Cargando…

Inferring subunit stoichiometry from single molecule photobleaching

Single molecule photobleaching is a powerful tool for determining the stoichiometry of protein complexes. By attaching fluorophores to proteins of interest, the number of associated subunits in a complex can be deduced by imaging single molecules and counting fluorophore photobleaching steps. Becaus...

Descripción completa

Detalles Bibliográficos
Autor principal:	Hines, Keegan E.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Rockefeller University Press 2013
Materias:	Methods and Approaches
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664702/ https://www.ncbi.nlm.nih.gov/pubmed/23712552 http://dx.doi.org/10.1085/jgp.201310988

Ejemplares similares

Determination of parameter identifiability in nonlinear biophysical models: A Bayesian approach
por: Hines, Keegan E., et al.
Publicado: (2014)

A method for imaging single molecules at the plasma membrane of live cells within tissue slices
por: Mashanov, Gregory I., et al.
Publicado: (2020)

Glutathione release through connexin hemichannels: Implications for chemical modification of pores permeable to large molecules
por: Tong, Xuhui, et al.
Publicado: (2015)

A novel voltage-clamp/dye uptake assay reveals saturable transport of molecules through CALHM1 and connexin channels
por: Gaete, Pablo S., et al.
Publicado: (2020)

The method for integrating dual-color fluorescence colocalization and single molecule photobleaching technology on the theophylline sensing platform
por: Zhang, Dong, et al.
Publicado: (2020)

Photobleaching step analysis for robust determination of protein complex stoichiometries
por: Hummert, Johan, et al.
Publicado: (2021)

Transition metal ion FRET to measure short-range distances at the intracellular surface of the plasma membrane
por: Gordon, Sharona E., et al.
Publicado: (2016)

Measuring distances between TRPV1 and the plasma membrane using a noncanonical amino acid and transition metal ion FRET
por: Zagotta, William N., et al.
Publicado: (2016)

Wheat germ agglutinin–conjugated fluorescent pH sensors for visualizing proton fluxes
por: Zhang, Lejie, et al.
Publicado: (2020)

Functional characterization of a ClC transporter by solid-supported membrane electrophysiology
por: Garcia-Celma, Juan, et al.
Publicado: (2013)

Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS
por: Manno, Carlo, et al.
Publicado: (2013)

Molecular dynamics simulations of membrane proteins under asymmetric ionic concentrations
por: Khalili-Araghi, Fatemeh, et al.
Publicado: (2013)

Imaging calcium microdomains within entire astrocyte territories and endfeet with GCaMPs expressed using adeno-associated viruses
por: Shigetomi, Eiji, et al.
Publicado: (2013)

Sarcomere length nanometry in rat neonatal cardiomyocytes expressed with α-actinin–AcGFP in Z discs
por: Shintani, Seine A., et al.
Publicado: (2014)

Imaging P2X4 receptor subcellular distribution, trafficking, and regulation using P2X4-pHluorin
por: Xu, Ji, et al.
Publicado: (2014)

Calmodulation meta-analysis: Predicting calmodulin binding via canonical motif clustering
por: Mruk, Karen, et al.
Publicado: (2014)

Beyond non-integer Hill coefficients: A novel approach to analyzing binding data, applied to Na(+)-driven transporters
por: Ravera, Silvia, et al.
Publicado: (2015)

Zinc flexes its muscle: Correcting a novel analysis of calcium for zinc interference uncovers a method to measure zinc
por: Qian, Cheng, et al.
Publicado: (2016)

bSUM: A bead-supported unilamellar membrane system facilitating unidirectional insertion of membrane proteins into giant vesicles
por: Zheng, Hui, et al.
Publicado: (2016)

New method for determining total calcium content in tissue applied to skeletal muscle with and without calsequestrin
por: Lamboley, Cédric R.H., et al.
Publicado: (2015)

Stabilization of a prokaryotic LAT transporter by random mutagenesis
por: Rodríguez-Banqueri, Arturo, et al.
Publicado: (2016)

Action potentials in Xenopus oocytes triggered by blue light
por: Walther, Florian, et al.
Publicado: (2020)

A high-throughput fluorescence lifetime-based assay to detect binding of myosin-binding protein C to F-actin
por: Bunch, Thomas A., et al.
Publicado: (2021)

A novel method for determining murine skeletal muscle fiber type using autofluorescence lifetimes
por: Manno, Carlo, et al.
Publicado: (2022)

A reconstituted depolarization-induced Ca(2+) release platform for validation of skeletal muscle disease mutations and drug discovery
por: Murayama, Takashi, et al.
Publicado: (2022)

Amyloid-β oligomerization monitored by single-molecule stepwise photobleaching
por: Dresser, Lara, et al.
Publicado: (2021)

Can DyeCycling break the photobleaching limit in single-molecule FRET?
por: Vermeer, Benjamin, et al.
Publicado: (2022)

Path finding methods accounting for stoichiometry in metabolic networks
por: Pey, Jon, et al.
Publicado: (2011)

Tree inference for single-cell data
por: Jahn, Katharina, et al.
Publicado: (2016)

Estimating numbers of intracellular molecules through analysing fluctuations in photobleaching
por: Bakker, Elco, et al.
Publicado: (2019)

Numerical Simulation of the Photobleaching Process in Laser-Induced Fluorescence Photobleaching Anemometer
por: Chen, Yu, et al.
Publicado: (2021)

Determination of Epithelial Na(+) Channel Subunit Stoichiometry from Single-Channel Conductances
por: Anantharam, Arun, et al.
Publicado: (2007)

Analysis of the Subunit Stoichiometries in Viral Entry
por: Magnus, Carsten, et al.
Publicado: (2012)

OncoNEM: inferring tumor evolution from single-cell sequencing data
por: Ross, Edith M., et al.
Publicado: (2016)

Inference of single-cell phylogenies from lineage tracing data using Cassiopeia
por: Jones, Matthew G, et al.
Publicado: (2020)

Causality and causal inference in epidemiology: the need for a pluralistic approach
por: Vandenbroucke, Jan P, et al.
Publicado: (2016)

SIEVE: joint inference of single-nucleotide variants and cell phylogeny from single-cell DNA sequencing data
por: Kang, Senbai, et al.
Publicado: (2022)

Single-molecule photobleaching reveals increased MET receptor dimerization upon ligand binding in intact cells
por: Dietz, Marina S, et al.
Publicado: (2013)

Inferring gene regulatory network from single-cell transcriptomes with graph autoencoder model
por: Wang, Jiacheng, et al.
Publicado: (2023)

Commentary: The formal approach to quantitative causal inference in epidemiology: misguided or misrepresented?
por: Daniel, Rhian M, et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_c2f3d62lfrf0d6bn2r21h3al5t