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Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study

Photobacterium profundum SS9 is a Gram-negative bacterium, originally collected from the Sulu Sea. Its genome consists of two chromosomes and a 80 kb plasmid. Although it can grow under a wide range of pressures, P. profundum grows optimally at 28 MPa and 15°C. Its ability to grow at atmospheric pre...

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Detalles Bibliográficos
Autores principales: Le Bihan, Thierry, Rayner, Joe, Roy, Marcia M., Spagnolo, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669370/
https://www.ncbi.nlm.nih.gov/pubmed/23741291
http://dx.doi.org/10.1371/journal.pone.0060897
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author Le Bihan, Thierry
Rayner, Joe
Roy, Marcia M.
Spagnolo, Laura
author_facet Le Bihan, Thierry
Rayner, Joe
Roy, Marcia M.
Spagnolo, Laura
author_sort Le Bihan, Thierry
collection PubMed
description Photobacterium profundum SS9 is a Gram-negative bacterium, originally collected from the Sulu Sea. Its genome consists of two chromosomes and a 80 kb plasmid. Although it can grow under a wide range of pressures, P. profundum grows optimally at 28 MPa and 15°C. Its ability to grow at atmospheric pressure allows for both easy genetic manipulation and culture, making it a model organism to study piezophily. Here, we report a shotgun proteomic analysis of P. profundum grown at atmospheric compared to high pressure using label-free quantitation and mass spectrometry analysis. We have identified differentially expressed proteins involved in high pressure adaptation, which have been previously reported using other methods. Proteins involved in key metabolic pathways were also identified as being differentially expressed. Proteins involved in the glycolysis/gluconeogenesis pathway were up-regulated at high pressure. Conversely, several proteins involved in the oxidative phosphorylation pathway were up-regulated at atmospheric pressure. Some of the proteins that were differentially identified are regulated directly in response to the physical impact of pressure. The expression of some proteins involved in nutrient transport or assimilation, are likely to be directly regulated by pressure. In a natural environment, different hydrostatic pressures represent distinct ecosystems with their own particular nutrient limitations and abundances. However, the only variable considered in this study was atmospheric pressure.
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spelling pubmed-36693702013-06-05 Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study Le Bihan, Thierry Rayner, Joe Roy, Marcia M. Spagnolo, Laura PLoS One Research Article Photobacterium profundum SS9 is a Gram-negative bacterium, originally collected from the Sulu Sea. Its genome consists of two chromosomes and a 80 kb plasmid. Although it can grow under a wide range of pressures, P. profundum grows optimally at 28 MPa and 15°C. Its ability to grow at atmospheric pressure allows for both easy genetic manipulation and culture, making it a model organism to study piezophily. Here, we report a shotgun proteomic analysis of P. profundum grown at atmospheric compared to high pressure using label-free quantitation and mass spectrometry analysis. We have identified differentially expressed proteins involved in high pressure adaptation, which have been previously reported using other methods. Proteins involved in key metabolic pathways were also identified as being differentially expressed. Proteins involved in the glycolysis/gluconeogenesis pathway were up-regulated at high pressure. Conversely, several proteins involved in the oxidative phosphorylation pathway were up-regulated at atmospheric pressure. Some of the proteins that were differentially identified are regulated directly in response to the physical impact of pressure. The expression of some proteins involved in nutrient transport or assimilation, are likely to be directly regulated by pressure. In a natural environment, different hydrostatic pressures represent distinct ecosystems with their own particular nutrient limitations and abundances. However, the only variable considered in this study was atmospheric pressure. Public Library of Science 2013-05-31 /pmc/articles/PMC3669370/ /pubmed/23741291 http://dx.doi.org/10.1371/journal.pone.0060897 Text en © 2013 Le Bihan et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Le Bihan, Thierry
Rayner, Joe
Roy, Marcia M.
Spagnolo, Laura
Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study
title Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study
title_full Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study
title_fullStr Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study
title_full_unstemmed Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study
title_short Photobacterium profundum under Pressure: A MS-Based Label-Free Quantitative Proteomics Study
title_sort photobacterium profundum under pressure: a ms-based label-free quantitative proteomics study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669370/
https://www.ncbi.nlm.nih.gov/pubmed/23741291
http://dx.doi.org/10.1371/journal.pone.0060897
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