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In vitro growth of pneumococcal isolates representing 23 different serotypes
BACKGROUND: Although there are established methodologies for pneumococcal carriage studies, recent studies have extended these by conducting a culture amplification step prior to pneumococcal identification or serotyping. However, few data are available comparing the growth of different serotypes. F...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664610/ https://www.ncbi.nlm.nih.gov/pubmed/23701729 http://dx.doi.org/10.1186/1756-0500-6-208 |
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author | Slotved, Hans-Christian Satzke, Catherine |
author_facet | Slotved, Hans-Christian Satzke, Catherine |
author_sort | Slotved, Hans-Christian |
collection | PubMed |
description | BACKGROUND: Although there are established methodologies for pneumococcal carriage studies, recent studies have extended these by conducting a culture amplification step prior to pneumococcal identification or serotyping. However, few data are available comparing the growth of different serotypes. FINDINGS: We compared the growth of individual isolates representing 23 serotypes in serum broth, and Todd-Hewitt broth from four different manufacturers. Following overnight incubation of low inocula, there were differences in the final growth densities of individual isolates. These can be minimised with the use of optimal media. CONCLUSIONS: These data caution against using broth culture amplification of nasopharyngeal samples for some applications. |
format | Online Article Text |
id | pubmed-3664610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-36646102013-05-28 In vitro growth of pneumococcal isolates representing 23 different serotypes Slotved, Hans-Christian Satzke, Catherine BMC Res Notes Short Report BACKGROUND: Although there are established methodologies for pneumococcal carriage studies, recent studies have extended these by conducting a culture amplification step prior to pneumococcal identification or serotyping. However, few data are available comparing the growth of different serotypes. FINDINGS: We compared the growth of individual isolates representing 23 serotypes in serum broth, and Todd-Hewitt broth from four different manufacturers. Following overnight incubation of low inocula, there were differences in the final growth densities of individual isolates. These can be minimised with the use of optimal media. CONCLUSIONS: These data caution against using broth culture amplification of nasopharyngeal samples for some applications. BioMed Central 2013-05-23 /pmc/articles/PMC3664610/ /pubmed/23701729 http://dx.doi.org/10.1186/1756-0500-6-208 Text en Copyright © 2013 Slotved and Satzke; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Report Slotved, Hans-Christian Satzke, Catherine In vitro growth of pneumococcal isolates representing 23 different serotypes |
title | In vitro growth of pneumococcal isolates representing 23 different serotypes |
title_full | In vitro growth of pneumococcal isolates representing 23 different serotypes |
title_fullStr | In vitro growth of pneumococcal isolates representing 23 different serotypes |
title_full_unstemmed | In vitro growth of pneumococcal isolates representing 23 different serotypes |
title_short | In vitro growth of pneumococcal isolates representing 23 different serotypes |
title_sort | in vitro growth of pneumococcal isolates representing 23 different serotypes |
topic | Short Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664610/ https://www.ncbi.nlm.nih.gov/pubmed/23701729 http://dx.doi.org/10.1186/1756-0500-6-208 |
work_keys_str_mv | AT slotvedhanschristian invitrogrowthofpneumococcalisolatesrepresenting23differentserotypes AT satzkecatherine invitrogrowthofpneumococcalisolatesrepresenting23differentserotypes |