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Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster
When obtaining samples for population genetic studies, it is essential that the sampling is random. For Drosophila, one of the crucial steps in sampling experimental flies is the collection of eggs. Here an egg collection method is presented, which randomizes the eggs in a water column and diminishe...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Landes Bioscience
2013
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660287/ https://www.ncbi.nlm.nih.gov/pubmed/23247611 http://dx.doi.org/10.4161/fly.22758 |
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author | Fristrup Schou, Mads |
author_facet | Fristrup Schou, Mads |
author_sort | Fristrup Schou, Mads |
collection | PubMed |
description | When obtaining samples for population genetic studies, it is essential that the sampling is random. For Drosophila, one of the crucial steps in sampling experimental flies is the collection of eggs. Here an egg collection method is presented, which randomizes the eggs in a water column and diminishes environmental variance. This method was compared with a traditional egg collection method where eggs are collected directly from the medium. Within each method the observed and expected standard deviations of egg-to-adult viability were compared, whereby the difference in the randomness of the samples between the two methods was assessed. The method presented here was superior to the traditional method. Only 14% of the samples had a standard deviation higher than expected, as compared with 58% in the traditional method. To reduce bias in the estimation of the variance and the mean of a trait and to obtain a representative collection of genotypes, the method presented here is strongly recommended when collecting eggs from Drosophila. |
format | Online Article Text |
id | pubmed-3660287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Landes Bioscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-36602872013-06-27 Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster Fristrup Schou, Mads Fly (Austin) Methods and Technical Advances When obtaining samples for population genetic studies, it is essential that the sampling is random. For Drosophila, one of the crucial steps in sampling experimental flies is the collection of eggs. Here an egg collection method is presented, which randomizes the eggs in a water column and diminishes environmental variance. This method was compared with a traditional egg collection method where eggs are collected directly from the medium. Within each method the observed and expected standard deviations of egg-to-adult viability were compared, whereby the difference in the randomness of the samples between the two methods was assessed. The method presented here was superior to the traditional method. Only 14% of the samples had a standard deviation higher than expected, as compared with 58% in the traditional method. To reduce bias in the estimation of the variance and the mean of a trait and to obtain a representative collection of genotypes, the method presented here is strongly recommended when collecting eggs from Drosophila. Landes Bioscience 2013-01-01 2012-12-17 /pmc/articles/PMC3660287/ /pubmed/23247611 http://dx.doi.org/10.4161/fly.22758 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Methods and Technical Advances Fristrup Schou, Mads Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster |
title | Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster |
title_full | Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster |
title_fullStr | Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster |
title_full_unstemmed | Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster |
title_short | Fast egg collection method greatly improves randomness of egg sampling in Drosophila melanogaster |
title_sort | fast egg collection method greatly improves randomness of egg sampling in drosophila melanogaster |
topic | Methods and Technical Advances |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660287/ https://www.ncbi.nlm.nih.gov/pubmed/23247611 http://dx.doi.org/10.4161/fly.22758 |
work_keys_str_mv | AT fristrupschoumads fasteggcollectionmethodgreatlyimprovesrandomnessofeggsamplingindrosophilamelanogaster |