. The gene expression of glutathione reductase was found to be slightly increased by SG treatment. In addition, SG extract attributed its hepaprotective effect by inducing the mRNA level of bcl-2 and bcl-xL but reducing that of bax. But, the gene expression of bad showed no significant change in SG-treated HepG2 cells. These findings suggest that SG has hepatoprotective effect by showing reduction of LDH release, activities of hepatic enzymes and lipid peroxidation and regulating the gene expression of antioxidant enzymes and apoptosis-related molecules against oxdative stress caused by t-BHP in HepG2 cells.
format Online
Article
Text
id pubmed-3659594
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher The Korean Society of Ginseng
record_format MEDLINE/PubMed
spelling pubmed-36595942013-05-28 Processed Panax ginseng, Sun Ginseng, Decreases Oxidative Damage Induced by tert-butyl Hydroperoxide via Regulation of Antioxidant Enzyme and Anti-apoptotic Molecules in HepG2 Cells Lee, Hyejin Kim, Jinhee Lee, Seo Young Park, Jeong Hill Hwang, Gwi Seo J Ginseng Res Articles Potential antioxidant effect of processed ginseng (sun ginseng, SG) on oxidative stress generated by tert-butyl hydroperoxide (t-BHP) was investigated in HepG2 cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and lactate dehydrogenase (LDH) leakage test demonstrated that SG dose-dependently prevents a loss of cell viability against t-BHP-induced oxidative stress. Also, SG treatment dose-dependently relieved the increment of activities of hepatic enzymes, such as aspartate aminotrasferase and alanine aminotransferase, and lipid peroxidation mediated by t-BHP treatment in HepG2 cells. SG increased the gene expression of antioxidant enzymes such as superoxide dismutase, catalase, and glutathione peroxidase. However, high dose of SG treatment caused decrease in mRNA level of glutathione peroxidase as compared to low dosage of SG-treated cells. The gene expression of glutathione reductase was found to be slightly increased by SG treatment. In addition, SG extract attributed its hepaprotective effect by inducing the mRNA level of bcl-2 and bcl-xL but reducing that of bax. But, the gene expression of bad showed no significant change in SG-treated HepG2 cells. These findings suggest that SG has hepatoprotective effect by showing reduction of LDH release, activities of hepatic enzymes and lipid peroxidation and regulating the gene expression of antioxidant enzymes and apoptosis-related molecules against oxdative stress caused by t-BHP in HepG2 cells. The Korean Society of Ginseng 2012-07 /pmc/articles/PMC3659594/ /pubmed/23717125 http://dx.doi.org/10.5142/jgr.2012.36.3.248 Text en Copyright ©2012, The Korean Society of Ginseng http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Articles
Lee, Hyejin
Kim, Jinhee
Lee, Seo Young
Park, Jeong Hill
Hwang, Gwi Seo
Processed Panax ginseng, Sun Ginseng, Decreases Oxidative Damage Induced by tert-butyl Hydroperoxide via Regulation of Antioxidant Enzyme and Anti-apoptotic Molecules in HepG2 Cells
title Processed Panax ginseng, Sun Ginseng, Decreases Oxidative Damage Induced by tert-butyl Hydroperoxide via Regulation of Antioxidant Enzyme and Anti-apoptotic Molecules in HepG2 Cells
title_full Processed Panax ginseng, Sun Ginseng, Decreases Oxidative Damage Induced by tert-butyl Hydroperoxide via Regulation of Antioxidant Enzyme and Anti-apoptotic Molecules in HepG2 Cells
title_fullStr Processed Panax ginseng, Sun Ginseng, Decreases Oxidative Damage Induced by tert-butyl Hydroperoxide via Regulation of Antioxidant Enzyme and Anti-apoptotic Molecules in HepG2 Cells
title_full_unstemmed Processed Panax ginseng, Sun Ginseng, Decreases Oxidative Damage Induced by tert-butyl Hydroperoxide via Regulation of Antioxidant Enzyme and Anti-apoptotic Molecules in HepG2 Cells
title_short Processed Panax ginseng, Sun Ginseng, Decreases Oxidative Damage Induced by tert-butyl Hydroperoxide via Regulation of Antioxidant Enzyme and Anti-apoptotic Molecules in HepG2 Cells
title_sort processed panax ginseng, sun ginseng, decreases oxidative damage induced by tert-butyl hydroperoxide via regulation of antioxidant enzyme and anti-apoptotic molecules in hepg2 cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3659594/
https://www.ncbi.nlm.nih.gov/pubmed/23717125
http://dx.doi.org/10.5142/jgr.2012.36.3.248
work_keys_str_mv AT leehyejin processedpanaxginsengsunginsengdecreasesoxidativedamageinducedbytertbutylhydroperoxideviaregulationofantioxidantenzymeandantiapoptoticmoleculesinhepg2cells
AT kimjinhee processedpanaxginsengsunginsengdecreasesoxidativedamageinducedbytertbutylhydroperoxideviaregulationofantioxidantenzymeandantiapoptoticmoleculesinhepg2cells
AT leeseoyoung processedpanaxginsengsunginsengdecreasesoxidativedamageinducedbytertbutylhydroperoxideviaregulationofantioxidantenzymeandantiapoptoticmoleculesinhepg2cells
AT parkjeonghill processedpanaxginsengsunginsengdecreasesoxidativedamageinducedbytertbutylhydroperoxideviaregulationofantioxidantenzymeandantiapoptoticmoleculesinhepg2cells
AT hwanggwiseo processedpanaxginsengsunginsengdecreasesoxidativedamageinducedbytertbutylhydroperoxideviaregulationofantioxidantenzymeandantiapoptoticmoleculesinhepg2cells