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A Universal Oligonucleotide Microarray with a Minimal Number of Probes for the Detection and Identification of Viroids at the Genus Level
A major challenge in the agricultural industry is the development of techniques that can screen plant samples for viroid infection. Microarrays are promising in this regard, as their high throughput nature can potentially allow for the detection of a range of viroids in a single test. In this paper...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3667192/ https://www.ncbi.nlm.nih.gov/pubmed/23734201 http://dx.doi.org/10.1371/journal.pone.0064474 |
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author | Zhang, Yongjiang Yin, Jun Jiang, Dongmei Xin, Yanyan Ding, Fang Deng, Ziniu Wang, Guoping Ma, Xianfeng Li, Fang Li, Guifen Li, Mingfu Li, Shifang Zhu, Shuifang |
author_facet | Zhang, Yongjiang Yin, Jun Jiang, Dongmei Xin, Yanyan Ding, Fang Deng, Ziniu Wang, Guoping Ma, Xianfeng Li, Fang Li, Guifen Li, Mingfu Li, Shifang Zhu, Shuifang |
author_sort | Zhang, Yongjiang |
collection | PubMed |
description | A major challenge in the agricultural industry is the development of techniques that can screen plant samples for viroid infection. Microarrays are promising in this regard, as their high throughput nature can potentially allow for the detection of a range of viroids in a single test. In this paper we present a microarray that can detect a wide spectrum of all 8 reported viroid genera including 37 known plant viroid species. The array was constructed using an automated probe design protocol which generated a minimal number of probes to detect viroids at the genus level. The designed microarray showed a high specificity and sensitivity when tested with a set of standard virus samples. Finally, the microarray was applied to screen infected field samples, with Hop stunt viroid infection identified as the major disease causing pathogen for an infected citrus sample. |
format | Online Article Text |
id | pubmed-3667192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36671922013-06-03 A Universal Oligonucleotide Microarray with a Minimal Number of Probes for the Detection and Identification of Viroids at the Genus Level Zhang, Yongjiang Yin, Jun Jiang, Dongmei Xin, Yanyan Ding, Fang Deng, Ziniu Wang, Guoping Ma, Xianfeng Li, Fang Li, Guifen Li, Mingfu Li, Shifang Zhu, Shuifang PLoS One Research Article A major challenge in the agricultural industry is the development of techniques that can screen plant samples for viroid infection. Microarrays are promising in this regard, as their high throughput nature can potentially allow for the detection of a range of viroids in a single test. In this paper we present a microarray that can detect a wide spectrum of all 8 reported viroid genera including 37 known plant viroid species. The array was constructed using an automated probe design protocol which generated a minimal number of probes to detect viroids at the genus level. The designed microarray showed a high specificity and sensitivity when tested with a set of standard virus samples. Finally, the microarray was applied to screen infected field samples, with Hop stunt viroid infection identified as the major disease causing pathogen for an infected citrus sample. Public Library of Science 2013-05-29 /pmc/articles/PMC3667192/ /pubmed/23734201 http://dx.doi.org/10.1371/journal.pone.0064474 Text en © 2013 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Zhang, Yongjiang Yin, Jun Jiang, Dongmei Xin, Yanyan Ding, Fang Deng, Ziniu Wang, Guoping Ma, Xianfeng Li, Fang Li, Guifen Li, Mingfu Li, Shifang Zhu, Shuifang A Universal Oligonucleotide Microarray with a Minimal Number of Probes for the Detection and Identification of Viroids at the Genus Level |
title | A Universal Oligonucleotide Microarray with a Minimal Number of Probes for the Detection and Identification of Viroids at the Genus Level |
title_full | A Universal Oligonucleotide Microarray with a Minimal Number of Probes for the Detection and Identification of Viroids at the Genus Level |
title_fullStr | A Universal Oligonucleotide Microarray with a Minimal Number of Probes for the Detection and Identification of Viroids at the Genus Level |
title_full_unstemmed | A Universal Oligonucleotide Microarray with a Minimal Number of Probes for the Detection and Identification of Viroids at the Genus Level |
title_short | A Universal Oligonucleotide Microarray with a Minimal Number of Probes for the Detection and Identification of Viroids at the Genus Level |
title_sort | universal oligonucleotide microarray with a minimal number of probes for the detection and identification of viroids at the genus level |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3667192/ https://www.ncbi.nlm.nih.gov/pubmed/23734201 http://dx.doi.org/10.1371/journal.pone.0064474 |
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