Different effects of ppGpp on Escherichia coli DNA replication in vivo and in vitro()

Inhibition of Escherichia coli DNA replication by guanosine tetraphosphate (ppGpp) is demonstrated in vitro. This finding is compatible with impairment of the DnaG primase activity by this nucleotide. However, in agreement to previous reports, we were not able to detect a rapid inhibition of DNA syn...

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Autores principales: Maciąg-Dorszyńska, Monika, Szalewska-Pałasz, Agnieszka, Węgrzyn, Grzegorz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2013
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668537/
https://www.ncbi.nlm.nih.gov/pubmed/23772389
http://dx.doi.org/10.1016/j.fob.2013.03.001
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author Maciąg-Dorszyńska, Monika
Szalewska-Pałasz, Agnieszka
Węgrzyn, Grzegorz
author_facet Maciąg-Dorszyńska, Monika
Szalewska-Pałasz, Agnieszka
Węgrzyn, Grzegorz
author_sort Maciąg-Dorszyńska, Monika
collection PubMed
description Inhibition of Escherichia coli DNA replication by guanosine tetraphosphate (ppGpp) is demonstrated in vitro. This finding is compatible with impairment of the DnaG primase activity by this nucleotide. However, in agreement to previous reports, we were not able to detect a rapid inhibition of DNA synthesis in E. coli cells under the stringent control conditions, when intracellular ppGpp levels increase dramatically. We suggest that the process of ppGpp-mediated inhibition of DnaG activity may be masked in E. coli cells, which could provide a rationale for explanation of differences between ppGpp effects on DNA replication in E. coli and Bacillus subtilis.
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spelling pubmed-36685372013-06-14 Different effects of ppGpp on Escherichia coli DNA replication in vivo and in vitro() Maciąg-Dorszyńska, Monika Szalewska-Pałasz, Agnieszka Węgrzyn, Grzegorz FEBS Open Bio Article Inhibition of Escherichia coli DNA replication by guanosine tetraphosphate (ppGpp) is demonstrated in vitro. This finding is compatible with impairment of the DnaG primase activity by this nucleotide. However, in agreement to previous reports, we were not able to detect a rapid inhibition of DNA synthesis in E. coli cells under the stringent control conditions, when intracellular ppGpp levels increase dramatically. We suggest that the process of ppGpp-mediated inhibition of DnaG activity may be masked in E. coli cells, which could provide a rationale for explanation of differences between ppGpp effects on DNA replication in E. coli and Bacillus subtilis. Elsevier 2013-03-06 /pmc/articles/PMC3668537/ /pubmed/23772389 http://dx.doi.org/10.1016/j.fob.2013.03.001 Text en © 2013 The Authors http://creativecommons.org/licenses/BY-license/3.0/ This is an open-access article distributed under the terms of the Creative CommonsAttribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
spellingShingle Article
Maciąg-Dorszyńska, Monika
Szalewska-Pałasz, Agnieszka
Węgrzyn, Grzegorz
Different effects of ppGpp on Escherichia coli DNA replication in vivo and in vitro()
title Different effects of ppGpp on Escherichia coli DNA replication in vivo and in vitro()
title_full Different effects of ppGpp on Escherichia coli DNA replication in vivo and in vitro()
title_fullStr Different effects of ppGpp on Escherichia coli DNA replication in vivo and in vitro()
title_full_unstemmed Different effects of ppGpp on Escherichia coli DNA replication in vivo and in vitro()
title_short Different effects of ppGpp on Escherichia coli DNA replication in vivo and in vitro()
title_sort different effects of ppgpp on escherichia coli dna replication in vivo and in vitro()
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3668537/
https://www.ncbi.nlm.nih.gov/pubmed/23772389
http://dx.doi.org/10.1016/j.fob.2013.03.001
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AT wegrzyngrzegorz differenteffectsofppgpponescherichiacolidnareplicationinvivoandinvitro

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