Calretinin mediates apoptosis in small cell lung cancer cells expressing tetraspanin CD9()

A majority of small cell lung cancer (SCLC) cells lack a metastasis suppressor, tetraspanin CD9, and CD9 expression promotes their apoptosis. By a proteomics-based approach, we compared an SCLC cell line with its CD9 transfectant and found that a calcium-binding neuronal protein, calretinin, is upre...

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Detalles Bibliográficos
Autores principales: He, Ping, Kuhara, Hanako, Tachibana, Isao, Jin, Yingji, Takeda, Yoshito, Tetsumoto, Satoshi, Minami, Toshiyuki, Kohmo, Satoshi, Hirata, Haruhiko, Takahashi, Ryo, Inoue, Koji, Nagatomo, Izumi, Kida, Hiroshi, Kijima, Takashi, Naka, Tetsuji, Morii, Eiichi, Kawase, Ichiro, Kumanogoh, Atsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668526/
https://www.ncbi.nlm.nih.gov/pubmed/23772398
http://dx.doi.org/10.1016/j.fob.2013.04.005
Descripción
Sumario:A majority of small cell lung cancer (SCLC) cells lack a metastasis suppressor, tetraspanin CD9, and CD9 expression promotes their apoptosis. By a proteomics-based approach, we compared an SCLC cell line with its CD9 transfectant and found that a calcium-binding neuronal protein, calretinin, is upregulated in CD9-positive SCLC cells. Ectopic or anticancer drug-induced CD9 expression upregulated calretinin, whereas CD9 knockdown down-regulated calretinin in SCLC cells. When calretinin was knocked down, CD9-positive SCLC cells revealed increased Akt phosphorylation and decreased apoptosis. These results suggest that CD9 positively regulates the expression of calretinin that mediates proapoptotic effect in SCLC cells.

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