Cargando…

Ectopic Overexpression of SsCBF1, a CRT/DRE-Binding Factor from the Nightshade Plant Solanum lycopersicoides, Confers Freezing and Salt Tolerance in Transgenic Arabidopsis

The C-repeat (CRT)/dehydration-responsive element (DRE) binding factor (CBF/DREB1) transcription factors play a key role in cold response. However, the detailed roles of many plant CBFs are far from fully understood. A CBF gene (SsCBF1) was isolated from the cold-hardy plant Solanum lycopersicoides....

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Lili, Li, Zhenjun, Li, Jingfu, Wang, Aoxue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3670921/
https://www.ncbi.nlm.nih.gov/pubmed/23755095
http://dx.doi.org/10.1371/journal.pone.0061810
_version_ 1782271909462802432
author Zhang, Lili
Li, Zhenjun
Li, Jingfu
Wang, Aoxue
author_facet Zhang, Lili
Li, Zhenjun
Li, Jingfu
Wang, Aoxue
author_sort Zhang, Lili
collection PubMed
description The C-repeat (CRT)/dehydration-responsive element (DRE) binding factor (CBF/DREB1) transcription factors play a key role in cold response. However, the detailed roles of many plant CBFs are far from fully understood. A CBF gene (SsCBF1) was isolated from the cold-hardy plant Solanum lycopersicoides. A subcellular localization study using GFP fusion protein indicated that SsCBF1 is localized in the nucleus. We delimited the SsCBF1 transcriptional activation domain to the C-terminal segment comprising amino acid residues 193–228 (SsCBF1(193–228)). The expression of SsCBF1 could be dramatically induced by cold, drought and high salinity. Transactivation assays in tobacco leaves revealed that SsCBF1 could specifically bind to the CRT cis-elements in vivo to activate the expression of downstream reporter genes. The ectopic overexpression of SsCBF1 conferred increased freezing and high-salinity tolerance and late flowering phenotype to transgenic Arabidopsis. RNA-sequencing data exhibited that a set of cold and salt stress responsive genes were up-regulated in transgenic Arabidopsis. Our results suggest that SsCBF1 behaves as a typical CBF to contribute to plant freezing tolerance. Increased resistance to high-salinity and late flowering phenotype derived from SsCBF1 OE lines lend more credence to the hypothesis that plant CBFs participate in diverse physiological and biochemical processes related to adverse conditions.
format Online
Article
Text
id pubmed-3670921
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-36709212013-06-10 Ectopic Overexpression of SsCBF1, a CRT/DRE-Binding Factor from the Nightshade Plant Solanum lycopersicoides, Confers Freezing and Salt Tolerance in Transgenic Arabidopsis Zhang, Lili Li, Zhenjun Li, Jingfu Wang, Aoxue PLoS One Research Article The C-repeat (CRT)/dehydration-responsive element (DRE) binding factor (CBF/DREB1) transcription factors play a key role in cold response. However, the detailed roles of many plant CBFs are far from fully understood. A CBF gene (SsCBF1) was isolated from the cold-hardy plant Solanum lycopersicoides. A subcellular localization study using GFP fusion protein indicated that SsCBF1 is localized in the nucleus. We delimited the SsCBF1 transcriptional activation domain to the C-terminal segment comprising amino acid residues 193–228 (SsCBF1(193–228)). The expression of SsCBF1 could be dramatically induced by cold, drought and high salinity. Transactivation assays in tobacco leaves revealed that SsCBF1 could specifically bind to the CRT cis-elements in vivo to activate the expression of downstream reporter genes. The ectopic overexpression of SsCBF1 conferred increased freezing and high-salinity tolerance and late flowering phenotype to transgenic Arabidopsis. RNA-sequencing data exhibited that a set of cold and salt stress responsive genes were up-regulated in transgenic Arabidopsis. Our results suggest that SsCBF1 behaves as a typical CBF to contribute to plant freezing tolerance. Increased resistance to high-salinity and late flowering phenotype derived from SsCBF1 OE lines lend more credence to the hypothesis that plant CBFs participate in diverse physiological and biochemical processes related to adverse conditions. Public Library of Science 2013-06-03 /pmc/articles/PMC3670921/ /pubmed/23755095 http://dx.doi.org/10.1371/journal.pone.0061810 Text en © 2013 Zhang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Zhang, Lili
Li, Zhenjun
Li, Jingfu
Wang, Aoxue
Ectopic Overexpression of SsCBF1, a CRT/DRE-Binding Factor from the Nightshade Plant Solanum lycopersicoides, Confers Freezing and Salt Tolerance in Transgenic Arabidopsis
title Ectopic Overexpression of SsCBF1, a CRT/DRE-Binding Factor from the Nightshade Plant Solanum lycopersicoides, Confers Freezing and Salt Tolerance in Transgenic Arabidopsis
title_full Ectopic Overexpression of SsCBF1, a CRT/DRE-Binding Factor from the Nightshade Plant Solanum lycopersicoides, Confers Freezing and Salt Tolerance in Transgenic Arabidopsis
title_fullStr Ectopic Overexpression of SsCBF1, a CRT/DRE-Binding Factor from the Nightshade Plant Solanum lycopersicoides, Confers Freezing and Salt Tolerance in Transgenic Arabidopsis
title_full_unstemmed Ectopic Overexpression of SsCBF1, a CRT/DRE-Binding Factor from the Nightshade Plant Solanum lycopersicoides, Confers Freezing and Salt Tolerance in Transgenic Arabidopsis
title_short Ectopic Overexpression of SsCBF1, a CRT/DRE-Binding Factor from the Nightshade Plant Solanum lycopersicoides, Confers Freezing and Salt Tolerance in Transgenic Arabidopsis
title_sort ectopic overexpression of sscbf1, a crt/dre-binding factor from the nightshade plant solanum lycopersicoides, confers freezing and salt tolerance in transgenic arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3670921/
https://www.ncbi.nlm.nih.gov/pubmed/23755095
http://dx.doi.org/10.1371/journal.pone.0061810
work_keys_str_mv AT zhanglili ectopicoverexpressionofsscbf1acrtdrebindingfactorfromthenightshadeplantsolanumlycopersicoidesconfersfreezingandsalttoleranceintransgenicarabidopsis
AT lizhenjun ectopicoverexpressionofsscbf1acrtdrebindingfactorfromthenightshadeplantsolanumlycopersicoidesconfersfreezingandsalttoleranceintransgenicarabidopsis
AT lijingfu ectopicoverexpressionofsscbf1acrtdrebindingfactorfromthenightshadeplantsolanumlycopersicoidesconfersfreezingandsalttoleranceintransgenicarabidopsis
AT wangaoxue ectopicoverexpressionofsscbf1acrtdrebindingfactorfromthenightshadeplantsolanumlycopersicoidesconfersfreezingandsalttoleranceintransgenicarabidopsis