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A PCR-Based Method to Genotype Mice Knocked Out for All Four CD3 Subunits, the Standard Recipient Strain for Retrogenic TCR/CD3 Bone Marrow Reconstitution Technology

The novel T-cell receptor (TCR)/CD3-retrogenic-reconstitution system represents a very useful strategy for studying TCR/CD3 signaling. Two retroviral vectors containing genes for all six subunits of the TCR/CD3 complex are used to transduce bone marrow precursors and reconstitute lethally irradiated...

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Detalles Bibliográficos
Autores principales:	Ferrer, Alejandro, Schrum, Adam G., Gil, Diana
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Mary Ann Liebert, Inc. 2013
Materias:	Technical Report
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3666262/ https://www.ncbi.nlm.nih.gov/pubmed/23741635 http://dx.doi.org/10.1089/biores.2013.0002

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