Cargando…

The Studies of Chlorogenic Acid Antitumor Mechanism by Gene Chip Detection: The Immune Pathway Gene Expression

Persistently increasing incident of cancer in human beings has served to emphasize the importance of studies on mechanism of antitumor substances. Chlorogenic acid (CA), extracted from folium cortex eucommiae, has been confirmed to have lots of biological activities encompassing inhibition of tumor....

Descripción completa

Detalles Bibliográficos
Autores principales: Kang, Tian Yi, Yang, Hua Rong, Zhang, Jie, Li, Dan, Lin, Jie, Wang, Li, Xu, XiaoPing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665237/
https://www.ncbi.nlm.nih.gov/pubmed/23762780
http://dx.doi.org/10.1155/2013/617243
_version_ 1782271235858628608
author Kang, Tian Yi
Yang, Hua Rong
Zhang, Jie
Li, Dan
Lin, Jie
Wang, Li
Xu, XiaoPing
author_facet Kang, Tian Yi
Yang, Hua Rong
Zhang, Jie
Li, Dan
Lin, Jie
Wang, Li
Xu, XiaoPing
author_sort Kang, Tian Yi
collection PubMed
description Persistently increasing incident of cancer in human beings has served to emphasize the importance of studies on mechanism of antitumor substances. Chlorogenic acid (CA), extracted from folium cortex eucommiae, has been confirmed to have lots of biological activities encompassing inhibition of tumor. However, the anticancer mechanism of CA remains unclear. Here, we have utilized a whole mouse genome oligo microarray (4∗44K) to analyze gene expression level of female BALB/c mice (implanted with EMT-6 sarcoma cells) after treatment with low, medium, and high-dose CA (5 mg/kg, 10 mg/kg, and 20 mg/kg), docetaxel, interferon, and normal saline separately at 6 time points (3rd, 6th, 9th, 12th, 15th, and 18th days after administration). Differentially expressed genes screened out by time-series analysis, GO analysis, and pathway analysis, and four immune-related genes were selected for further confirmation using RT-qPCR. The results demonstrated that CA is able to change gene expression and that the responsive genes (CaN, NFATC2, NFATC2ip, and NFATC3) involved in immune pathways had been significantly upregulated by CA. Expression of immune factors such as IL-2R and IFN-γ can be improved by CA to promote activation and proliferation of T cells, macrophages, and NK cells, thus enhancing their surveillance and killing abilities, further suppressing the growth rate of tumor cells.
format Online
Article
Text
id pubmed-3665237
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-36652372013-06-12 The Studies of Chlorogenic Acid Antitumor Mechanism by Gene Chip Detection: The Immune Pathway Gene Expression Kang, Tian Yi Yang, Hua Rong Zhang, Jie Li, Dan Lin, Jie Wang, Li Xu, XiaoPing J Anal Methods Chem Research Article Persistently increasing incident of cancer in human beings has served to emphasize the importance of studies on mechanism of antitumor substances. Chlorogenic acid (CA), extracted from folium cortex eucommiae, has been confirmed to have lots of biological activities encompassing inhibition of tumor. However, the anticancer mechanism of CA remains unclear. Here, we have utilized a whole mouse genome oligo microarray (4∗44K) to analyze gene expression level of female BALB/c mice (implanted with EMT-6 sarcoma cells) after treatment with low, medium, and high-dose CA (5 mg/kg, 10 mg/kg, and 20 mg/kg), docetaxel, interferon, and normal saline separately at 6 time points (3rd, 6th, 9th, 12th, 15th, and 18th days after administration). Differentially expressed genes screened out by time-series analysis, GO analysis, and pathway analysis, and four immune-related genes were selected for further confirmation using RT-qPCR. The results demonstrated that CA is able to change gene expression and that the responsive genes (CaN, NFATC2, NFATC2ip, and NFATC3) involved in immune pathways had been significantly upregulated by CA. Expression of immune factors such as IL-2R and IFN-γ can be improved by CA to promote activation and proliferation of T cells, macrophages, and NK cells, thus enhancing their surveillance and killing abilities, further suppressing the growth rate of tumor cells. Hindawi Publishing Corporation 2013 2013-05-09 /pmc/articles/PMC3665237/ /pubmed/23762780 http://dx.doi.org/10.1155/2013/617243 Text en Copyright © 2013 Tian Yi Kang et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kang, Tian Yi
Yang, Hua Rong
Zhang, Jie
Li, Dan
Lin, Jie
Wang, Li
Xu, XiaoPing
The Studies of Chlorogenic Acid Antitumor Mechanism by Gene Chip Detection: The Immune Pathway Gene Expression
title The Studies of Chlorogenic Acid Antitumor Mechanism by Gene Chip Detection: The Immune Pathway Gene Expression
title_full The Studies of Chlorogenic Acid Antitumor Mechanism by Gene Chip Detection: The Immune Pathway Gene Expression
title_fullStr The Studies of Chlorogenic Acid Antitumor Mechanism by Gene Chip Detection: The Immune Pathway Gene Expression
title_full_unstemmed The Studies of Chlorogenic Acid Antitumor Mechanism by Gene Chip Detection: The Immune Pathway Gene Expression
title_short The Studies of Chlorogenic Acid Antitumor Mechanism by Gene Chip Detection: The Immune Pathway Gene Expression
title_sort studies of chlorogenic acid antitumor mechanism by gene chip detection: the immune pathway gene expression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665237/
https://www.ncbi.nlm.nih.gov/pubmed/23762780
http://dx.doi.org/10.1155/2013/617243
work_keys_str_mv AT kangtianyi thestudiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT yanghuarong thestudiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT zhangjie thestudiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT lidan thestudiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT linjie thestudiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT wangli thestudiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT xuxiaoping thestudiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT kangtianyi studiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT yanghuarong studiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT zhangjie studiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT lidan studiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT linjie studiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT wangli studiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression
AT xuxiaoping studiesofchlorogenicacidantitumormechanismbygenechipdetectiontheimmunepathwaygeneexpression