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A Continuum Model of Actin Waves in Dictyostelium discoideum

Actin waves are complex dynamical patterns of the dendritic network of filamentous actin in eukaryotes. We developed a model of actin waves in PTEN-deficient Dictyostelium discoideum by deriving an approximation of the dynamics of discrete actin filaments and combining it with a signaling pathway th...

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Detalles Bibliográficos
Autores principales: Khamviwath, Varunyu, Hu, Jifeng, Othmer, Hans G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669376/
https://www.ncbi.nlm.nih.gov/pubmed/23741312
http://dx.doi.org/10.1371/journal.pone.0064272
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author Khamviwath, Varunyu
Hu, Jifeng
Othmer, Hans G.
author_facet Khamviwath, Varunyu
Hu, Jifeng
Othmer, Hans G.
author_sort Khamviwath, Varunyu
collection PubMed
description Actin waves are complex dynamical patterns of the dendritic network of filamentous actin in eukaryotes. We developed a model of actin waves in PTEN-deficient Dictyostelium discoideum by deriving an approximation of the dynamics of discrete actin filaments and combining it with a signaling pathway that controls filament branching. This signaling pathway, together with the actin network, contains a positive feedback loop that drives the actin waves. Our model predicts the structure, composition, and dynamics of waves that are consistent with existing experimental evidence, as well as the biochemical dependence on various protein partners. Simulation suggests that actin waves are initiated when local actin network activity, caused by an independent process, exceeds a certain threshold. Moreover, diffusion of proteins that form a positive feedback loop with the actin network alone is sufficient for propagation of actin waves at the observed speed of [Image: see text]. Decay of the wave back can be caused by scarcity of network components, and the shape of actin waves is highly dependent on the filament disassembly rate. The model allows retraction of actin waves and captures formation of new wave fronts in broken waves. Our results demonstrate that a delicate balance between a positive feedback, filament disassembly, and local availability of network components is essential for the complex dynamics of actin waves.
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spelling pubmed-36693762013-06-05 A Continuum Model of Actin Waves in Dictyostelium discoideum Khamviwath, Varunyu Hu, Jifeng Othmer, Hans G. PLoS One Research Article Actin waves are complex dynamical patterns of the dendritic network of filamentous actin in eukaryotes. We developed a model of actin waves in PTEN-deficient Dictyostelium discoideum by deriving an approximation of the dynamics of discrete actin filaments and combining it with a signaling pathway that controls filament branching. This signaling pathway, together with the actin network, contains a positive feedback loop that drives the actin waves. Our model predicts the structure, composition, and dynamics of waves that are consistent with existing experimental evidence, as well as the biochemical dependence on various protein partners. Simulation suggests that actin waves are initiated when local actin network activity, caused by an independent process, exceeds a certain threshold. Moreover, diffusion of proteins that form a positive feedback loop with the actin network alone is sufficient for propagation of actin waves at the observed speed of [Image: see text]. Decay of the wave back can be caused by scarcity of network components, and the shape of actin waves is highly dependent on the filament disassembly rate. The model allows retraction of actin waves and captures formation of new wave fronts in broken waves. Our results demonstrate that a delicate balance between a positive feedback, filament disassembly, and local availability of network components is essential for the complex dynamics of actin waves. Public Library of Science 2013-05-31 /pmc/articles/PMC3669376/ /pubmed/23741312 http://dx.doi.org/10.1371/journal.pone.0064272 Text en © 2013 Khamviwath et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Khamviwath, Varunyu
Hu, Jifeng
Othmer, Hans G.
A Continuum Model of Actin Waves in Dictyostelium discoideum
title A Continuum Model of Actin Waves in Dictyostelium discoideum
title_full A Continuum Model of Actin Waves in Dictyostelium discoideum
title_fullStr A Continuum Model of Actin Waves in Dictyostelium discoideum
title_full_unstemmed A Continuum Model of Actin Waves in Dictyostelium discoideum
title_short A Continuum Model of Actin Waves in Dictyostelium discoideum
title_sort continuum model of actin waves in dictyostelium discoideum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3669376/
https://www.ncbi.nlm.nih.gov/pubmed/23741312
http://dx.doi.org/10.1371/journal.pone.0064272
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