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Expression Levels of Th-2 and Th-17 Characteristic Genes in Healthy Tissue Versus Periodontitis
OBJECTIVE: The T helper 17 (Th17) cells have been suggested to have osteoclast activating effects while T helper 2 (Th2) cells are considered to have an osteoprotective role in periodontitis. This study was to compare the markers of Th17 cells (RORC2 and IL-17 genes) with that of Th2 cells (IL-4 and...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tehran University of Medical Sciences
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3666062/ https://www.ncbi.nlm.nih.gov/pubmed/23724200 |
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author | Behfarnia, Parichehr Birang, Reza Pishva, Seyed Sajad Hakemi, Mazdak Ghanjalikhani Khorasani, Mitra Mohammadi |
author_facet | Behfarnia, Parichehr Birang, Reza Pishva, Seyed Sajad Hakemi, Mazdak Ghanjalikhani Khorasani, Mitra Mohammadi |
author_sort | Behfarnia, Parichehr |
collection | PubMed |
description | OBJECTIVE: The T helper 17 (Th17) cells have been suggested to have osteoclast activating effects while T helper 2 (Th2) cells are considered to have an osteoprotective role in periodontitis. This study was to compare the markers of Th17 cells (RORC2 and IL-17 genes) with that of Th2 cells (IL-4 and GATA-3 genes) between healthy and periodontitis tissues. MATERIALS AND METHODS: The samples were obtained from patients with periodontitis and healthy tissues. The mRNA expression levels of IL-17, RORC2, IL-4 and GATA-3 were measured in both groups using quantitative RT-PCR. The results were compared using SPSS 11.0. Correlation coefficient was analyzed by Spearman’s rho test. Mann-Whitney was used to measure the difference between IL-17 and IL-4 as well as RORC2 and GATA-3. RESULTS: In periodontal lesions, the expression levels of all markers were significantly higher than the healthy tissue (p≤ 0.001). The results showed a significant increase in the number of markers of Th17 (RORC2 and IL-17 genes) compared to markers of Th2 (GATA-3, IL-4) in patients with periodontitis compared to controls (p≤0.002). A positive correlation between IL-17 and RORC2 (p≤0.05) and between IL-4 and GATA-3 (p≤0.001) was found. CONCLUSION: The results show that expression levels of IL-4, GATA-3, IL-17 and RORC2 increase significantly in periodontal lesions compared with the controls. In periodontal lesions, IL-17 levels are significantly greater than IL-4, which plays a protective role against alveolar bone loss. |
format | Online Article Text |
id | pubmed-3666062 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Tehran University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-36660622013-05-30 Expression Levels of Th-2 and Th-17 Characteristic Genes in Healthy Tissue Versus Periodontitis Behfarnia, Parichehr Birang, Reza Pishva, Seyed Sajad Hakemi, Mazdak Ghanjalikhani Khorasani, Mitra Mohammadi J Dent (Tehran) Original Article OBJECTIVE: The T helper 17 (Th17) cells have been suggested to have osteoclast activating effects while T helper 2 (Th2) cells are considered to have an osteoprotective role in periodontitis. This study was to compare the markers of Th17 cells (RORC2 and IL-17 genes) with that of Th2 cells (IL-4 and GATA-3 genes) between healthy and periodontitis tissues. MATERIALS AND METHODS: The samples were obtained from patients with periodontitis and healthy tissues. The mRNA expression levels of IL-17, RORC2, IL-4 and GATA-3 were measured in both groups using quantitative RT-PCR. The results were compared using SPSS 11.0. Correlation coefficient was analyzed by Spearman’s rho test. Mann-Whitney was used to measure the difference between IL-17 and IL-4 as well as RORC2 and GATA-3. RESULTS: In periodontal lesions, the expression levels of all markers were significantly higher than the healthy tissue (p≤ 0.001). The results showed a significant increase in the number of markers of Th17 (RORC2 and IL-17 genes) compared to markers of Th2 (GATA-3, IL-4) in patients with periodontitis compared to controls (p≤0.002). A positive correlation between IL-17 and RORC2 (p≤0.05) and between IL-4 and GATA-3 (p≤0.001) was found. CONCLUSION: The results show that expression levels of IL-4, GATA-3, IL-17 and RORC2 increase significantly in periodontal lesions compared with the controls. In periodontal lesions, IL-17 levels are significantly greater than IL-4, which plays a protective role against alveolar bone loss. Tehran University of Medical Sciences 2013-01-31 2013-01 /pmc/articles/PMC3666062/ /pubmed/23724200 Text en Copyright © Dental Research Center, Tehran University of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial 3.0 License (CC BY-NC 3.0), which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article Behfarnia, Parichehr Birang, Reza Pishva, Seyed Sajad Hakemi, Mazdak Ghanjalikhani Khorasani, Mitra Mohammadi Expression Levels of Th-2 and Th-17 Characteristic Genes in Healthy Tissue Versus Periodontitis |
title | Expression Levels of Th-2 and Th-17 Characteristic Genes in Healthy Tissue Versus Periodontitis |
title_full | Expression Levels of Th-2 and Th-17 Characteristic Genes in Healthy Tissue Versus Periodontitis |
title_fullStr | Expression Levels of Th-2 and Th-17 Characteristic Genes in Healthy Tissue Versus Periodontitis |
title_full_unstemmed | Expression Levels of Th-2 and Th-17 Characteristic Genes in Healthy Tissue Versus Periodontitis |
title_short | Expression Levels of Th-2 and Th-17 Characteristic Genes in Healthy Tissue Versus Periodontitis |
title_sort | expression levels of th-2 and th-17 characteristic genes in healthy tissue versus periodontitis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3666062/ https://www.ncbi.nlm.nih.gov/pubmed/23724200 |
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