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Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish

Bacterial artificial chromosome (BAC) transgenesis and gene/enhancer trapping are effective approaches for identification of genetically defined neuronal populations in the central nervous system (CNS). Here, we applied these techniques to zebrafish (Danio rerio) in order to obtain insights into the...

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Autores principales: Asakawa, Kazuhide, Abe, Gembu, Kawakami, Koichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664770/
https://www.ncbi.nlm.nih.gov/pubmed/23754985
http://dx.doi.org/10.3389/fncir.2013.00100
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author Asakawa, Kazuhide
Abe, Gembu
Kawakami, Koichi
author_facet Asakawa, Kazuhide
Abe, Gembu
Kawakami, Koichi
author_sort Asakawa, Kazuhide
collection PubMed
description Bacterial artificial chromosome (BAC) transgenesis and gene/enhancer trapping are effective approaches for identification of genetically defined neuronal populations in the central nervous system (CNS). Here, we applied these techniques to zebrafish (Danio rerio) in order to obtain insights into the cellular architecture of the axial motor column in vertebrates. First, by using the BAC for the Mnx class homeodomain protein gene mnr2b/mnx2b, we established the mnGFF7 transgenic line expressing the Gal4FF transcriptional activator in a large part of the motor column. Single cell labeling of Gal4FF-expressing cells in the mnGFF7 line enabled a detailed investigation of the morphological characteristics of individual spinal motoneurons, as well as the overall organization of the motor column in a spinal segment. Secondly, from a large-scale gene trap screen, we identified transgenic lines that marked discrete subpopulations of spinal motoneurons with Gal4FF. Molecular characterization of these lines led to the identification of the ADAMTS3 gene, which encodes an evolutionarily conserved ADAMTS family of peptidases and is dynamically expressed in the ventral spinal cord. The transgenic fish established here, along with the identified gene, should facilitate an understanding of the cellular and molecular architecture of the spinal cord motor column and its connection to muscles in vertebrates.
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spelling pubmed-36647702013-06-10 Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish Asakawa, Kazuhide Abe, Gembu Kawakami, Koichi Front Neural Circuits Neuroscience Bacterial artificial chromosome (BAC) transgenesis and gene/enhancer trapping are effective approaches for identification of genetically defined neuronal populations in the central nervous system (CNS). Here, we applied these techniques to zebrafish (Danio rerio) in order to obtain insights into the cellular architecture of the axial motor column in vertebrates. First, by using the BAC for the Mnx class homeodomain protein gene mnr2b/mnx2b, we established the mnGFF7 transgenic line expressing the Gal4FF transcriptional activator in a large part of the motor column. Single cell labeling of Gal4FF-expressing cells in the mnGFF7 line enabled a detailed investigation of the morphological characteristics of individual spinal motoneurons, as well as the overall organization of the motor column in a spinal segment. Secondly, from a large-scale gene trap screen, we identified transgenic lines that marked discrete subpopulations of spinal motoneurons with Gal4FF. Molecular characterization of these lines led to the identification of the ADAMTS3 gene, which encodes an evolutionarily conserved ADAMTS family of peptidases and is dynamically expressed in the ventral spinal cord. The transgenic fish established here, along with the identified gene, should facilitate an understanding of the cellular and molecular architecture of the spinal cord motor column and its connection to muscles in vertebrates. Frontiers Media S.A. 2013-05-28 /pmc/articles/PMC3664770/ /pubmed/23754985 http://dx.doi.org/10.3389/fncir.2013.00100 Text en Copyright © 2013 Asakawa, Abe and Kawakami. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
spellingShingle Neuroscience
Asakawa, Kazuhide
Abe, Gembu
Kawakami, Koichi
Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish
title Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish
title_full Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish
title_fullStr Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish
title_full_unstemmed Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish
title_short Cellular dissection of the spinal cord motor column by BAC transgenesis and gene trapping in zebrafish
title_sort cellular dissection of the spinal cord motor column by bac transgenesis and gene trapping in zebrafish
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664770/
https://www.ncbi.nlm.nih.gov/pubmed/23754985
http://dx.doi.org/10.3389/fncir.2013.00100
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