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Large-scale organization of ribosomal DNA chromatin is regulated by Tip5
The DNase I accessibility and chromatin organization of genes within the nucleus do correlate to their transcriptional activity. Here, we show that both serum starvation and overexpression of Tip5, a key regulator of ribosomal RNA gene (rDNA) repression, dictate DNase I accessibility, facilitate the...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664807/ https://www.ncbi.nlm.nih.gov/pubmed/23580549 http://dx.doi.org/10.1093/nar/gkt218 |
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author | Zillner, Karina Filarsky, Michael Rachow, Katrin Weinberger, Michael Längst, Gernot Németh, Attila |
author_facet | Zillner, Karina Filarsky, Michael Rachow, Katrin Weinberger, Michael Längst, Gernot Németh, Attila |
author_sort | Zillner, Karina |
collection | PubMed |
description | The DNase I accessibility and chromatin organization of genes within the nucleus do correlate to their transcriptional activity. Here, we show that both serum starvation and overexpression of Tip5, a key regulator of ribosomal RNA gene (rDNA) repression, dictate DNase I accessibility, facilitate the association of rDNA with the nuclear matrix and thus regulate large-scale rDNA chromatin organization. Tip5 contains four AT-hooks and a TAM (Tip5/ARBP/MBD) domain, which were proposed to bind matrix-attachment regions (MARs) of the genome. Remarkably, the TAM domain of Tip5 functions as nucleolar localization and nuclear matrix targeting module, whereas AT-hooks do not mediate association with the nuclear matrix, but they are required for nucleolar targeting. These findings suggest a dual role for Tip5’s AT-hooks and TAM domain, targeting the nucleolus and anchoring to the nuclear matrix, and suggest a function for Tip5 in the regulation of higher-order rDNA chromatin structure. |
format | Online Article Text |
id | pubmed-3664807 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-36648072013-05-28 Large-scale organization of ribosomal DNA chromatin is regulated by Tip5 Zillner, Karina Filarsky, Michael Rachow, Katrin Weinberger, Michael Längst, Gernot Németh, Attila Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics The DNase I accessibility and chromatin organization of genes within the nucleus do correlate to their transcriptional activity. Here, we show that both serum starvation and overexpression of Tip5, a key regulator of ribosomal RNA gene (rDNA) repression, dictate DNase I accessibility, facilitate the association of rDNA with the nuclear matrix and thus regulate large-scale rDNA chromatin organization. Tip5 contains four AT-hooks and a TAM (Tip5/ARBP/MBD) domain, which were proposed to bind matrix-attachment regions (MARs) of the genome. Remarkably, the TAM domain of Tip5 functions as nucleolar localization and nuclear matrix targeting module, whereas AT-hooks do not mediate association with the nuclear matrix, but they are required for nucleolar targeting. These findings suggest a dual role for Tip5’s AT-hooks and TAM domain, targeting the nucleolus and anchoring to the nuclear matrix, and suggest a function for Tip5 in the regulation of higher-order rDNA chromatin structure. Oxford University Press 2013-05 2013-04-10 /pmc/articles/PMC3664807/ /pubmed/23580549 http://dx.doi.org/10.1093/nar/gkt218 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Gene Regulation, Chromatin and Epigenetics Zillner, Karina Filarsky, Michael Rachow, Katrin Weinberger, Michael Längst, Gernot Németh, Attila Large-scale organization of ribosomal DNA chromatin is regulated by Tip5 |
title | Large-scale organization of ribosomal DNA chromatin is regulated by Tip5 |
title_full | Large-scale organization of ribosomal DNA chromatin is regulated by Tip5 |
title_fullStr | Large-scale organization of ribosomal DNA chromatin is regulated by Tip5 |
title_full_unstemmed | Large-scale organization of ribosomal DNA chromatin is regulated by Tip5 |
title_short | Large-scale organization of ribosomal DNA chromatin is regulated by Tip5 |
title_sort | large-scale organization of ribosomal dna chromatin is regulated by tip5 |
topic | Gene Regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664807/ https://www.ncbi.nlm.nih.gov/pubmed/23580549 http://dx.doi.org/10.1093/nar/gkt218 |
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