Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid

To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic...

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Detalles Bibliográficos
Autores principales: Kaihatsu, Kunihiro, Sawada, Shinjiro, Nakamura, Shota, Nakaya, Takaaki, Yasunaga, Teruo, Kato, Nobuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660326/
https://www.ncbi.nlm.nih.gov/pubmed/23704970
http://dx.doi.org/10.1371/journal.pone.0064017
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author Kaihatsu, Kunihiro
Sawada, Shinjiro
Nakamura, Shota
Nakaya, Takaaki
Yasunaga, Teruo
Kato, Nobuo
author_facet Kaihatsu, Kunihiro
Sawada, Shinjiro
Nakamura, Shota
Nakaya, Takaaki
Yasunaga, Teruo
Kato, Nobuo
author_sort Kaihatsu, Kunihiro
collection PubMed
description To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal.
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spelling pubmed-36603262013-05-23 Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid Kaihatsu, Kunihiro Sawada, Shinjiro Nakamura, Shota Nakaya, Takaaki Yasunaga, Teruo Kato, Nobuo PLoS One Research Article To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal. Public Library of Science 2013-05-21 /pmc/articles/PMC3660326/ /pubmed/23704970 http://dx.doi.org/10.1371/journal.pone.0064017 Text en © 2013 Kaihatsu et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kaihatsu, Kunihiro
Sawada, Shinjiro
Nakamura, Shota
Nakaya, Takaaki
Yasunaga, Teruo
Kato, Nobuo
Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid
title Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid
title_full Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid
title_fullStr Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid
title_full_unstemmed Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid
title_short Sequence-Specific and Visual Identification of the Influenza Virus NS Gene by Azobenzene-Tethered Bis-Peptide Nucleic Acid
title_sort sequence-specific and visual identification of the influenza virus ns gene by azobenzene-tethered bis-peptide nucleic acid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660326/
https://www.ncbi.nlm.nih.gov/pubmed/23704970
http://dx.doi.org/10.1371/journal.pone.0064017
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