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Deciphering the Unique MicroRNA Signature in Human Esophageal Adenocarcinoma

BACKGROUND AND METHODS: Esophageal adenocarcinoma (EAC) is characterized by a steep rise in incidence rates in the Western population. The unique miRNA signature that distinguishes EAC from other upper gastrointestinal cancers remains unclear. Herein, we performed a comprehensive microarray profilin...

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Autores principales: Saad, Rama, Chen, Zheng, Zhu, Shoumin, Jia, Peilin, Zhao, Zhongming, Washington, M. Kay, Belkhiri, Abbes, El-Rifai, Wael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665888/
https://www.ncbi.nlm.nih.gov/pubmed/23724052
http://dx.doi.org/10.1371/journal.pone.0064463
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author Saad, Rama
Chen, Zheng
Zhu, Shoumin
Jia, Peilin
Zhao, Zhongming
Washington, M. Kay
Belkhiri, Abbes
El-Rifai, Wael
author_facet Saad, Rama
Chen, Zheng
Zhu, Shoumin
Jia, Peilin
Zhao, Zhongming
Washington, M. Kay
Belkhiri, Abbes
El-Rifai, Wael
author_sort Saad, Rama
collection PubMed
description BACKGROUND AND METHODS: Esophageal adenocarcinoma (EAC) is characterized by a steep rise in incidence rates in the Western population. The unique miRNA signature that distinguishes EAC from other upper gastrointestinal cancers remains unclear. Herein, we performed a comprehensive microarray profiling for the specific miRNA signature associated with EAC. We validated this signature by qRT-PCR. RESULTS: Microarray analysis showed that 21 miRNAs were consistently deregulated in EAC. miR-194, miR-192, miR-200a, miR-21, miR-203, miR-205, miR-133b, and miR-31 were selected for validation using 46 normal squamous (NS), 23 Barrett’s esophagus (BE), 17 Barrett’s high grade dysplasia (HGD), 34 EAC, 33 gastric adenocarcinoma (GC), and 45 normal gastric (NG) tissues. The qRT-PCR analysis indicated that 2 miRNAs (miR-21 and miR-133b) were deregulated in both EAC and GC, and 6 miRNAs (up-regulated: miR-194, miR-31, miR-192, and miR-200a; down-regulated: miR-203 and miR-205) in EAC, as compared to BE but not in GC, indicating their potential unique role in EAC. Our data showed that miR-194, miR-192, miR-21, and miR-31 were up-regulated in BE adjacent to HGD lesions relative to isolated BE samples. Analysis of clinicopathological features indicated that down-regulation of miR-203 is significantly associated with progression and tumor stages in EAC. Interestingly, the overexpression levels of miR-194, miR-200a, and miR-192 were significantly higher in early EAC stages, suggesting that these miRNAs may be involved in EAC tumor development rather than progression. CONCLUSION: Our findings demonstrate the presence of a unique miRNA signature for EAC. This may provide some clues for the distinct molecular features of EAC to be considered in future studies of the role of miRNAs in EAC and their utility as disease biomarkers.
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spelling pubmed-36658882013-05-30 Deciphering the Unique MicroRNA Signature in Human Esophageal Adenocarcinoma Saad, Rama Chen, Zheng Zhu, Shoumin Jia, Peilin Zhao, Zhongming Washington, M. Kay Belkhiri, Abbes El-Rifai, Wael PLoS One Research Article BACKGROUND AND METHODS: Esophageal adenocarcinoma (EAC) is characterized by a steep rise in incidence rates in the Western population. The unique miRNA signature that distinguishes EAC from other upper gastrointestinal cancers remains unclear. Herein, we performed a comprehensive microarray profiling for the specific miRNA signature associated with EAC. We validated this signature by qRT-PCR. RESULTS: Microarray analysis showed that 21 miRNAs were consistently deregulated in EAC. miR-194, miR-192, miR-200a, miR-21, miR-203, miR-205, miR-133b, and miR-31 were selected for validation using 46 normal squamous (NS), 23 Barrett’s esophagus (BE), 17 Barrett’s high grade dysplasia (HGD), 34 EAC, 33 gastric adenocarcinoma (GC), and 45 normal gastric (NG) tissues. The qRT-PCR analysis indicated that 2 miRNAs (miR-21 and miR-133b) were deregulated in both EAC and GC, and 6 miRNAs (up-regulated: miR-194, miR-31, miR-192, and miR-200a; down-regulated: miR-203 and miR-205) in EAC, as compared to BE but not in GC, indicating their potential unique role in EAC. Our data showed that miR-194, miR-192, miR-21, and miR-31 were up-regulated in BE adjacent to HGD lesions relative to isolated BE samples. Analysis of clinicopathological features indicated that down-regulation of miR-203 is significantly associated with progression and tumor stages in EAC. Interestingly, the overexpression levels of miR-194, miR-200a, and miR-192 were significantly higher in early EAC stages, suggesting that these miRNAs may be involved in EAC tumor development rather than progression. CONCLUSION: Our findings demonstrate the presence of a unique miRNA signature for EAC. This may provide some clues for the distinct molecular features of EAC to be considered in future studies of the role of miRNAs in EAC and their utility as disease biomarkers. Public Library of Science 2013-05-28 /pmc/articles/PMC3665888/ /pubmed/23724052 http://dx.doi.org/10.1371/journal.pone.0064463 Text en © 2013 Saad et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Saad, Rama
Chen, Zheng
Zhu, Shoumin
Jia, Peilin
Zhao, Zhongming
Washington, M. Kay
Belkhiri, Abbes
El-Rifai, Wael
Deciphering the Unique MicroRNA Signature in Human Esophageal Adenocarcinoma
title Deciphering the Unique MicroRNA Signature in Human Esophageal Adenocarcinoma
title_full Deciphering the Unique MicroRNA Signature in Human Esophageal Adenocarcinoma
title_fullStr Deciphering the Unique MicroRNA Signature in Human Esophageal Adenocarcinoma
title_full_unstemmed Deciphering the Unique MicroRNA Signature in Human Esophageal Adenocarcinoma
title_short Deciphering the Unique MicroRNA Signature in Human Esophageal Adenocarcinoma
title_sort deciphering the unique microrna signature in human esophageal adenocarcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3665888/
https://www.ncbi.nlm.nih.gov/pubmed/23724052
http://dx.doi.org/10.1371/journal.pone.0064463
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