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Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes

The electrocatalytical property of single-wall carbon nanotube (SWNT) modified electrode toward NADH detection was explored by cyclic voltammetry and amperometry techniques. The experimental results show that SWNT decrease the overvoltage required for oxidation of NADH (to +300 mV vs. Ag/AgCl) and t...

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Autores principales: Arvinte, Adina, Rotariu, Lucian, Bala, Camelia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2008
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3663007/
https://www.ncbi.nlm.nih.gov/pubmed/27879776
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author Arvinte, Adina
Rotariu, Lucian
Bala, Camelia
author_facet Arvinte, Adina
Rotariu, Lucian
Bala, Camelia
author_sort Arvinte, Adina
collection PubMed
description The electrocatalytical property of single-wall carbon nanotube (SWNT) modified electrode toward NADH detection was explored by cyclic voltammetry and amperometry techniques. The experimental results show that SWNT decrease the overvoltage required for oxidation of NADH (to +300 mV vs. Ag/AgCl) and this property make them suitable for dehydrogenases based biosensors. The behavior of the SWNT modified biosensor for L-malic acid was studied as an example for dehydrogenases biosensor. The amperometric measurements indicate that malate dehydrogenase (MDH) can be strongly adsorbed on the surface of the SWNT-modified electrode to form an approximate monolayer film. Enzyme immobilization in Nafion membrane can increase the biosensor stability. A linear calibration curve was obtained for L-malic acid concentrations between 0.2 and 1mM.
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spelling pubmed-36630072013-05-30 Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes Arvinte, Adina Rotariu, Lucian Bala, Camelia Sensors (Basel) Full Research Paper The electrocatalytical property of single-wall carbon nanotube (SWNT) modified electrode toward NADH detection was explored by cyclic voltammetry and amperometry techniques. The experimental results show that SWNT decrease the overvoltage required for oxidation of NADH (to +300 mV vs. Ag/AgCl) and this property make them suitable for dehydrogenases based biosensors. The behavior of the SWNT modified biosensor for L-malic acid was studied as an example for dehydrogenases biosensor. The amperometric measurements indicate that malate dehydrogenase (MDH) can be strongly adsorbed on the surface of the SWNT-modified electrode to form an approximate monolayer film. Enzyme immobilization in Nafion membrane can increase the biosensor stability. A linear calibration curve was obtained for L-malic acid concentrations between 0.2 and 1mM. Molecular Diversity Preservation International (MDPI) 2008-03-03 /pmc/articles/PMC3663007/ /pubmed/27879776 Text en © 2008 by MDPI Reproduction is permitted for noncommercial purposes.
spellingShingle Full Research Paper
Arvinte, Adina
Rotariu, Lucian
Bala, Camelia
Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes
title Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes
title_full Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes
title_fullStr Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes
title_full_unstemmed Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes
title_short Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes
title_sort amperometric low-potential detection of malic acid using single-wall carbon nanotubes based electrodes
topic Full Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3663007/
https://www.ncbi.nlm.nih.gov/pubmed/27879776
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