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Prenatal Exposure to Urban Air Nanoparticles in Mice Causes Altered Neuronal Differentiation and Depression-Like Responses

Emerging evidence suggests that excessive exposure to traffic-derived air pollution during pregnancy may increase the vulnerability to neurodevelopmental alterations that underlie a broad array of neuropsychiatric disorders. We present a mouse model for prenatal exposure to urban freeway nanoparticu...

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Autores principales: Davis, David A., Bortolato, Marco, Godar, Sean C., Sander, Thomas K., Iwata, Nahoko, Pakbin, Payam, Shih, Jean C., Berhane, Kiros, McConnell, Rob, Sioutas, Constantinos, Finch, Caleb E., Morgan, Todd E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3667185/
https://www.ncbi.nlm.nih.gov/pubmed/23734187
http://dx.doi.org/10.1371/journal.pone.0064128
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author Davis, David A.
Bortolato, Marco
Godar, Sean C.
Sander, Thomas K.
Iwata, Nahoko
Pakbin, Payam
Shih, Jean C.
Berhane, Kiros
McConnell, Rob
Sioutas, Constantinos
Finch, Caleb E.
Morgan, Todd E.
author_facet Davis, David A.
Bortolato, Marco
Godar, Sean C.
Sander, Thomas K.
Iwata, Nahoko
Pakbin, Payam
Shih, Jean C.
Berhane, Kiros
McConnell, Rob
Sioutas, Constantinos
Finch, Caleb E.
Morgan, Todd E.
author_sort Davis, David A.
collection PubMed
description Emerging evidence suggests that excessive exposure to traffic-derived air pollution during pregnancy may increase the vulnerability to neurodevelopmental alterations that underlie a broad array of neuropsychiatric disorders. We present a mouse model for prenatal exposure to urban freeway nanoparticulate matter (nPM). In prior studies, we developed a model for adult rodent exposure to re-aerosolized urban nPM which caused inflammatory brain responses with altered neuronal glutamatergic functions. nPMs are collected continuously for one month from a local freeway and stored as an aqueous suspension, prior to re-aerosolization for exposure of mice under controlled dose and duration. This paradigm was used for a pilot study of prenatal nPM impact on neonatal neurons and adult behaviors. Adult C57BL/6J female mice were exposed to re-aerosolized nPM (350 µg/m(3)) or control filtered ambient air for 10 weeks (3×5 hour exposures per week), encompassing gestation and oocyte maturation prior to mating. Prenatal nPM did not alter litter size, pup weight, or postnatal growth. Neonatal cerebral cortex neurons at 24 hours in vitro showed impaired differentiation, with 50% reduction of stage 3 neurons with long neurites and correspondingly more undifferentiated neurons at Stages 0 and 1. Neuron number after 24 hours of culture was not altered by prenatal nPM exposure. Addition of exogenous nPM (2 µg/ml) to the cultures impaired pyramidal neuron Stage 3 differentiation by 60%. Adult males showed increased depression-like responses in the tail-suspension test, but not anxiety-related behaviors. These pilot data suggest that prenatal exposure to nPM can alter neuronal differentiation with gender-specific behavioral sequelae that may be relevant to human prenatal exposure to urban vehicular aerosols.
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spelling pubmed-36671852013-06-03 Prenatal Exposure to Urban Air Nanoparticles in Mice Causes Altered Neuronal Differentiation and Depression-Like Responses Davis, David A. Bortolato, Marco Godar, Sean C. Sander, Thomas K. Iwata, Nahoko Pakbin, Payam Shih, Jean C. Berhane, Kiros McConnell, Rob Sioutas, Constantinos Finch, Caleb E. Morgan, Todd E. PLoS One Research Article Emerging evidence suggests that excessive exposure to traffic-derived air pollution during pregnancy may increase the vulnerability to neurodevelopmental alterations that underlie a broad array of neuropsychiatric disorders. We present a mouse model for prenatal exposure to urban freeway nanoparticulate matter (nPM). In prior studies, we developed a model for adult rodent exposure to re-aerosolized urban nPM which caused inflammatory brain responses with altered neuronal glutamatergic functions. nPMs are collected continuously for one month from a local freeway and stored as an aqueous suspension, prior to re-aerosolization for exposure of mice under controlled dose and duration. This paradigm was used for a pilot study of prenatal nPM impact on neonatal neurons and adult behaviors. Adult C57BL/6J female mice were exposed to re-aerosolized nPM (350 µg/m(3)) or control filtered ambient air for 10 weeks (3×5 hour exposures per week), encompassing gestation and oocyte maturation prior to mating. Prenatal nPM did not alter litter size, pup weight, or postnatal growth. Neonatal cerebral cortex neurons at 24 hours in vitro showed impaired differentiation, with 50% reduction of stage 3 neurons with long neurites and correspondingly more undifferentiated neurons at Stages 0 and 1. Neuron number after 24 hours of culture was not altered by prenatal nPM exposure. Addition of exogenous nPM (2 µg/ml) to the cultures impaired pyramidal neuron Stage 3 differentiation by 60%. Adult males showed increased depression-like responses in the tail-suspension test, but not anxiety-related behaviors. These pilot data suggest that prenatal exposure to nPM can alter neuronal differentiation with gender-specific behavioral sequelae that may be relevant to human prenatal exposure to urban vehicular aerosols. Public Library of Science 2013-05-29 /pmc/articles/PMC3667185/ /pubmed/23734187 http://dx.doi.org/10.1371/journal.pone.0064128 Text en © 2013 Davis et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Davis, David A.
Bortolato, Marco
Godar, Sean C.
Sander, Thomas K.
Iwata, Nahoko
Pakbin, Payam
Shih, Jean C.
Berhane, Kiros
McConnell, Rob
Sioutas, Constantinos
Finch, Caleb E.
Morgan, Todd E.
Prenatal Exposure to Urban Air Nanoparticles in Mice Causes Altered Neuronal Differentiation and Depression-Like Responses
title Prenatal Exposure to Urban Air Nanoparticles in Mice Causes Altered Neuronal Differentiation and Depression-Like Responses
title_full Prenatal Exposure to Urban Air Nanoparticles in Mice Causes Altered Neuronal Differentiation and Depression-Like Responses
title_fullStr Prenatal Exposure to Urban Air Nanoparticles in Mice Causes Altered Neuronal Differentiation and Depression-Like Responses
title_full_unstemmed Prenatal Exposure to Urban Air Nanoparticles in Mice Causes Altered Neuronal Differentiation and Depression-Like Responses
title_short Prenatal Exposure to Urban Air Nanoparticles in Mice Causes Altered Neuronal Differentiation and Depression-Like Responses
title_sort prenatal exposure to urban air nanoparticles in mice causes altered neuronal differentiation and depression-like responses
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3667185/
https://www.ncbi.nlm.nih.gov/pubmed/23734187
http://dx.doi.org/10.1371/journal.pone.0064128
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