Class I/II hybrid inhibitory oligodeoxynucleotide exerts Th1 and Th2 double immunosuppression

We designed class I/II hybrid inhibitory oligodeoxynucleotides (iODNs), called iSG, and found that the sequence 5′-TTAGGG-3′, which has a six-base loop head structure, and a 3′-oligo (dG)(3–5) tail sequence are important for potent immunosuppressive activity. Interestingly, splenocytes isolated from...

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Detalles Bibliográficos
Autores principales: Ito, Yusuke, Shigemori, Suguru, Sato, Takashi, Shimazu, Tomoyuki, Hatano, Konomi, Otani, Hajime, Kitazawa, Haruki, Shimosato, Takeshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668506/
https://www.ncbi.nlm.nih.gov/pubmed/23847756
http://dx.doi.org/10.1016/j.fob.2012.11.002
Descripción
Sumario:We designed class I/II hybrid inhibitory oligodeoxynucleotides (iODNs), called iSG, and found that the sequence 5′-TTAGGG-3′, which has a six-base loop head structure, and a 3′-oligo (dG)(3–5) tail sequence are important for potent immunosuppressive activity. Interestingly, splenocytes isolated from ovalbumin (OVA)-immunized mice and treated with iSG3 showed suppression of not only interleukin (IL)-6, IL-12p35, IL-12p40, and interferon (IFN) γ mRNA expression, but also IL-4 and IL-13 mRNA expression. Thus, both Th2 and Th1 immune responses can be strongly suppressed by iODNs in splenocytes from allergen-immunized mice, suggesting usefulness in the treatment of diseases induced by over-active immune activation.

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