Cargando…

H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells

BACKGROUND: H-rev107 is a member of the HREV107 type II tumor suppressor gene family which includes H-REV107, RIG1, and HRASLS. H-REV107 has been shown to express at high levels in differentiated tissues of post-meiotic testicular germ cells. Prostaglandin D2 (PGD2) is conjectured to induce SRY-rela...

Descripción completa

Detalles Bibliográficos
Autores principales: Shyu, Rong-Yaun, Wu, Chang-Chieh, Wang, Chun-Hua, Tsai, Tzung-Chieh, Wang, Lu-Kai, Chen, Mao-Liang, Jiang, Shun-Yuan, Tsai, Fu-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669107/
https://www.ncbi.nlm.nih.gov/pubmed/23687991
http://dx.doi.org/10.1186/1423-0127-20-30
_version_ 1782271700230995968
author Shyu, Rong-Yaun
Wu, Chang-Chieh
Wang, Chun-Hua
Tsai, Tzung-Chieh
Wang, Lu-Kai
Chen, Mao-Liang
Jiang, Shun-Yuan
Tsai, Fu-Ming
author_facet Shyu, Rong-Yaun
Wu, Chang-Chieh
Wang, Chun-Hua
Tsai, Tzung-Chieh
Wang, Lu-Kai
Chen, Mao-Liang
Jiang, Shun-Yuan
Tsai, Fu-Ming
author_sort Shyu, Rong-Yaun
collection PubMed
description BACKGROUND: H-rev107 is a member of the HREV107 type II tumor suppressor gene family which includes H-REV107, RIG1, and HRASLS. H-REV107 has been shown to express at high levels in differentiated tissues of post-meiotic testicular germ cells. Prostaglandin D2 (PGD2) is conjectured to induce SRY-related high-mobility group box 9 (SOX9) expression and subsequent Sertoli cell differentiation. To date, the function of H-rev107 in differentiated testicular cells has not been well defined. RESULTS: In the study, we found that H-rev107 was co-localized with prostaglandin D2 synthase (PTGDS) and enhanced the activity of PTGDS, resulting in increase of PGD2 production in testis cells. Furthermore, when H-rev107 was expressed in human NT2/D1 testicular cancer cells, cell migration and invasion were inhibited. Also, silencing of PTGDS would reduce H-rev107-mediated increase in PGD2, cAMP, and SOX9. Silencing of PTGDS or SOX9 also alleviated H-rev107-mediated suppression of cell migration and invasion. CONCLUSIONS: These results revealed that H-rev107, through PTGDS, suppressed cell migration and invasion. Our data suggest that the PGD2-cAMP-SOX9 signal pathway might play an important role in H-rev107-mediated cancer cell invasion in testes.
format Online
Article
Text
id pubmed-3669107
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-36691072013-06-01 H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells Shyu, Rong-Yaun Wu, Chang-Chieh Wang, Chun-Hua Tsai, Tzung-Chieh Wang, Lu-Kai Chen, Mao-Liang Jiang, Shun-Yuan Tsai, Fu-Ming J Biomed Sci Research BACKGROUND: H-rev107 is a member of the HREV107 type II tumor suppressor gene family which includes H-REV107, RIG1, and HRASLS. H-REV107 has been shown to express at high levels in differentiated tissues of post-meiotic testicular germ cells. Prostaglandin D2 (PGD2) is conjectured to induce SRY-related high-mobility group box 9 (SOX9) expression and subsequent Sertoli cell differentiation. To date, the function of H-rev107 in differentiated testicular cells has not been well defined. RESULTS: In the study, we found that H-rev107 was co-localized with prostaglandin D2 synthase (PTGDS) and enhanced the activity of PTGDS, resulting in increase of PGD2 production in testis cells. Furthermore, when H-rev107 was expressed in human NT2/D1 testicular cancer cells, cell migration and invasion were inhibited. Also, silencing of PTGDS would reduce H-rev107-mediated increase in PGD2, cAMP, and SOX9. Silencing of PTGDS or SOX9 also alleviated H-rev107-mediated suppression of cell migration and invasion. CONCLUSIONS: These results revealed that H-rev107, through PTGDS, suppressed cell migration and invasion. Our data suggest that the PGD2-cAMP-SOX9 signal pathway might play an important role in H-rev107-mediated cancer cell invasion in testes. BioMed Central 2013-05-20 /pmc/articles/PMC3669107/ /pubmed/23687991 http://dx.doi.org/10.1186/1423-0127-20-30 Text en Copyright © 2013 Shyu et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Shyu, Rong-Yaun
Wu, Chang-Chieh
Wang, Chun-Hua
Tsai, Tzung-Chieh
Wang, Lu-Kai
Chen, Mao-Liang
Jiang, Shun-Yuan
Tsai, Fu-Ming
H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells
title H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells
title_full H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells
title_fullStr H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells
title_full_unstemmed H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells
title_short H-rev107 regulates prostaglandin D2 synthase-mediated suppression of cellular invasion in testicular cancer cells
title_sort h-rev107 regulates prostaglandin d2 synthase-mediated suppression of cellular invasion in testicular cancer cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669107/
https://www.ncbi.nlm.nih.gov/pubmed/23687991
http://dx.doi.org/10.1186/1423-0127-20-30
work_keys_str_mv AT shyurongyaun hrev107regulatesprostaglandind2synthasemediatedsuppressionofcellularinvasionintesticularcancercells
AT wuchangchieh hrev107regulatesprostaglandind2synthasemediatedsuppressionofcellularinvasionintesticularcancercells
AT wangchunhua hrev107regulatesprostaglandind2synthasemediatedsuppressionofcellularinvasionintesticularcancercells
AT tsaitzungchieh hrev107regulatesprostaglandind2synthasemediatedsuppressionofcellularinvasionintesticularcancercells
AT wanglukai hrev107regulatesprostaglandind2synthasemediatedsuppressionofcellularinvasionintesticularcancercells
AT chenmaoliang hrev107regulatesprostaglandind2synthasemediatedsuppressionofcellularinvasionintesticularcancercells
AT jiangshunyuan hrev107regulatesprostaglandind2synthasemediatedsuppressionofcellularinvasionintesticularcancercells
AT tsaifuming hrev107regulatesprostaglandind2synthasemediatedsuppressionofcellularinvasionintesticularcancercells