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Genome-Wide Scan of the Gene Expression Kinetics of Salmonella enterica Serovar Typhi during Hyperosmotic Stress

Salmonella enterica serovar Typhi is a human enteroinvasive pathogen that can overcome the stress caused by the high osmolarity of the human small intestine and cause systemic infection. To investigate the global transcriptional regulations of S. enterica serovar Typhi exposed to a hyperosmotic envi...

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Autores principales: Huang, Xinxiang, Xu, Huaxi, Sun, Xiaosong, Ohkusu, Kiyofumi, Kawamura, Yoshiaki, Ezaki, Takayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2007
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3666050/
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author Huang, Xinxiang
Xu, Huaxi
Sun, Xiaosong
Ohkusu, Kiyofumi
Kawamura, Yoshiaki
Ezaki, Takayuki
author_facet Huang, Xinxiang
Xu, Huaxi
Sun, Xiaosong
Ohkusu, Kiyofumi
Kawamura, Yoshiaki
Ezaki, Takayuki
author_sort Huang, Xinxiang
collection PubMed
description Salmonella enterica serovar Typhi is a human enteroinvasive pathogen that can overcome the stress caused by the high osmolarity of the human small intestine and cause systemic infection. To investigate the global transcriptional regulations of S. enterica serovar Typhi exposed to a hyperosmotic environment, a genomic oligo-DNA microarray containing 4474 Salmonella genes was prepared. A wild strain of S. enterica serovar Typhi GIFU10007 was grown in LB medium containing 50 mM NaCl to simulate a low osmotic environment. The hyperosmotic stress was simulated by an osmotic up-shift, which increased the concentration of NaCl in the LB from 50 mM to 300 mM. Genome-wide gene expressions of S. enterica serovar Typhi at 15 min, 30 min, 60 min, and 120 min after the osmotic up-shift were investigated by the microarray analysis. Gene expression profiles in somewhat later stage (60 ~120 min) of the stress were quite different from those in the early stage (0 ~ 30 min) of the stress. At 120 min after the osmotic stress, the expression levels of 889 genes were obviously changed. However, expression levels of only 382 genes were significantly changed at 15 min after the osmotic stress. The expression levels of most SPI-1 genes associated with invasion of the pathogen were increased at 120 min after the osmotic up-shift, but were not obviously changed at 15 min or 30 min after the osmotic stress. Expressions of a central regulatory gene, phoP, and sigma factor genes rpoE, rpoD, and rpoS were also changed with different profiles during the osmotic stress. These results indicated that the invasive ability of the pathogen is significantly increased after 2 h of hyperosmotic stress, and regulator PhoP and sigma factors RpoE, RpoD appear to participate in the network regulatory mechanisms that benefit the pathogen to adapt hyperosmotic environmental conditions. The later increased invasive ability of S. enterica serovar Typhi after hyperosmotic stress may be one reason why the pathogen performs invading in the distal ileum of human and not in areas of the upper small intestine.
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spelling pubmed-36660502013-05-30 Genome-Wide Scan of the Gene Expression Kinetics of Salmonella enterica Serovar Typhi during Hyperosmotic Stress Huang, Xinxiang Xu, Huaxi Sun, Xiaosong Ohkusu, Kiyofumi Kawamura, Yoshiaki Ezaki, Takayuki Int J Mol Sci Articles Salmonella enterica serovar Typhi is a human enteroinvasive pathogen that can overcome the stress caused by the high osmolarity of the human small intestine and cause systemic infection. To investigate the global transcriptional regulations of S. enterica serovar Typhi exposed to a hyperosmotic environment, a genomic oligo-DNA microarray containing 4474 Salmonella genes was prepared. A wild strain of S. enterica serovar Typhi GIFU10007 was grown in LB medium containing 50 mM NaCl to simulate a low osmotic environment. The hyperosmotic stress was simulated by an osmotic up-shift, which increased the concentration of NaCl in the LB from 50 mM to 300 mM. Genome-wide gene expressions of S. enterica serovar Typhi at 15 min, 30 min, 60 min, and 120 min after the osmotic up-shift were investigated by the microarray analysis. Gene expression profiles in somewhat later stage (60 ~120 min) of the stress were quite different from those in the early stage (0 ~ 30 min) of the stress. At 120 min after the osmotic stress, the expression levels of 889 genes were obviously changed. However, expression levels of only 382 genes were significantly changed at 15 min after the osmotic stress. The expression levels of most SPI-1 genes associated with invasion of the pathogen were increased at 120 min after the osmotic up-shift, but were not obviously changed at 15 min or 30 min after the osmotic stress. Expressions of a central regulatory gene, phoP, and sigma factor genes rpoE, rpoD, and rpoS were also changed with different profiles during the osmotic stress. These results indicated that the invasive ability of the pathogen is significantly increased after 2 h of hyperosmotic stress, and regulator PhoP and sigma factors RpoE, RpoD appear to participate in the network regulatory mechanisms that benefit the pathogen to adapt hyperosmotic environmental conditions. The later increased invasive ability of S. enterica serovar Typhi after hyperosmotic stress may be one reason why the pathogen performs invading in the distal ileum of human and not in areas of the upper small intestine. Molecular Diversity Preservation International (MDPI) 2007-02-16 /pmc/articles/PMC3666050/ Text en © 2007 by MDPI Reproduction is permitted for noncommercial purposes.
spellingShingle Articles
Huang, Xinxiang
Xu, Huaxi
Sun, Xiaosong
Ohkusu, Kiyofumi
Kawamura, Yoshiaki
Ezaki, Takayuki
Genome-Wide Scan of the Gene Expression Kinetics of Salmonella enterica Serovar Typhi during Hyperosmotic Stress
title Genome-Wide Scan of the Gene Expression Kinetics of Salmonella enterica Serovar Typhi during Hyperosmotic Stress
title_full Genome-Wide Scan of the Gene Expression Kinetics of Salmonella enterica Serovar Typhi during Hyperosmotic Stress
title_fullStr Genome-Wide Scan of the Gene Expression Kinetics of Salmonella enterica Serovar Typhi during Hyperosmotic Stress
title_full_unstemmed Genome-Wide Scan of the Gene Expression Kinetics of Salmonella enterica Serovar Typhi during Hyperosmotic Stress
title_short Genome-Wide Scan of the Gene Expression Kinetics of Salmonella enterica Serovar Typhi during Hyperosmotic Stress
title_sort genome-wide scan of the gene expression kinetics of salmonella enterica serovar typhi during hyperosmotic stress
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3666050/
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