Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs

We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small...

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Detalles Bibliográficos
Autores principales: Casbon, James A., Slatter, Andrew F., Musgrave-Brown, Esther, Osborne, Robert J., Lichtenstein, Conrad P., Brenner, Sydney
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664814/
https://www.ncbi.nlm.nih.gov/pubmed/23580546
http://dx.doi.org/10.1093/nar/gkt228
Descripción
Sumario:We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small number of primer extension steps to rapidly enable uniform sequence coverage of long contiguous sequence targets in large numbers of samples at low cost on desktop next-generation sequencers.

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