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Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study
Photobacterium profundum SS9 is a Gram-negative bacterium, originally collected from the Sulu Sea. Its genome consists of two chromosomes and a 80 kb plasmid. Although it can grow under a wide range of pressures, P. profundum grows optimally at 28 MPa and 15°C. Its ability to grow at atmospheric pre...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669370/ https://www.ncbi.nlm.nih.gov/pubmed/23741291 http://dx.doi.org/10.1371/journal.pone.0060897 |
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author | Le Bihan, Thierry Rayner, Joe Roy, Marcia M. Spagnolo, Laura |
author_facet | Le Bihan, Thierry Rayner, Joe Roy, Marcia M. Spagnolo, Laura |
author_sort | Le Bihan, Thierry |
collection | PubMed |
description | Photobacterium profundum SS9 is a Gram-negative bacterium, originally collected from the Sulu Sea. Its genome consists of two chromosomes and a 80 kb plasmid. Although it can grow under a wide range of pressures, P. profundum grows optimally at 28 MPa and 15°C. Its ability to grow at atmospheric pressure allows for both easy genetic manipulation and culture, making it a model organism to study piezophily. Here, we report a shotgun proteomic analysis of P. profundum grown at atmospheric compared to high pressure using label-free quantitation and mass spectrometry analysis. We have identified differentially expressed proteins involved in high pressure adaptation, which have been previously reported using other methods. Proteins involved in key metabolic pathways were also identified as being differentially expressed. Proteins involved in the glycolysis/gluconeogenesis pathway were up-regulated at high pressure. Conversely, several proteins involved in the oxidative phosphorylation pathway were up-regulated at atmospheric pressure. Some of the proteins that were differentially identified are regulated directly in response to the physical impact of pressure. The expression of some proteins involved in nutrient transport or assimilation, are likely to be directly regulated by pressure. In a natural environment, different hydrostatic pressures represent distinct ecosystems with their own particular nutrient limitations and abundances. However, the only variable considered in this study was atmospheric pressure. |
format | Online Article Text |
id | pubmed-3669370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-36693702013-06-05 Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study Le Bihan, Thierry Rayner, Joe Roy, Marcia M. Spagnolo, Laura PLoS One Research Article Photobacterium profundum SS9 is a Gram-negative bacterium, originally collected from the Sulu Sea. Its genome consists of two chromosomes and a 80 kb plasmid. Although it can grow under a wide range of pressures, P. profundum grows optimally at 28 MPa and 15°C. Its ability to grow at atmospheric pressure allows for both easy genetic manipulation and culture, making it a model organism to study piezophily. Here, we report a shotgun proteomic analysis of P. profundum grown at atmospheric compared to high pressure using label-free quantitation and mass spectrometry analysis. We have identified differentially expressed proteins involved in high pressure adaptation, which have been previously reported using other methods. Proteins involved in key metabolic pathways were also identified as being differentially expressed. Proteins involved in the glycolysis/gluconeogenesis pathway were up-regulated at high pressure. Conversely, several proteins involved in the oxidative phosphorylation pathway were up-regulated at atmospheric pressure. Some of the proteins that were differentially identified are regulated directly in response to the physical impact of pressure. The expression of some proteins involved in nutrient transport or assimilation, are likely to be directly regulated by pressure. In a natural environment, different hydrostatic pressures represent distinct ecosystems with their own particular nutrient limitations and abundances. However, the only variable considered in this study was atmospheric pressure. Public Library of Science 2013-05-31 /pmc/articles/PMC3669370/ /pubmed/23741291 http://dx.doi.org/10.1371/journal.pone.0060897 Text en © 2013 Le Bihan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Le Bihan, Thierry Rayner, Joe Roy, Marcia M. Spagnolo, Laura Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study |
title |
Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study |
title_full |
Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study |
title_fullStr |
Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study |
title_full_unstemmed |
Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study |
title_short |
Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study |
title_sort | photobacterium profundum under pressure: a ms-based label-free quantitative proteomics study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669370/ https://www.ncbi.nlm.nih.gov/pubmed/23741291 http://dx.doi.org/10.1371/journal.pone.0060897 |
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