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Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia
BACKGROUND: The purpose of this study is to image the effect of diabetes on expression of receptor for advanced glycation endproducts (RAGE) in limb ischemia in live animals. METHODS: Male wild-type C57BL/6 mice were either made diabetic or left as control. Two months later, diabetic and non-diabeti...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662588/ https://www.ncbi.nlm.nih.gov/pubmed/23663412 http://dx.doi.org/10.1186/2191-219X-3-37 |
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author | Tekabe, Yared Kollaros, Maria Li, Chong Zhang, Geping Schmidt, Ann Marie Johnson, Lynne |
author_facet | Tekabe, Yared Kollaros, Maria Li, Chong Zhang, Geping Schmidt, Ann Marie Johnson, Lynne |
author_sort | Tekabe, Yared |
collection | PubMed |
description | BACKGROUND: The purpose of this study is to image the effect of diabetes on expression of receptor for advanced glycation endproducts (RAGE) in limb ischemia in live animals. METHODS: Male wild-type C57BL/6 mice were either made diabetic or left as control. Two months later, diabetic and non-diabetic mice underwent left femoral artery ligation. The right leg served as lesion control. Five days later, mice were injected with 15.1 ± 4.4 MBq (99m)Tc-anti-RAGE F(ab’)(2) and 4 to 5 h later (blood pool clearance) underwent SPECT/CT imaging. At the completion of imaging, mice were euthanized, hind limbs counted and sectioned, and scans reconstructed. Regions of interest were drawn on serial transverse sections comprising the hind limbs and activity in millicuries summed and divided by the injected dose (ID). Quantitative histology was performed for RAGE staining and angiogenesis. RESULTS: Uptake of (99m)Tc-anti-RAGE F(ab')(2) as %ID × 10(−3) was higher in the left (ischemic) limbs for the diabetic mice (n = 8) compared to non-diabetic mice (n = 8) (1.20 ± 0.44% vs. 0.49 ± 0.40%; P = 0.0007) and corresponded to less angiogenesis in the diabetic mice. Uptake was also higher in the right limbs of diabetic compared to non-diabetic animals (0.82 ± 0.33% vs. 0.40 ± 0.14%; P = 0.0004). CONCLUSIONS: These data show the feasibility of imaging and quantifying the effect of diabetes on RAGE expression in limb ischemia. |
format | Online Article Text |
id | pubmed-3662588 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Springer |
record_format | MEDLINE/PubMed |
spelling | pubmed-36625882013-05-24 Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia Tekabe, Yared Kollaros, Maria Li, Chong Zhang, Geping Schmidt, Ann Marie Johnson, Lynne EJNMMI Res Original Research BACKGROUND: The purpose of this study is to image the effect of diabetes on expression of receptor for advanced glycation endproducts (RAGE) in limb ischemia in live animals. METHODS: Male wild-type C57BL/6 mice were either made diabetic or left as control. Two months later, diabetic and non-diabetic mice underwent left femoral artery ligation. The right leg served as lesion control. Five days later, mice were injected with 15.1 ± 4.4 MBq (99m)Tc-anti-RAGE F(ab’)(2) and 4 to 5 h later (blood pool clearance) underwent SPECT/CT imaging. At the completion of imaging, mice were euthanized, hind limbs counted and sectioned, and scans reconstructed. Regions of interest were drawn on serial transverse sections comprising the hind limbs and activity in millicuries summed and divided by the injected dose (ID). Quantitative histology was performed for RAGE staining and angiogenesis. RESULTS: Uptake of (99m)Tc-anti-RAGE F(ab')(2) as %ID × 10(−3) was higher in the left (ischemic) limbs for the diabetic mice (n = 8) compared to non-diabetic mice (n = 8) (1.20 ± 0.44% vs. 0.49 ± 0.40%; P = 0.0007) and corresponded to less angiogenesis in the diabetic mice. Uptake was also higher in the right limbs of diabetic compared to non-diabetic animals (0.82 ± 0.33% vs. 0.40 ± 0.14%; P = 0.0004). CONCLUSIONS: These data show the feasibility of imaging and quantifying the effect of diabetes on RAGE expression in limb ischemia. Springer 2013-05-11 /pmc/articles/PMC3662588/ /pubmed/23663412 http://dx.doi.org/10.1186/2191-219X-3-37 Text en Copyright ©2013 Tekabe et al.; licensee Springer. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Research Tekabe, Yared Kollaros, Maria Li, Chong Zhang, Geping Schmidt, Ann Marie Johnson, Lynne Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia |
title | Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia |
title_full | Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia |
title_fullStr | Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia |
title_full_unstemmed | Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia |
title_short | Imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia |
title_sort | imaging receptor for advanced glycation end product expression in mouse model of hind limb ischemia |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662588/ https://www.ncbi.nlm.nih.gov/pubmed/23663412 http://dx.doi.org/10.1186/2191-219X-3-37 |
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