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Immunomodulatory activity of methanolic extracts of fruits and bark of Ficus glomerata Roxb. in mice and on human neutrophils

OBJECTIVE: To evaluate the immunomodulatory activity of methanolic extracts of fruit and bark of Ficus glomerata Roxb. on cyclophosphamide-induced myelosuppression in mice and the phagocytic effect on human neutrophils. MATERIALS AND METHODS: Methanolic extracts of fruits and bark of Ficus glomerata...

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Autores principales: Heroor, Sanjeev, Beknal, Arun Kumar, Mahurkar, Nitin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660923/
https://www.ncbi.nlm.nih.gov/pubmed/23716887
http://dx.doi.org/10.4103/0253-7613.108287
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author Heroor, Sanjeev
Beknal, Arun Kumar
Mahurkar, Nitin
author_facet Heroor, Sanjeev
Beknal, Arun Kumar
Mahurkar, Nitin
author_sort Heroor, Sanjeev
collection PubMed
description OBJECTIVE: To evaluate the immunomodulatory activity of methanolic extracts of fruit and bark of Ficus glomerata Roxb. on cyclophosphamide-induced myelosuppression in mice and the phagocytic effect on human neutrophils. MATERIALS AND METHODS: Methanolic extracts of fruits and bark of Ficus glomerata Roxb. at two dose levels of 250and 500 mg/kg p.o. were administered for 13 days to albino mice and cyclophosphamide (30 mg/kg i.p.) was administered on 11(th),12(th), and 13(th) days, 1 hour after the administration of the respective treatment. On 14(th) day blood was collected and the hematological parameters were evaluated. The two extracts in the concentration range 100,50,25,12 and 6.25 μg were also tested for phagocytic effect on human neutrophils using the in vitro models–nitroblue tetrazolium (NBT) dye test, phagocytosis of Candida albicans, and chemotaxis assay. RESULTS: Methanolic extracts of fruit and bark of Ficus glomerata Roxb. showed significant counteracting effect (P < 0.01) to cyclophosphamide-induced reduction in total WBC, differential leucocyte count, platelet counts, RBC counts, and hemoglobin levels. The extracts of the plant in the concentration range 100,50,25,12, and 6.25 μg also showed significant (P < 0.01) phagocytic effect on human neutrophils in the parameters studied. CONCLUSION: Methanolic extracts of fruits and bark of Ficus glomerata Roxb. exhibited immunomodulatory property in both in vivo and in vitro models.
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spelling pubmed-36609232013-05-28 Immunomodulatory activity of methanolic extracts of fruits and bark of Ficus glomerata Roxb. in mice and on human neutrophils Heroor, Sanjeev Beknal, Arun Kumar Mahurkar, Nitin Indian J Pharmacol Research Article OBJECTIVE: To evaluate the immunomodulatory activity of methanolic extracts of fruit and bark of Ficus glomerata Roxb. on cyclophosphamide-induced myelosuppression in mice and the phagocytic effect on human neutrophils. MATERIALS AND METHODS: Methanolic extracts of fruits and bark of Ficus glomerata Roxb. at two dose levels of 250and 500 mg/kg p.o. were administered for 13 days to albino mice and cyclophosphamide (30 mg/kg i.p.) was administered on 11(th),12(th), and 13(th) days, 1 hour after the administration of the respective treatment. On 14(th) day blood was collected and the hematological parameters were evaluated. The two extracts in the concentration range 100,50,25,12 and 6.25 μg were also tested for phagocytic effect on human neutrophils using the in vitro models–nitroblue tetrazolium (NBT) dye test, phagocytosis of Candida albicans, and chemotaxis assay. RESULTS: Methanolic extracts of fruit and bark of Ficus glomerata Roxb. showed significant counteracting effect (P < 0.01) to cyclophosphamide-induced reduction in total WBC, differential leucocyte count, platelet counts, RBC counts, and hemoglobin levels. The extracts of the plant in the concentration range 100,50,25,12, and 6.25 μg also showed significant (P < 0.01) phagocytic effect on human neutrophils in the parameters studied. CONCLUSION: Methanolic extracts of fruits and bark of Ficus glomerata Roxb. exhibited immunomodulatory property in both in vivo and in vitro models. Medknow Publications & Media Pvt Ltd 2013 /pmc/articles/PMC3660923/ /pubmed/23716887 http://dx.doi.org/10.4103/0253-7613.108287 Text en Copyright: © Indian Journal of Pharmacology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Heroor, Sanjeev
Beknal, Arun Kumar
Mahurkar, Nitin
Immunomodulatory activity of methanolic extracts of fruits and bark of Ficus glomerata Roxb. in mice and on human neutrophils
title Immunomodulatory activity of methanolic extracts of fruits and bark of Ficus glomerata Roxb. in mice and on human neutrophils
title_full Immunomodulatory activity of methanolic extracts of fruits and bark of Ficus glomerata Roxb. in mice and on human neutrophils
title_fullStr Immunomodulatory activity of methanolic extracts of fruits and bark of Ficus glomerata Roxb. in mice and on human neutrophils
title_full_unstemmed Immunomodulatory activity of methanolic extracts of fruits and bark of Ficus glomerata Roxb. in mice and on human neutrophils
title_short Immunomodulatory activity of methanolic extracts of fruits and bark of Ficus glomerata Roxb. in mice and on human neutrophils
title_sort immunomodulatory activity of methanolic extracts of fruits and bark of ficus glomerata roxb. in mice and on human neutrophils
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3660923/
https://www.ncbi.nlm.nih.gov/pubmed/23716887
http://dx.doi.org/10.4103/0253-7613.108287
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