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Cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells
Anticancer activities of cinnamic acid derivatives include induction of apoptosis by irreversible DNA damage leading to cell death. The present work aimed to compare the cytotoxic and genotoxic potential of cinnamic acid in human melanoma cell line (HT-144) and human melanocyte cell line derived fro...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3667113/ https://www.ncbi.nlm.nih.gov/pubmed/23701745 http://dx.doi.org/10.1186/1756-9966-32-31 |
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author | Niero, Evandro Luís de Oliveira Machado-Santelli, Gláucia Maria |
author_facet | Niero, Evandro Luís de Oliveira Machado-Santelli, Gláucia Maria |
author_sort | Niero, Evandro Luís de Oliveira |
collection | PubMed |
description | Anticancer activities of cinnamic acid derivatives include induction of apoptosis by irreversible DNA damage leading to cell death. The present work aimed to compare the cytotoxic and genotoxic potential of cinnamic acid in human melanoma cell line (HT-144) and human melanocyte cell line derived from blue nevus (NGM). Viability assay showed that the IC(50) for HT-144 cells was 2.4 mM, while NGM cells were more resistant to the treatment. The growth inhibition was probably associated with DNA damage leading to DNA synthesis inhibition, as shown by BrdU incorporation assay, induction of nuclear aberrations and then apoptosis. The frequency of cell death caused by cinnamic acid was higher in HT-144 cells. Activated-caspase 3 staining showed apoptosis after 24 hours of treatment with cinnamic acid 3.2 mM in HT-144 cells, but not in NGM. We observed microtubules disorganization after cinnamic acid exposure, but this event and cell death seem to be independent according to M30 and tubulin labeling. The frequency of micronucleated HT-144 cells was higher after treatment with cinnamic acid (0.4 and 3.2 mM) when compared to the controls. Cinnamic acid 3.2 mM also increased the frequency of micronucleated NGM cells indicating genotoxic activity of the compound, but the effects were milder. Binucleation and multinucleation counting showed similar results. We conclude that cinnamic acid has effective antiproliferative activity against melanoma cells. However, the increased frequency of micronucleation in NGM cells warrants the possibility of genotoxicity and needs further investigation. |
format | Online Article Text |
id | pubmed-3667113 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-36671132013-05-30 Cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells Niero, Evandro Luís de Oliveira Machado-Santelli, Gláucia Maria J Exp Clin Cancer Res Research Anticancer activities of cinnamic acid derivatives include induction of apoptosis by irreversible DNA damage leading to cell death. The present work aimed to compare the cytotoxic and genotoxic potential of cinnamic acid in human melanoma cell line (HT-144) and human melanocyte cell line derived from blue nevus (NGM). Viability assay showed that the IC(50) for HT-144 cells was 2.4 mM, while NGM cells were more resistant to the treatment. The growth inhibition was probably associated with DNA damage leading to DNA synthesis inhibition, as shown by BrdU incorporation assay, induction of nuclear aberrations and then apoptosis. The frequency of cell death caused by cinnamic acid was higher in HT-144 cells. Activated-caspase 3 staining showed apoptosis after 24 hours of treatment with cinnamic acid 3.2 mM in HT-144 cells, but not in NGM. We observed microtubules disorganization after cinnamic acid exposure, but this event and cell death seem to be independent according to M30 and tubulin labeling. The frequency of micronucleated HT-144 cells was higher after treatment with cinnamic acid (0.4 and 3.2 mM) when compared to the controls. Cinnamic acid 3.2 mM also increased the frequency of micronucleated NGM cells indicating genotoxic activity of the compound, but the effects were milder. Binucleation and multinucleation counting showed similar results. We conclude that cinnamic acid has effective antiproliferative activity against melanoma cells. However, the increased frequency of micronucleation in NGM cells warrants the possibility of genotoxicity and needs further investigation. BioMed Central 2013-05-23 /pmc/articles/PMC3667113/ /pubmed/23701745 http://dx.doi.org/10.1186/1756-9966-32-31 Text en Copyright © 2013 Niero and Machado-Santelli; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Niero, Evandro Luís de Oliveira Machado-Santelli, Gláucia Maria Cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells |
title | Cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells |
title_full | Cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells |
title_fullStr | Cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells |
title_full_unstemmed | Cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells |
title_short | Cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells |
title_sort | cinnamic acid induces apoptotic cell death and cytoskeleton disruption in human melanoma cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3667113/ https://www.ncbi.nlm.nih.gov/pubmed/23701745 http://dx.doi.org/10.1186/1756-9966-32-31 |
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