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Fragmentation of Deprotonated Diacylhydrazine Derivatives in Electrospray Ionization Tandem Mass Spectrometry: Generation of Acid Anions via Intramolecular Rearrangement

The gas-phase fragmentation pathways of deprotonated diacylhydrazine derivatives (R(1)(C = O)-N(t-Bu)NH(C = O)R(2), Compounds 1–6) were investigated by the combination of electrospray ionization tandem mass spectrometry (ESI-MS/MS) and theoretical calculations. Upon collisional activation, the depro...

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Autores principales: Jiang, Kezhi, Zhang, Hu, Wang, Jianmei, Li, Fei, Qian, Mingrong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660572/
https://www.ncbi.nlm.nih.gov/pubmed/23704891
http://dx.doi.org/10.1371/journal.pone.0063097
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author Jiang, Kezhi
Zhang, Hu
Wang, Jianmei
Li, Fei
Qian, Mingrong
author_facet Jiang, Kezhi
Zhang, Hu
Wang, Jianmei
Li, Fei
Qian, Mingrong
author_sort Jiang, Kezhi
collection PubMed
description The gas-phase fragmentation pathways of deprotonated diacylhydrazine derivatives (R(1)(C = O)-N(t-Bu)NH(C = O)R(2), Compounds 1–6) were investigated by the combination of electrospray ionization tandem mass spectrometry (ESI-MS/MS) and theoretical calculations. Upon collisional activation, the deprotonated molecular ions [M – H](−) dissociate in two reaction channels, both of which involve intramolecular rearrangement. The main product ion is confirmed to be an anionic acid species, [R(1)-CO(2)](−), generated through intramolecular rearrangement of [M – H](−) initiated by the nucleophilic attack of the amide O6 on the carbonyl C2 (Path-1). The minor fragment channel (Path-2) involves methylpropene elimination of the precursor ion, followed by a similar nucleophilic displacement reaction to produce another acid anion [R(2)-CO(2)](−). Density functional theory calculations at the B3LYP/6-31+G(d,p) level indicate that Path-1 is more favorable than Path-2 for dissociation of the deprotonated halofenozide.
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spelling pubmed-36605722013-05-23 Fragmentation of Deprotonated Diacylhydrazine Derivatives in Electrospray Ionization Tandem Mass Spectrometry: Generation of Acid Anions via Intramolecular Rearrangement Jiang, Kezhi Zhang, Hu Wang, Jianmei Li, Fei Qian, Mingrong PLoS One Research Article The gas-phase fragmentation pathways of deprotonated diacylhydrazine derivatives (R(1)(C = O)-N(t-Bu)NH(C = O)R(2), Compounds 1–6) were investigated by the combination of electrospray ionization tandem mass spectrometry (ESI-MS/MS) and theoretical calculations. Upon collisional activation, the deprotonated molecular ions [M – H](−) dissociate in two reaction channels, both of which involve intramolecular rearrangement. The main product ion is confirmed to be an anionic acid species, [R(1)-CO(2)](−), generated through intramolecular rearrangement of [M – H](−) initiated by the nucleophilic attack of the amide O6 on the carbonyl C2 (Path-1). The minor fragment channel (Path-2) involves methylpropene elimination of the precursor ion, followed by a similar nucleophilic displacement reaction to produce another acid anion [R(2)-CO(2)](−). Density functional theory calculations at the B3LYP/6-31+G(d,p) level indicate that Path-1 is more favorable than Path-2 for dissociation of the deprotonated halofenozide. Public Library of Science 2013-05-21 /pmc/articles/PMC3660572/ /pubmed/23704891 http://dx.doi.org/10.1371/journal.pone.0063097 Text en © 2013 Jiang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Jiang, Kezhi
Zhang, Hu
Wang, Jianmei
Li, Fei
Qian, Mingrong
Fragmentation of Deprotonated Diacylhydrazine Derivatives in Electrospray Ionization Tandem Mass Spectrometry: Generation of Acid Anions via Intramolecular Rearrangement
title Fragmentation of Deprotonated Diacylhydrazine Derivatives in Electrospray Ionization Tandem Mass Spectrometry: Generation of Acid Anions via Intramolecular Rearrangement
title_full Fragmentation of Deprotonated Diacylhydrazine Derivatives in Electrospray Ionization Tandem Mass Spectrometry: Generation of Acid Anions via Intramolecular Rearrangement
title_fullStr Fragmentation of Deprotonated Diacylhydrazine Derivatives in Electrospray Ionization Tandem Mass Spectrometry: Generation of Acid Anions via Intramolecular Rearrangement
title_full_unstemmed Fragmentation of Deprotonated Diacylhydrazine Derivatives in Electrospray Ionization Tandem Mass Spectrometry: Generation of Acid Anions via Intramolecular Rearrangement
title_short Fragmentation of Deprotonated Diacylhydrazine Derivatives in Electrospray Ionization Tandem Mass Spectrometry: Generation of Acid Anions via Intramolecular Rearrangement
title_sort fragmentation of deprotonated diacylhydrazine derivatives in electrospray ionization tandem mass spectrometry: generation of acid anions via intramolecular rearrangement
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660572/
https://www.ncbi.nlm.nih.gov/pubmed/23704891
http://dx.doi.org/10.1371/journal.pone.0063097
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