Multiple-Locus Variable-Number Tandem-Repeat Analysis of Mycoplasma pneumoniae Clinical Specimens and Proposal for Amendment of MLVA Nomenclature

Mycoplasma pneumoniae is one of the major respiratory bacterial pathogens that cause pneumonia in humans. Multiple-locus variable-number tandem-repeat analysis (MLVA) is currently the most discriminative method for typing M. pneumoniae strains. To better understand the epidemic of M. pneumoniae-rela...

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Autores principales: Sun, Hongmei, Xue, Guanhua, Yan, Chao, Li, Shaoli, Cao, Ling, Yuan, Yi, Zhao, Hanqing, Feng, Yanling, Wang, Liqiong, Fan, Zhaoyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3667773/
https://www.ncbi.nlm.nih.gov/pubmed/23737989
http://dx.doi.org/10.1371/journal.pone.0064607
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author Sun, Hongmei
Xue, Guanhua
Yan, Chao
Li, Shaoli
Cao, Ling
Yuan, Yi
Zhao, Hanqing
Feng, Yanling
Wang, Liqiong
Fan, Zhaoyang
author_facet Sun, Hongmei
Xue, Guanhua
Yan, Chao
Li, Shaoli
Cao, Ling
Yuan, Yi
Zhao, Hanqing
Feng, Yanling
Wang, Liqiong
Fan, Zhaoyang
author_sort Sun, Hongmei
collection PubMed
description Mycoplasma pneumoniae is one of the major respiratory bacterial pathogens that cause pneumonia in humans. Multiple-locus variable-number tandem-repeat analysis (MLVA) is currently the most discriminative method for typing M. pneumoniae strains. To better understand the epidemic of M. pneumoniae-related pneumonia in pediatric patients in Beijing, China, we performed MLVA analysis on 118 specimens collected during an epidemic from 2010–2012. Eleven distinct MLVA types were identified, including four novel types. There was no obvious association of macrolide resistance with any of the genotypes. Considering the instability of VNTR locus Mpn1, we propose an amended MLVA nomenclature system based on the remaining four VNTR loci.
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spelling pubmed-36677732013-06-04 Multiple-Locus Variable-Number Tandem-Repeat Analysis of Mycoplasma pneumoniae Clinical Specimens and Proposal for Amendment of MLVA Nomenclature Sun, Hongmei Xue, Guanhua Yan, Chao Li, Shaoli Cao, Ling Yuan, Yi Zhao, Hanqing Feng, Yanling Wang, Liqiong Fan, Zhaoyang PLoS One Research Article Mycoplasma pneumoniae is one of the major respiratory bacterial pathogens that cause pneumonia in humans. Multiple-locus variable-number tandem-repeat analysis (MLVA) is currently the most discriminative method for typing M. pneumoniae strains. To better understand the epidemic of M. pneumoniae-related pneumonia in pediatric patients in Beijing, China, we performed MLVA analysis on 118 specimens collected during an epidemic from 2010–2012. Eleven distinct MLVA types were identified, including four novel types. There was no obvious association of macrolide resistance with any of the genotypes. Considering the instability of VNTR locus Mpn1, we propose an amended MLVA nomenclature system based on the remaining four VNTR loci. Public Library of Science 2013-05-30 /pmc/articles/PMC3667773/ /pubmed/23737989 http://dx.doi.org/10.1371/journal.pone.0064607 Text en © 2013 Sun et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sun, Hongmei
Xue, Guanhua
Yan, Chao
Li, Shaoli
Cao, Ling
Yuan, Yi
Zhao, Hanqing
Feng, Yanling
Wang, Liqiong
Fan, Zhaoyang
Multiple-Locus Variable-Number Tandem-Repeat Analysis of Mycoplasma pneumoniae Clinical Specimens and Proposal for Amendment of MLVA Nomenclature
title Multiple-Locus Variable-Number Tandem-Repeat Analysis of Mycoplasma pneumoniae Clinical Specimens and Proposal for Amendment of MLVA Nomenclature
title_full Multiple-Locus Variable-Number Tandem-Repeat Analysis of Mycoplasma pneumoniae Clinical Specimens and Proposal for Amendment of MLVA Nomenclature
title_fullStr Multiple-Locus Variable-Number Tandem-Repeat Analysis of Mycoplasma pneumoniae Clinical Specimens and Proposal for Amendment of MLVA Nomenclature
title_full_unstemmed Multiple-Locus Variable-Number Tandem-Repeat Analysis of Mycoplasma pneumoniae Clinical Specimens and Proposal for Amendment of MLVA Nomenclature
title_short Multiple-Locus Variable-Number Tandem-Repeat Analysis of Mycoplasma pneumoniae Clinical Specimens and Proposal for Amendment of MLVA Nomenclature
title_sort multiple-locus variable-number tandem-repeat analysis of mycoplasma pneumoniae clinical specimens and proposal for amendment of mlva nomenclature
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3667773/
https://www.ncbi.nlm.nih.gov/pubmed/23737989
http://dx.doi.org/10.1371/journal.pone.0064607
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