Cargando…

Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons

We developed a method to reconstruct cell geometry from confocal fluorescence microscopy images of the cytoskeleton. In the method, region growing was implemented twice. First, it was applied to the extracellular regions to differentiate them from intracellular noncytoskeletal regions, which both ap...

Descripción completa

Detalles Bibliográficos
Autores principales: Ujihara, Yoshihiro, Nakamura, Masanori, Miyazaki, Hiroshi, Wada, Shigeo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665187/
https://www.ncbi.nlm.nih.gov/pubmed/23762186
http://dx.doi.org/10.1155/2013/381356
_version_ 1782271224864309248
author Ujihara, Yoshihiro
Nakamura, Masanori
Miyazaki, Hiroshi
Wada, Shigeo
author_facet Ujihara, Yoshihiro
Nakamura, Masanori
Miyazaki, Hiroshi
Wada, Shigeo
author_sort Ujihara, Yoshihiro
collection PubMed
description We developed a method to reconstruct cell geometry from confocal fluorescence microscopy images of the cytoskeleton. In the method, region growing was implemented twice. First, it was applied to the extracellular regions to differentiate them from intracellular noncytoskeletal regions, which both appear black in fluorescence microscopy imagery, and then to cell regions for cell identification. Analysis of morphological parameters revealed significant changes in cell shape associated with cytoskeleton disruption, which offered insight into the mechanical role of the cytoskeleton in maintaining cell shape. The proposed segmentation method is promising for investigations on cell morphological changes with respect to internal cytoskeletal structures.
format Online
Article
Text
id pubmed-3665187
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-36651872013-06-12 Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons Ujihara, Yoshihiro Nakamura, Masanori Miyazaki, Hiroshi Wada, Shigeo Comput Math Methods Med Research Article We developed a method to reconstruct cell geometry from confocal fluorescence microscopy images of the cytoskeleton. In the method, region growing was implemented twice. First, it was applied to the extracellular regions to differentiate them from intracellular noncytoskeletal regions, which both appear black in fluorescence microscopy imagery, and then to cell regions for cell identification. Analysis of morphological parameters revealed significant changes in cell shape associated with cytoskeleton disruption, which offered insight into the mechanical role of the cytoskeleton in maintaining cell shape. The proposed segmentation method is promising for investigations on cell morphological changes with respect to internal cytoskeletal structures. Hindawi Publishing Corporation 2013 2013-05-12 /pmc/articles/PMC3665187/ /pubmed/23762186 http://dx.doi.org/10.1155/2013/381356 Text en Copyright © 2013 Yoshihiro Ujihara et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ujihara, Yoshihiro
Nakamura, Masanori
Miyazaki, Hiroshi
Wada, Shigeo
Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons
title Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons
title_full Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons
title_fullStr Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons
title_full_unstemmed Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons
title_short Segmentation and Morphometric Analysis of Cells from Fluorescence Microscopy Images of Cytoskeletons
title_sort segmentation and morphometric analysis of cells from fluorescence microscopy images of cytoskeletons
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665187/
https://www.ncbi.nlm.nih.gov/pubmed/23762186
http://dx.doi.org/10.1155/2013/381356
work_keys_str_mv AT ujiharayoshihiro segmentationandmorphometricanalysisofcellsfromfluorescencemicroscopyimagesofcytoskeletons
AT nakamuramasanori segmentationandmorphometricanalysisofcellsfromfluorescencemicroscopyimagesofcytoskeletons
AT miyazakihiroshi segmentationandmorphometricanalysisofcellsfromfluorescencemicroscopyimagesofcytoskeletons
AT wadashigeo segmentationandmorphometricanalysisofcellsfromfluorescencemicroscopyimagesofcytoskeletons