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Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia

BACKGROUND: The purpose of this study is to image the effect of diabetes on expression of receptor for advanced glycation endproducts (RAGE) in limb ischemia in live animals. METHODS: Male wild-type C57BL/6 mice were either made diabetic or left as control. Two months later, diabetic and non-diabeti...

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Autores principales: Tekabe, Yared, Kollaros, Maria, Li, Chong, Zhang, Geping, Schmidt, Ann Marie, Johnson, Lynne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662588/
https://www.ncbi.nlm.nih.gov/pubmed/23663412
http://dx.doi.org/10.1186/2191-219X-3-37
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author Tekabe, Yared
Kollaros, Maria
Li, Chong
Zhang, Geping
Schmidt, Ann Marie
Johnson, Lynne
author_facet Tekabe, Yared
Kollaros, Maria
Li, Chong
Zhang, Geping
Schmidt, Ann Marie
Johnson, Lynne
author_sort Tekabe, Yared
collection PubMed
description BACKGROUND: The purpose of this study is to image the effect of diabetes on expression of receptor for advanced glycation endproducts (RAGE) in limb ischemia in live animals. METHODS: Male wild-type C57BL/6 mice were either made diabetic or left as control. Two months later, diabetic and non-diabetic mice underwent left femoral artery ligation. The right leg served as lesion control. Five days later, mice were injected with 15.1 ± 4.4 MBq (99m)Tc-anti-RAGE F(ab’)(2) and 4 to 5 h later (blood pool clearance) underwent SPECT/CT imaging. At the completion of imaging, mice were euthanized, hind limbs counted and sectioned, and scans reconstructed. Regions of interest were drawn on serial transverse sections comprising the hind limbs and activity in millicuries summed and divided by the injected dose (ID). Quantitative histology was performed for RAGE staining and angiogenesis. RESULTS: Uptake of (99m)Tc-anti-RAGE F(ab')(2) as %ID × 10(−3) was higher in the left (ischemic) limbs for the diabetic mice (n = 8) compared to non-diabetic mice (n = 8) (1.20 ± 0.44% vs. 0.49 ± 0.40%; P = 0.0007) and corresponded to less angiogenesis in the diabetic mice. Uptake was also higher in the right limbs of diabetic compared to non-diabetic animals (0.82 ± 0.33% vs. 0.40 ± 0.14%; P = 0.0004). CONCLUSIONS: These data show the feasibility of imaging and quantifying the effect of diabetes on RAGE expression in limb ischemia.
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spelling pubmed-36625882013-05-24 Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia Tekabe, Yared Kollaros, Maria Li, Chong Zhang, Geping Schmidt, Ann Marie Johnson, Lynne EJNMMI Res Original Research BACKGROUND: The purpose of this study is to image the effect of diabetes on expression of receptor for advanced glycation endproducts (RAGE) in limb ischemia in live animals. METHODS: Male wild-type C57BL/6 mice were either made diabetic or left as control. Two months later, diabetic and non-diabetic mice underwent left femoral artery ligation. The right leg served as lesion control. Five days later, mice were injected with 15.1 ± 4.4 MBq (99m)Tc-anti-RAGE F(ab’)(2) and 4 to 5 h later (blood pool clearance) underwent SPECT/CT imaging. At the completion of imaging, mice were euthanized, hind limbs counted and sectioned, and scans reconstructed. Regions of interest were drawn on serial transverse sections comprising the hind limbs and activity in millicuries summed and divided by the injected dose (ID). Quantitative histology was performed for RAGE staining and angiogenesis. RESULTS: Uptake of (99m)Tc-anti-RAGE F(ab')(2) as %ID × 10(−3) was higher in the left (ischemic) limbs for the diabetic mice (n = 8) compared to non-diabetic mice (n = 8) (1.20 ± 0.44% vs. 0.49 ± 0.40%; P = 0.0007) and corresponded to less angiogenesis in the diabetic mice. Uptake was also higher in the right limbs of diabetic compared to non-diabetic animals (0.82 ± 0.33% vs. 0.40 ± 0.14%; P = 0.0004). CONCLUSIONS: These data show the feasibility of imaging and quantifying the effect of diabetes on RAGE expression in limb ischemia. Springer 2013-05-11 /pmc/articles/PMC3662588/ /pubmed/23663412 http://dx.doi.org/10.1186/2191-219X-3-37 Text en Copyright ©2013 Tekabe et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Tekabe, Yared
Kollaros, Maria
Li, Chong
Zhang, Geping
Schmidt, Ann Marie
Johnson, Lynne
Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia
title Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia
title_full Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia
title_fullStr Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia
title_full_unstemmed Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia
title_short Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia
title_sort imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662588/
https://www.ncbi.nlm.nih.gov/pubmed/23663412
http://dx.doi.org/10.1186/2191-219X-3-37
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