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A standard vector for the chromosomal integration and characterization of BioBrick™ parts in Escherichia coli
BACKGROUND: The chromosomal integration of biological parts in the host genome enables the engineering of plasmid-free stable strains with single-copy insertions of the desired gene networks. Although different integrative vectors were proposed, no standard pre-assembled genetic tool is available to...
Autores principales: | Zucca, Susanna, Pasotti, Lorenzo, Politi, Nicolò, Cusella De Angelis, Maria Gabriella, Magni, Paolo |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3662617/ https://www.ncbi.nlm.nih.gov/pubmed/23663425 http://dx.doi.org/10.1186/1754-1611-7-12 |
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