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Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes
The electrocatalytical property of single-wall carbon nanotube (SWNT) modified electrode toward NADH detection was explored by cyclic voltammetry and amperometry techniques. The experimental results show that SWNT decrease the overvoltage required for oxidation of NADH (to +300 mV vs. Ag/AgCl) and t...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Molecular Diversity Preservation International (MDPI)
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3663007/ https://www.ncbi.nlm.nih.gov/pubmed/27879776 |
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author | Arvinte, Adina Rotariu, Lucian Bala, Camelia |
author_facet | Arvinte, Adina Rotariu, Lucian Bala, Camelia |
author_sort | Arvinte, Adina |
collection | PubMed |
description | The electrocatalytical property of single-wall carbon nanotube (SWNT) modified electrode toward NADH detection was explored by cyclic voltammetry and amperometry techniques. The experimental results show that SWNT decrease the overvoltage required for oxidation of NADH (to +300 mV vs. Ag/AgCl) and this property make them suitable for dehydrogenases based biosensors. The behavior of the SWNT modified biosensor for L-malic acid was studied as an example for dehydrogenases biosensor. The amperometric measurements indicate that malate dehydrogenase (MDH) can be strongly adsorbed on the surface of the SWNT-modified electrode to form an approximate monolayer film. Enzyme immobilization in Nafion membrane can increase the biosensor stability. A linear calibration curve was obtained for L-malic acid concentrations between 0.2 and 1mM. |
format | Online Article Text |
id | pubmed-3663007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Molecular Diversity Preservation International (MDPI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-36630072013-05-30 Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes Arvinte, Adina Rotariu, Lucian Bala, Camelia Sensors (Basel) Full Research Paper The electrocatalytical property of single-wall carbon nanotube (SWNT) modified electrode toward NADH detection was explored by cyclic voltammetry and amperometry techniques. The experimental results show that SWNT decrease the overvoltage required for oxidation of NADH (to +300 mV vs. Ag/AgCl) and this property make them suitable for dehydrogenases based biosensors. The behavior of the SWNT modified biosensor for L-malic acid was studied as an example for dehydrogenases biosensor. The amperometric measurements indicate that malate dehydrogenase (MDH) can be strongly adsorbed on the surface of the SWNT-modified electrode to form an approximate monolayer film. Enzyme immobilization in Nafion membrane can increase the biosensor stability. A linear calibration curve was obtained for L-malic acid concentrations between 0.2 and 1mM. Molecular Diversity Preservation International (MDPI) 2008-03-03 /pmc/articles/PMC3663007/ /pubmed/27879776 Text en © 2008 by MDPI Reproduction is permitted for noncommercial purposes. |
spellingShingle | Full Research Paper Arvinte, Adina Rotariu, Lucian Bala, Camelia Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes |
title | Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes |
title_full | Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes |
title_fullStr | Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes |
title_full_unstemmed | Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes |
title_short | Amperometric Low-Potential Detection of Malic Acid Using Single-Wall Carbon Nanotubes Based Electrodes |
title_sort | amperometric low-potential detection of malic acid using single-wall carbon nanotubes based electrodes |
topic | Full Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3663007/ https://www.ncbi.nlm.nih.gov/pubmed/27879776 |
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