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Calretinin mediates apoptosis in small cell lung cancer cells expressing tetraspanin CD9()

A majority of small cell lung cancer (SCLC) cells lack a metastasis suppressor, tetraspanin CD9, and CD9 expression promotes their apoptosis. By a proteomics-based approach, we compared an SCLC cell line with its CD9 transfectant and found that a calcium-binding neuronal protein, calretinin, is upre...

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Autores principales: He, Ping, Kuhara, Hanako, Tachibana, Isao, Jin, Yingji, Takeda, Yoshito, Tetsumoto, Satoshi, Minami, Toshiyuki, Kohmo, Satoshi, Hirata, Haruhiko, Takahashi, Ryo, Inoue, Koji, Nagatomo, Izumi, Kida, Hiroshi, Kijima, Takashi, Naka, Tetsuji, Morii, Eiichi, Kawase, Ichiro, Kumanogoh, Atsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668526/
https://www.ncbi.nlm.nih.gov/pubmed/23772398
http://dx.doi.org/10.1016/j.fob.2013.04.005
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author He, Ping
Kuhara, Hanako
Tachibana, Isao
Jin, Yingji
Takeda, Yoshito
Tetsumoto, Satoshi
Minami, Toshiyuki
Kohmo, Satoshi
Hirata, Haruhiko
Takahashi, Ryo
Inoue, Koji
Nagatomo, Izumi
Kida, Hiroshi
Kijima, Takashi
Naka, Tetsuji
Morii, Eiichi
Kawase, Ichiro
Kumanogoh, Atsushi
author_facet He, Ping
Kuhara, Hanako
Tachibana, Isao
Jin, Yingji
Takeda, Yoshito
Tetsumoto, Satoshi
Minami, Toshiyuki
Kohmo, Satoshi
Hirata, Haruhiko
Takahashi, Ryo
Inoue, Koji
Nagatomo, Izumi
Kida, Hiroshi
Kijima, Takashi
Naka, Tetsuji
Morii, Eiichi
Kawase, Ichiro
Kumanogoh, Atsushi
author_sort He, Ping
collection PubMed
description A majority of small cell lung cancer (SCLC) cells lack a metastasis suppressor, tetraspanin CD9, and CD9 expression promotes their apoptosis. By a proteomics-based approach, we compared an SCLC cell line with its CD9 transfectant and found that a calcium-binding neuronal protein, calretinin, is upregulated in CD9-positive SCLC cells. Ectopic or anticancer drug-induced CD9 expression upregulated calretinin, whereas CD9 knockdown down-regulated calretinin in SCLC cells. When calretinin was knocked down, CD9-positive SCLC cells revealed increased Akt phosphorylation and decreased apoptosis. These results suggest that CD9 positively regulates the expression of calretinin that mediates proapoptotic effect in SCLC cells.
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spelling pubmed-36685262013-06-14 Calretinin mediates apoptosis in small cell lung cancer cells expressing tetraspanin CD9() He, Ping Kuhara, Hanako Tachibana, Isao Jin, Yingji Takeda, Yoshito Tetsumoto, Satoshi Minami, Toshiyuki Kohmo, Satoshi Hirata, Haruhiko Takahashi, Ryo Inoue, Koji Nagatomo, Izumi Kida, Hiroshi Kijima, Takashi Naka, Tetsuji Morii, Eiichi Kawase, Ichiro Kumanogoh, Atsushi FEBS Open Bio Article A majority of small cell lung cancer (SCLC) cells lack a metastasis suppressor, tetraspanin CD9, and CD9 expression promotes their apoptosis. By a proteomics-based approach, we compared an SCLC cell line with its CD9 transfectant and found that a calcium-binding neuronal protein, calretinin, is upregulated in CD9-positive SCLC cells. Ectopic or anticancer drug-induced CD9 expression upregulated calretinin, whereas CD9 knockdown down-regulated calretinin in SCLC cells. When calretinin was knocked down, CD9-positive SCLC cells revealed increased Akt phosphorylation and decreased apoptosis. These results suggest that CD9 positively regulates the expression of calretinin that mediates proapoptotic effect in SCLC cells. Elsevier 2013-05-10 /pmc/articles/PMC3668526/ /pubmed/23772398 http://dx.doi.org/10.1016/j.fob.2013.04.005 Text en © 2013 The Authors http://creativecommons.org/licenses/BY-license/3.0/ This is an open-access article distributed under the terms of the Creative CommonsAttribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
He, Ping
Kuhara, Hanako
Tachibana, Isao
Jin, Yingji
Takeda, Yoshito
Tetsumoto, Satoshi
Minami, Toshiyuki
Kohmo, Satoshi
Hirata, Haruhiko
Takahashi, Ryo
Inoue, Koji
Nagatomo, Izumi
Kida, Hiroshi
Kijima, Takashi
Naka, Tetsuji
Morii, Eiichi
Kawase, Ichiro
Kumanogoh, Atsushi
Calretinin mediates apoptosis in small cell lung cancer cells expressing tetraspanin CD9()
title Calretinin mediates apoptosis in small cell lung cancer cells expressing tetraspanin CD9()
title_full Calretinin mediates apoptosis in small cell lung cancer cells expressing tetraspanin CD9()
title_fullStr Calretinin mediates apoptosis in small cell lung cancer cells expressing tetraspanin CD9()
title_full_unstemmed Calretinin mediates apoptosis in small cell lung cancer cells expressing tetraspanin CD9()
title_short Calretinin mediates apoptosis in small cell lung cancer cells expressing tetraspanin CD9()
title_sort calretinin mediates apoptosis in small cell lung cancer cells expressing tetraspanin cd9()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668526/
https://www.ncbi.nlm.nih.gov/pubmed/23772398
http://dx.doi.org/10.1016/j.fob.2013.04.005
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