Comparison of Internal Ribosome Entry Site (IRES) and Furin-2A (F2A) for Monoclonal Antibody Expression Level and Quality in CHO Cells

Four versions of tricistronic vectors expressing IgG1 light chain (LC), IgG1 heavy chain (HC), and dihydrofolate reductase (DHFR) in one transcript were designed to compare internal ribosome entry site (IRES) and furin-2A (F2A) for their influence on monoclonal antibody (mAb) expression level and qu...

Descripción completa

Detalles Bibliográficos
Autores principales: Ho, Steven C. L., Bardor, Muriel, Li, Bin, Lee, Jia Juan, Song, Zhiwei, Tong, Yen Wah, Goh, Lin-Tang, Yang, Yuansheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660568/
https://www.ncbi.nlm.nih.gov/pubmed/23704898
http://dx.doi.org/10.1371/journal.pone.0063247

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660568/
https://www.ncbi.nlm.nih.gov/pubmed/23704898
http://dx.doi.org/10.1371/journal.pone.0063247

Ejemplares similares