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Ordering the bestiary of genetic elements transmissible by conjugation

Phylogenetic reconstruction of three highly conserved proteins involved in bacterial conjugation (relaxase, coupling protein and a type IV secretion system ATPase) allowed the classification of transmissible elements in relaxase MOB families and mating pair formation MPF groups. These evolutionary s...

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Detalles Bibliográficos
Autores principales: Garcillán-Barcia, Maria Pilar, de la Cruz, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Landes Bioscience 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3661145/
https://www.ncbi.nlm.nih.gov/pubmed/23734300
http://dx.doi.org/10.4161/mge.24263
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author Garcillán-Barcia, Maria Pilar
de la Cruz, Fernando
author_facet Garcillán-Barcia, Maria Pilar
de la Cruz, Fernando
author_sort Garcillán-Barcia, Maria Pilar
collection PubMed
description Phylogenetic reconstruction of three highly conserved proteins involved in bacterial conjugation (relaxase, coupling protein and a type IV secretion system ATPase) allowed the classification of transmissible elements in relaxase MOB families and mating pair formation MPF groups. These evolutionary studies point to the existence of a limited number of module combinations in transmissible elements, preferentially associated with specific genetic or environmental backgrounds. A practical protocol based on the MOB classification was implemented to detect and assort transmissible plasmids and integrative elements from γ-Proteobacteria. It was called “Degenerate Primer MOB Typing” or DPMT. It resulted in a powerful technique that discovers not only backbones related to previously classified elements (typically by PCR-based replicon typing or PBRT), but also distant new members sharing a common evolutionary ancestor. The DPMT method, conjointly with PBRT, promises to be useful to gain information on plasmid backbones and helpful to investigate the dissemination routes of transmissible elements in microbial ecosystems.
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spelling pubmed-36611452013-06-03 Ordering the bestiary of genetic elements transmissible by conjugation Garcillán-Barcia, Maria Pilar de la Cruz, Fernando Mob Genet Elements Commentary Phylogenetic reconstruction of three highly conserved proteins involved in bacterial conjugation (relaxase, coupling protein and a type IV secretion system ATPase) allowed the classification of transmissible elements in relaxase MOB families and mating pair formation MPF groups. These evolutionary studies point to the existence of a limited number of module combinations in transmissible elements, preferentially associated with specific genetic or environmental backgrounds. A practical protocol based on the MOB classification was implemented to detect and assort transmissible plasmids and integrative elements from γ-Proteobacteria. It was called “Degenerate Primer MOB Typing” or DPMT. It resulted in a powerful technique that discovers not only backbones related to previously classified elements (typically by PCR-based replicon typing or PBRT), but also distant new members sharing a common evolutionary ancestor. The DPMT method, conjointly with PBRT, promises to be useful to gain information on plasmid backbones and helpful to investigate the dissemination routes of transmissible elements in microbial ecosystems. Landes Bioscience 2013-01-01 2013-01-01 /pmc/articles/PMC3661145/ /pubmed/23734300 http://dx.doi.org/10.4161/mge.24263 Text en Copyright © 2013 Landes Bioscience http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Commentary
Garcillán-Barcia, Maria Pilar
de la Cruz, Fernando
Ordering the bestiary of genetic elements transmissible by conjugation
title Ordering the bestiary of genetic elements transmissible by conjugation
title_full Ordering the bestiary of genetic elements transmissible by conjugation
title_fullStr Ordering the bestiary of genetic elements transmissible by conjugation
title_full_unstemmed Ordering the bestiary of genetic elements transmissible by conjugation
title_short Ordering the bestiary of genetic elements transmissible by conjugation
title_sort ordering the bestiary of genetic elements transmissible by conjugation
topic Commentary
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3661145/
https://www.ncbi.nlm.nih.gov/pubmed/23734300
http://dx.doi.org/10.4161/mge.24263
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