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A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling

Plant steroid hormones, brassinosteroids, are essential for growth, development and responses to environmental stresses in plants. Although BR signaling proteins are localized in many organelles, i.e., the plasma membrane, nuclei, endoplasmic reticulum and vacuole, the details regarding the BR signa...

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Autores principales: Bekh-Ochir, Davaapurev, Shimada, Setsuko, Yamagami, Ayumi, Kanda, Satomi, Ogawa, Kenji, Nakazawa, Miki, Matsui, Minami, Sakuta, Masaaki, Osada, Hiroyuki, Asami, Tadao, Nakano, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer-Verlag 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664749/
https://www.ncbi.nlm.nih.gov/pubmed/23494613
http://dx.doi.org/10.1007/s00425-013-1859-3
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author Bekh-Ochir, Davaapurev
Shimada, Setsuko
Yamagami, Ayumi
Kanda, Satomi
Ogawa, Kenji
Nakazawa, Miki
Matsui, Minami
Sakuta, Masaaki
Osada, Hiroyuki
Asami, Tadao
Nakano, Takeshi
author_facet Bekh-Ochir, Davaapurev
Shimada, Setsuko
Yamagami, Ayumi
Kanda, Satomi
Ogawa, Kenji
Nakazawa, Miki
Matsui, Minami
Sakuta, Masaaki
Osada, Hiroyuki
Asami, Tadao
Nakano, Takeshi
author_sort Bekh-Ochir, Davaapurev
collection PubMed
description Plant steroid hormones, brassinosteroids, are essential for growth, development and responses to environmental stresses in plants. Although BR signaling proteins are localized in many organelles, i.e., the plasma membrane, nuclei, endoplasmic reticulum and vacuole, the details regarding the BR signaling pathway from perception at the cellular membrane receptor BRASSINOSTEROID INSENSITIVE 1 (BRI1) to nuclear events include several steps. Brz (Brz220) is a specific inhibitor of BR biosynthesis. In this study, we used Brz-mediated chemical genetics to identify Brz-insensitive-long hypocotyls 2-1D (bil2-1D). The BIL2 gene encodes a mitochondrial-localized DnaJ/Heat shock protein 40 (DnaJ/Hsp40) family, which is involved in protein folding. BIL2-overexpression plants (BIL2-OX) showed cell elongation under Brz treatment, increasing the growth of plant inflorescence and roots, the regulation of BR-responsive gene expression and suppression against the dwarfed BRI1-deficient mutant. BIL2-OX also showed resistance against the mitochondrial ATPase inhibitor oligomycin and higher levels of exogenous ATP compared with wild-type plants. BIL2 participates in resistance against salinity stress and strong light stress. Our results indicate that BIL2 induces cell elongation during BR signaling through the promotion of ATP synthesis in mitochondria. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00425-013-1859-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-36647492013-06-03 A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling Bekh-Ochir, Davaapurev Shimada, Setsuko Yamagami, Ayumi Kanda, Satomi Ogawa, Kenji Nakazawa, Miki Matsui, Minami Sakuta, Masaaki Osada, Hiroyuki Asami, Tadao Nakano, Takeshi Planta Original Article Plant steroid hormones, brassinosteroids, are essential for growth, development and responses to environmental stresses in plants. Although BR signaling proteins are localized in many organelles, i.e., the plasma membrane, nuclei, endoplasmic reticulum and vacuole, the details regarding the BR signaling pathway from perception at the cellular membrane receptor BRASSINOSTEROID INSENSITIVE 1 (BRI1) to nuclear events include several steps. Brz (Brz220) is a specific inhibitor of BR biosynthesis. In this study, we used Brz-mediated chemical genetics to identify Brz-insensitive-long hypocotyls 2-1D (bil2-1D). The BIL2 gene encodes a mitochondrial-localized DnaJ/Heat shock protein 40 (DnaJ/Hsp40) family, which is involved in protein folding. BIL2-overexpression plants (BIL2-OX) showed cell elongation under Brz treatment, increasing the growth of plant inflorescence and roots, the regulation of BR-responsive gene expression and suppression against the dwarfed BRI1-deficient mutant. BIL2-OX also showed resistance against the mitochondrial ATPase inhibitor oligomycin and higher levels of exogenous ATP compared with wild-type plants. BIL2 participates in resistance against salinity stress and strong light stress. Our results indicate that BIL2 induces cell elongation during BR signaling through the promotion of ATP synthesis in mitochondria. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00425-013-1859-3) contains supplementary material, which is available to authorized users. Springer-Verlag 2013-03-15 2013 /pmc/articles/PMC3664749/ /pubmed/23494613 http://dx.doi.org/10.1007/s00425-013-1859-3 Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Original Article
Bekh-Ochir, Davaapurev
Shimada, Setsuko
Yamagami, Ayumi
Kanda, Satomi
Ogawa, Kenji
Nakazawa, Miki
Matsui, Minami
Sakuta, Masaaki
Osada, Hiroyuki
Asami, Tadao
Nakano, Takeshi
A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling
title A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling
title_full A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling
title_fullStr A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling
title_full_unstemmed A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling
title_short A novel mitochondrial DnaJ/Hsp40 family protein BIL2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling
title_sort novel mitochondrial dnaj/hsp40 family protein bil2 promotes plant growth and resistance against environmental stress in brassinosteroid signaling
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664749/
https://www.ncbi.nlm.nih.gov/pubmed/23494613
http://dx.doi.org/10.1007/s00425-013-1859-3
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