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Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons
We developed a method to reconstruct cell geometry from confocal fluorescence microscopy images of the cytoskeleton. In the method, region growing was implemented twice. First, it was applied to the extracellular regions to differentiate them from intracellular noncytoskeletal regions, which both ap...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665187/ https://www.ncbi.nlm.nih.gov/pubmed/23762186 http://dx.doi.org/10.1155/2013/381356 |
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author | Ujihara, Yoshihiro Nakamura, Masanori Miyazaki, Hiroshi Wada, Shigeo |
author_facet | Ujihara, Yoshihiro Nakamura, Masanori Miyazaki, Hiroshi Wada, Shigeo |
author_sort | Ujihara, Yoshihiro |
collection | PubMed |
description | We developed a method to reconstruct cell geometry from confocal fluorescence microscopy images of the cytoskeleton. In the method, region growing was implemented twice. First, it was applied to the extracellular regions to differentiate them from intracellular noncytoskeletal regions, which both appear black in fluorescence microscopy imagery, and then to cell regions for cell identification. Analysis of morphological parameters revealed significant changes in cell shape associated with cytoskeleton disruption, which offered insight into the mechanical role of the cytoskeleton in maintaining cell shape. The proposed segmentation method is promising for investigations on cell morphological changes with respect to internal cytoskeletal structures. |
format | Online Article Text |
id | pubmed-3665187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-36651872013-06-12 Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons Ujihara, Yoshihiro Nakamura, Masanori Miyazaki, Hiroshi Wada, Shigeo Comput Math Methods Med Research Article We developed a method to reconstruct cell geometry from confocal fluorescence microscopy images of the cytoskeleton. In the method, region growing was implemented twice. First, it was applied to the extracellular regions to differentiate them from intracellular noncytoskeletal regions, which both appear black in fluorescence microscopy imagery, and then to cell regions for cell identification. Analysis of morphological parameters revealed significant changes in cell shape associated with cytoskeleton disruption, which offered insight into the mechanical role of the cytoskeleton in maintaining cell shape. The proposed segmentation method is promising for investigations on cell morphological changes with respect to internal cytoskeletal structures. Hindawi Publishing Corporation 2013 2013-05-12 /pmc/articles/PMC3665187/ /pubmed/23762186 http://dx.doi.org/10.1155/2013/381356 Text en Copyright © 2013 Yoshihiro Ujihara et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Ujihara, Yoshihiro Nakamura, Masanori Miyazaki, Hiroshi Wada, Shigeo Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons |
title | Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons |
title_full | Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons |
title_fullStr | Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons |
title_full_unstemmed | Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons |
title_short | Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons |
title_sort | segmentation and morphometric analysis of cells from fluorescence microscopy images of cytoskeletons |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665187/ https://www.ncbi.nlm.nih.gov/pubmed/23762186 http://dx.doi.org/10.1155/2013/381356 |
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