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ABC Transport Is Inactivated by the PTS(Ntr) under Potassium Limitation in Rhizobium leguminosarum 3841
PTS(Ntr) is a regulatory phosphotransferase system in many bacteria. Mutation of the PTS(Ntr) enzymes causes pleiotropic growth phenotypes, dry colony morphology and a posttranslational inactivation of ABC transporters in Rhizobium leguminosarum 3841. The PTS(Ntr) proteins EI(Ntr) and 2 copies of EI...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665714/ https://www.ncbi.nlm.nih.gov/pubmed/23724079 http://dx.doi.org/10.1371/journal.pone.0064682 |
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author | Untiet, Verena Karunakaran, Ramakrishnan Krämer, Maria Poole, Philip Priefer, Ursula Prell, Jürgen |
author_facet | Untiet, Verena Karunakaran, Ramakrishnan Krämer, Maria Poole, Philip Priefer, Ursula Prell, Jürgen |
author_sort | Untiet, Verena |
collection | PubMed |
description | PTS(Ntr) is a regulatory phosphotransferase system in many bacteria. Mutation of the PTS(Ntr) enzymes causes pleiotropic growth phenotypes, dry colony morphology and a posttranslational inactivation of ABC transporters in Rhizobium leguminosarum 3841. The PTS(Ntr) proteins EI(Ntr) and 2 copies of EIIA(Ntr) have been described previously. Here we identify the intermediate phosphocarrier protein NPr and show its phosphorylation by EI(Ntr) in vitro. Furthermore we demonstrate that phosphorylation of EI(Ntr) and NPr is required for ABC transport activation and that the N-terminal GAF domain of EI(Ntr) is not required for autophosphorylation. Previous studies have shown that non-phosphorylated EIIA(Ntr) is able to modulate the transcriptional activation of the high affinity potassium transporter KdpABC. In R. leguminosarum 3841 kdpABC expression strictly depends on EIIA(Ntr). Here we demonstrate that under strong potassium limitation ABC transport is inactivated, presumably by non-phosphorylated EIIA(Ntr). This is to our knowledge the first report where PTS(Ntr) dictates an essential cellular function. This is achieved by the inverse regulation of two important ATP dependent transporter classes. |
format | Online Article Text |
id | pubmed-3665714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36657142013-05-30 ABC Transport Is Inactivated by the PTS(Ntr) under Potassium Limitation in Rhizobium leguminosarum 3841 Untiet, Verena Karunakaran, Ramakrishnan Krämer, Maria Poole, Philip Priefer, Ursula Prell, Jürgen PLoS One Research Article PTS(Ntr) is a regulatory phosphotransferase system in many bacteria. Mutation of the PTS(Ntr) enzymes causes pleiotropic growth phenotypes, dry colony morphology and a posttranslational inactivation of ABC transporters in Rhizobium leguminosarum 3841. The PTS(Ntr) proteins EI(Ntr) and 2 copies of EIIA(Ntr) have been described previously. Here we identify the intermediate phosphocarrier protein NPr and show its phosphorylation by EI(Ntr) in vitro. Furthermore we demonstrate that phosphorylation of EI(Ntr) and NPr is required for ABC transport activation and that the N-terminal GAF domain of EI(Ntr) is not required for autophosphorylation. Previous studies have shown that non-phosphorylated EIIA(Ntr) is able to modulate the transcriptional activation of the high affinity potassium transporter KdpABC. In R. leguminosarum 3841 kdpABC expression strictly depends on EIIA(Ntr). Here we demonstrate that under strong potassium limitation ABC transport is inactivated, presumably by non-phosphorylated EIIA(Ntr). This is to our knowledge the first report where PTS(Ntr) dictates an essential cellular function. This is achieved by the inverse regulation of two important ATP dependent transporter classes. Public Library of Science 2013-05-28 /pmc/articles/PMC3665714/ /pubmed/23724079 http://dx.doi.org/10.1371/journal.pone.0064682 Text en © 2013 Untiet et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Untiet, Verena Karunakaran, Ramakrishnan Krämer, Maria Poole, Philip Priefer, Ursula Prell, Jürgen ABC Transport Is Inactivated by the PTS(Ntr) under Potassium Limitation in Rhizobium leguminosarum 3841 |
title | ABC Transport Is Inactivated by the PTS(Ntr) under Potassium Limitation in Rhizobium leguminosarum 3841 |
title_full | ABC Transport Is Inactivated by the PTS(Ntr) under Potassium Limitation in Rhizobium leguminosarum 3841 |
title_fullStr | ABC Transport Is Inactivated by the PTS(Ntr) under Potassium Limitation in Rhizobium leguminosarum 3841 |
title_full_unstemmed | ABC Transport Is Inactivated by the PTS(Ntr) under Potassium Limitation in Rhizobium leguminosarum 3841 |
title_short | ABC Transport Is Inactivated by the PTS(Ntr) under Potassium Limitation in Rhizobium leguminosarum 3841 |
title_sort | abc transport is inactivated by the pts(ntr) under potassium limitation in rhizobium leguminosarum 3841 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665714/ https://www.ncbi.nlm.nih.gov/pubmed/23724079 http://dx.doi.org/10.1371/journal.pone.0064682 |
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