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H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells
BACKGROUND: H-rev107 is a member of the HREV107 type II tumor suppressor gene family which includes H-REV107, RIG1, and HRASLS. H-REV107 has been shown to express at high levels in differentiated tissues of post-meiotic testicular germ cells. Prostaglandin D2 (PGD2) is conjectured to induce SRY-rela...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669107/ https://www.ncbi.nlm.nih.gov/pubmed/23687991 http://dx.doi.org/10.1186/1423-0127-20-30 |
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author | Shyu, Rong-Yaun Wu, Chang-Chieh Wang, Chun-Hua Tsai, Tzung-Chieh Wang, Lu-Kai Chen, Mao-Liang Jiang, Shun-Yuan Tsai, Fu-Ming |
author_facet | Shyu, Rong-Yaun Wu, Chang-Chieh Wang, Chun-Hua Tsai, Tzung-Chieh Wang, Lu-Kai Chen, Mao-Liang Jiang, Shun-Yuan Tsai, Fu-Ming |
author_sort | Shyu, Rong-Yaun |
collection | PubMed |
description | BACKGROUND: H-rev107 is a member of the HREV107 type II tumor suppressor gene family which includes H-REV107, RIG1, and HRASLS. H-REV107 has been shown to express at high levels in differentiated tissues of post-meiotic testicular germ cells. Prostaglandin D2 (PGD2) is conjectured to induce SRY-related high-mobility group box 9 (SOX9) expression and subsequent Sertoli cell differentiation. To date, the function of H-rev107 in differentiated testicular cells has not been well defined. RESULTS: In the study, we found that H-rev107 was co-localized with prostaglandin D2 synthase (PTGDS) and enhanced the activity of PTGDS, resulting in increase of PGD2 production in testis cells. Furthermore, when H-rev107 was expressed in human NT2/D1 testicular cancer cells, cell migration and invasion were inhibited. Also, silencing of PTGDS would reduce H-rev107-mediated increase in PGD2, cAMP, and SOX9. Silencing of PTGDS or SOX9 also alleviated H-rev107-mediated suppression of cell migration and invasion. CONCLUSIONS: These results revealed that H-rev107, through PTGDS, suppressed cell migration and invasion. Our data suggest that the PGD2-cAMP-SOX9 signal pathway might play an important role in H-rev107-mediated cancer cell invasion in testes. |
format | Online Article Text |
id | pubmed-3669107 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-36691072013-06-01 H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells Shyu, Rong-Yaun Wu, Chang-Chieh Wang, Chun-Hua Tsai, Tzung-Chieh Wang, Lu-Kai Chen, Mao-Liang Jiang, Shun-Yuan Tsai, Fu-Ming J Biomed Sci Research BACKGROUND: H-rev107 is a member of the HREV107 type II tumor suppressor gene family which includes H-REV107, RIG1, and HRASLS. H-REV107 has been shown to express at high levels in differentiated tissues of post-meiotic testicular germ cells. Prostaglandin D2 (PGD2) is conjectured to induce SRY-related high-mobility group box 9 (SOX9) expression and subsequent Sertoli cell differentiation. To date, the function of H-rev107 in differentiated testicular cells has not been well defined. RESULTS: In the study, we found that H-rev107 was co-localized with prostaglandin D2 synthase (PTGDS) and enhanced the activity of PTGDS, resulting in increase of PGD2 production in testis cells. Furthermore, when H-rev107 was expressed in human NT2/D1 testicular cancer cells, cell migration and invasion were inhibited. Also, silencing of PTGDS would reduce H-rev107-mediated increase in PGD2, cAMP, and SOX9. Silencing of PTGDS or SOX9 also alleviated H-rev107-mediated suppression of cell migration and invasion. CONCLUSIONS: These results revealed that H-rev107, through PTGDS, suppressed cell migration and invasion. Our data suggest that the PGD2-cAMP-SOX9 signal pathway might play an important role in H-rev107-mediated cancer cell invasion in testes. BioMed Central 2013-05-20 /pmc/articles/PMC3669107/ /pubmed/23687991 http://dx.doi.org/10.1186/1423-0127-20-30 Text en Copyright © 2013 Shyu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Shyu, Rong-Yaun Wu, Chang-Chieh Wang, Chun-Hua Tsai, Tzung-Chieh Wang, Lu-Kai Chen, Mao-Liang Jiang, Shun-Yuan Tsai, Fu-Ming H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells |
title | H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells |
title_full | H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells |
title_fullStr | H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells |
title_full_unstemmed | H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells |
title_short | H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells |
title_sort | h-rev107 regulates prostaglandin d2 synthase-mediated suppression of cellular invasion in testicular cancer cells |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669107/ https://www.ncbi.nlm.nih.gov/pubmed/23687991 http://dx.doi.org/10.1186/1423-0127-20-30 |
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