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HDAC mediated suppression of histone turnover promotes epigenetic stability of heterochromatin

Heterochromatin causes epigenetic repression that can be transmitted through multiple cell divisions. However, the mechanisms underlying silencing and stability of heterochromatin are not fully understood. We show that heterochromatin differs from euchromatin in histone turnover, and identify histon...

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Detalles Bibliográficos
Autores principales: Aygün, Ozan, Mehta, Sameet, Grewal, Shiv I. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3661211/
https://www.ncbi.nlm.nih.gov/pubmed/23604080
http://dx.doi.org/10.1038/nsmb.2565
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author Aygün, Ozan
Mehta, Sameet
Grewal, Shiv I. S.
author_facet Aygün, Ozan
Mehta, Sameet
Grewal, Shiv I. S.
author_sort Aygün, Ozan
collection PubMed
description Heterochromatin causes epigenetic repression that can be transmitted through multiple cell divisions. However, the mechanisms underlying silencing and stability of heterochromatin are not fully understood. We show that heterochromatin differs from euchromatin in histone turnover, and identify histone deacetylase (HDAC) Clr3 as a factor required for inhibiting histone turnover across heterochromatin domains in Schizosaccharomyces pombe. Loss of RNAi factors, Clr4 methyltransferase, or HP1 proteins involved in HDAC localization causes increased histone turnover across pericentromeric domains. Clr3 also affects histone turnover at the silent mating–type region where it can be recruited by alternative mechanisms acting in parallel to H3K9me–HP1. Importantly, the JmjC–domain protein Epe1 promotes histone exchange, and loss of Epe1 suppresses both histone turnover and defects in heterochromatic silencing. Our results suggest that heterochromatic silencing factors preclude histone turnover to promote silencing and inheritance of repressive chromatin.
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spelling pubmed-36612112013-11-01 HDAC mediated suppression of histone turnover promotes epigenetic stability of heterochromatin Aygün, Ozan Mehta, Sameet Grewal, Shiv I. S. Nat Struct Mol Biol Article Heterochromatin causes epigenetic repression that can be transmitted through multiple cell divisions. However, the mechanisms underlying silencing and stability of heterochromatin are not fully understood. We show that heterochromatin differs from euchromatin in histone turnover, and identify histone deacetylase (HDAC) Clr3 as a factor required for inhibiting histone turnover across heterochromatin domains in Schizosaccharomyces pombe. Loss of RNAi factors, Clr4 methyltransferase, or HP1 proteins involved in HDAC localization causes increased histone turnover across pericentromeric domains. Clr3 also affects histone turnover at the silent mating–type region where it can be recruited by alternative mechanisms acting in parallel to H3K9me–HP1. Importantly, the JmjC–domain protein Epe1 promotes histone exchange, and loss of Epe1 suppresses both histone turnover and defects in heterochromatic silencing. Our results suggest that heterochromatic silencing factors preclude histone turnover to promote silencing and inheritance of repressive chromatin. 2013-04-21 2013-05 /pmc/articles/PMC3661211/ /pubmed/23604080 http://dx.doi.org/10.1038/nsmb.2565 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Aygün, Ozan
Mehta, Sameet
Grewal, Shiv I. S.
HDAC mediated suppression of histone turnover promotes epigenetic stability of heterochromatin
title HDAC mediated suppression of histone turnover promotes epigenetic stability of heterochromatin
title_full HDAC mediated suppression of histone turnover promotes epigenetic stability of heterochromatin
title_fullStr HDAC mediated suppression of histone turnover promotes epigenetic stability of heterochromatin
title_full_unstemmed HDAC mediated suppression of histone turnover promotes epigenetic stability of heterochromatin
title_short HDAC mediated suppression of histone turnover promotes epigenetic stability of heterochromatin
title_sort hdac mediated suppression of histone turnover promotes epigenetic stability of heterochromatin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3661211/
https://www.ncbi.nlm.nih.gov/pubmed/23604080
http://dx.doi.org/10.1038/nsmb.2565
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