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Interferon-γ Triggers Hepatic Stellate Cell-Mediated Immune Regulation through MEK/ERK Signaling Pathway
Hepatic stellate cells (HSCs) interact with immune cells to actively participate in regulating immune response in the liver which is mediated by the effector molecules, including B7-H1. We demonstrated here that expression of B7-H1 on HSCs was markedly enhanced by interferon-(IFN-) γ stimulation. IF...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662191/ https://www.ncbi.nlm.nih.gov/pubmed/23737812 http://dx.doi.org/10.1155/2013/389807 |
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author | Gu, Xiaodong Wang, Yan Xiang, Jianbin Chen, Zongyou Wang, Lianfu Lu, Lina Qian, Shiguang |
author_facet | Gu, Xiaodong Wang, Yan Xiang, Jianbin Chen, Zongyou Wang, Lianfu Lu, Lina Qian, Shiguang |
author_sort | Gu, Xiaodong |
collection | PubMed |
description | Hepatic stellate cells (HSCs) interact with immune cells to actively participate in regulating immune response in the liver which is mediated by the effector molecules, including B7-H1. We demonstrated here that expression of B7-H1 on HSCs was markedly enhanced by interferon-(IFN-) γ stimulation. IFN-γ stimulated HSCs inhibited T-cell proliferation via induction of T-cell apoptosis (22.1% ± 1.6%). This immunosuppressive effect was inhibited by preincubation with an anti-B7-H1 antibody, or inhibitor of the MEK/ERK pathway inhibited IFN-γ mediated expression of B7-H1. Thus, regulation of B7-H1 expression on HSCs by IFN-γ represents an important mechanism that regulates immune responses in the liver favoring tolerogenicity rather than immunogenicity. Involvement of MEK/ERK pathway provides a novel target for therapeutic approaches. |
format | Online Article Text |
id | pubmed-3662191 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36621912013-06-04 Interferon-γ Triggers Hepatic Stellate Cell-Mediated Immune Regulation through MEK/ERK Signaling Pathway Gu, Xiaodong Wang, Yan Xiang, Jianbin Chen, Zongyou Wang, Lianfu Lu, Lina Qian, Shiguang Clin Dev Immunol Research Article Hepatic stellate cells (HSCs) interact with immune cells to actively participate in regulating immune response in the liver which is mediated by the effector molecules, including B7-H1. We demonstrated here that expression of B7-H1 on HSCs was markedly enhanced by interferon-(IFN-) γ stimulation. IFN-γ stimulated HSCs inhibited T-cell proliferation via induction of T-cell apoptosis (22.1% ± 1.6%). This immunosuppressive effect was inhibited by preincubation with an anti-B7-H1 antibody, or inhibitor of the MEK/ERK pathway inhibited IFN-γ mediated expression of B7-H1. Thus, regulation of B7-H1 expression on HSCs by IFN-γ represents an important mechanism that regulates immune responses in the liver favoring tolerogenicity rather than immunogenicity. Involvement of MEK/ERK pathway provides a novel target for therapeutic approaches. Hindawi Publishing Corporation 2013 2013-05-07 /pmc/articles/PMC3662191/ /pubmed/23737812 http://dx.doi.org/10.1155/2013/389807 Text en Copyright © 2013 Xiaodong Gu et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gu, Xiaodong Wang, Yan Xiang, Jianbin Chen, Zongyou Wang, Lianfu Lu, Lina Qian, Shiguang Interferon-γ Triggers Hepatic Stellate Cell-Mediated Immune Regulation through MEK/ERK Signaling Pathway |
title | Interferon-γ Triggers Hepatic Stellate Cell-Mediated Immune Regulation through MEK/ERK Signaling Pathway |
title_full | Interferon-γ Triggers Hepatic Stellate Cell-Mediated Immune Regulation through MEK/ERK Signaling Pathway |
title_fullStr | Interferon-γ Triggers Hepatic Stellate Cell-Mediated Immune Regulation through MEK/ERK Signaling Pathway |
title_full_unstemmed | Interferon-γ Triggers Hepatic Stellate Cell-Mediated Immune Regulation through MEK/ERK Signaling Pathway |
title_short | Interferon-γ Triggers Hepatic Stellate Cell-Mediated Immune Regulation through MEK/ERK Signaling Pathway |
title_sort | interferon-γ triggers hepatic stellate cell-mediated immune regulation through mek/erk signaling pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662191/ https://www.ncbi.nlm.nih.gov/pubmed/23737812 http://dx.doi.org/10.1155/2013/389807 |
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