Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs

We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small...

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Detalles Bibliográficos
Autores principales: Casbon, James A., Slatter, Andrew F., Musgrave-Brown, Esther, Osborne, Robert J., Lichtenstein, Conrad P., Brenner, Sydney
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2013
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664814/
https://www.ncbi.nlm.nih.gov/pubmed/23580546
http://dx.doi.org/10.1093/nar/gkt228
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author Casbon, James A.
Slatter, Andrew F.
Musgrave-Brown, Esther
Osborne, Robert J.
Lichtenstein, Conrad P.
Brenner, Sydney
author_facet Casbon, James A.
Slatter, Andrew F.
Musgrave-Brown, Esther
Osborne, Robert J.
Lichtenstein, Conrad P.
Brenner, Sydney
author_sort Casbon, James A.
collection PubMed
description We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small number of primer extension steps to rapidly enable uniform sequence coverage of long contiguous sequence targets in large numbers of samples at low cost on desktop next-generation sequencers.
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spelling pubmed-36648142013-05-28 Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs Casbon, James A. Slatter, Andrew F. Musgrave-Brown, Esther Osborne, Robert J. Lichtenstein, Conrad P. Brenner, Sydney Nucleic Acids Res Methods Online We present an intramolecular reaction, Reflex™, to derive shorter, sequencer-ready, daughter polymerase chain reaction products from a pooled population of barcoded long-range polymerase chain reaction products, whilst still preserving the cognate DNA barcodes. Our Reflex workflow needs only a small number of primer extension steps to rapidly enable uniform sequence coverage of long contiguous sequence targets in large numbers of samples at low cost on desktop next-generation sequencers. Oxford University Press 2013-05 2013-04-10 /pmc/articles/PMC3664814/ /pubmed/23580546 http://dx.doi.org/10.1093/nar/gkt228 Text en © The Author(s) 2013. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Methods Online
Casbon, James A.
Slatter, Andrew F.
Musgrave-Brown, Esther
Osborne, Robert J.
Lichtenstein, Conrad P.
Brenner, Sydney
Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs
title Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs
title_full Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs
title_fullStr Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs
title_full_unstemmed Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs
title_short Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs
title_sort reflex: intramolecular barcoding of long-range pcr products for sequencing multiple pooled dnas
topic Methods Online
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3664814/
https://www.ncbi.nlm.nih.gov/pubmed/23580546
http://dx.doi.org/10.1093/nar/gkt228
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